AutoChem: Automated solution-phase parallel synthesis and purification via HPLC

Journal of Combinatorial Chemistry

Volumn 2, Issue 5, 2000, Pages 447-449

  Tommasi, Ruben A ^a   Whaley, Louis W ^a   Marepalli, Hanumantha R ^a  

^a NOVARTIS PHARMA AG   (Switzerland)

Author keywords

[No Author keywords available]

Indexed keywords

ANILINE DERIVATIVE;

ARTICLE; AUTOMATION; CHEMISTRY; COMBINATORIAL CHEMISTRY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; INSTRUMENTATION; METHODOLOGY; SYNTHESIS;

ANILINE COMPOUNDS; AUTOMATION; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; COMBINATORIAL CHEMISTRY TECHNIQUES;

EID: 0034265579     PISSN: 15204766     EISSN: None     Source Type: Journal    
DOI: 10.1021/cc000041r     Document Type: Article

References (19)

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13. The system used is a Gilson HPLC setup consisting of the following devices: An Aspec XL autoinjector, a 305 pump, a 306 pump, a 204 fraction collector and a 119 dual wavelength UV detector. The software used to organize the chemistry routines and then the purification is the Gilson Unpoint 1.7 system (or later). Total cost of the system was $75,000.
14. The routines described herein were written in Pascal, using the 719 developers kit, which is available from Gilson, Inc.
15. The AddCom routine performs the following operations: For n reactions and m μL of solution needed per reaction, n x m μL of solution is aspirated from the common reagent rack in one step and then m μL is dispensed equally into each of n vials in the reaction rack.
16. Mobile phase: A = 0.1% TFA in acetonitrile; B = 0.025% TFA in water. Gradient: 10% A for 3 min; 10% to 90% A during 6.7 min; 90% A for 4.6 min; 90% to 10% A in 0.7 min. For an example of a gradient similar to this which was used recently for high throughput library purification, see: Zeng, L.; Wang, X.; Wang, T.; Kassel, D. B. Comb. Chem. High Throughput Screening 1998, 1, 101-111.
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