Fluorogenic 5' Nuclease PCR (Real Time PCR)

Contemporary Topics in Laboratory Animal Science

Volumn 39, Issue 5, 2000, Pages 41-

  Kendall, Lon V ^a   Besselsen, David G ^c   Riley, Lela K ^b  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b UNIVERSITY OF MISSOURI   (United States)

^c NONE

Author keywords

[No Author keywords available]

Indexed keywords

5' NUCLEOTIDASE; DNA; FLUORESCENT DYE; TAQ POLYMERASE;

ANIMAL; ANIMAL DISEASE; ARTICLE; CHEMISTRY; DNA PROBE; EXPERIMENTAL ANIMAL; ISOLATION AND PURIFICATION; METHODOLOGY; NUCLEIC ACID HYBRIDIZATION; POLYMERASE CHAIN REACTION;

5'-NUCLEOTIDASE; ANIMALS; ANIMALS, LABORATORY; DNA; DNA PROBES; FLUORESCENT DYES; NUCLEIC ACID HYBRIDIZATION; POLYMERASE CHAIN REACTION; TAQ POLYMERASE;

EID: 0034265357     PISSN: 10600558     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Review

References (2)

1. Holland, P.M., R.D. Abramson, R. Watson, and D.H. Gelfand. Detection of specific polymerase chain reaction product by utilizing the 5' to 3' exonuclease activity of Thermus aquaticus DNA polymerase. Proc. Natl. Acad. Sci. USA. 88:7276-7280.
2. Livak, K.J., S.J.A. Flood, J. Marmaro, W. Giusti, and K. Deetz. Oligonucleotides with fluorescent dyes at opposite ends provide a quenched probe system useful for detecting PCR product and nucleic acid hybridization. PCR Methods and Applic. 4:357-362.