Growth of NSO cells in protein-free, chemically defined medium

Biotechnology Progress

Volumn 16, Issue 5, 2000, Pages 682-687

  Gorfien, S ^a   Paul, B ^a   Walowitz, J ^a   Keem, R ^a   Biddle, W ^a   Jayme, D ^a  

^a LIFE TECHNOLOGIES INC   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CHOLESTEROL; PROTEIN;

ARTICLE; CELL CULTURE; CELL DIVISION; CULTURE MEDIUM; MULTIPLE MYELOMA; PATHOLOGY;

CELL DIVISION; CHOLESTEROL; CULTURE MEDIA; MULTIPLE MYELOMA; PROTEINS; TUMOR CELLS, CULTURED;

CRYOPRESERVATION; HYBRIDOMA; LIPID DEPENDENCE; PROTEIN FREE MEDIA; RECOMBINANT MYELOMA CELL; STEROL AUXOTROPHY;

CELL DENSITY; CELL GROWTH; CELL LINE; CRYOPRESERVATION; CULTURE MEDIUM; CYCLODEXTRIN; HYBRIDOMA CELL CULTURE; MYELOMA CELL; STEROL;

ADDITIVES; BIOLOGICAL MATERIALS PRESERVATION; CELLS; GROWTH KINETICS; LIPIDS; PROTEINS;

CELL CULTURE;

ANIMALIA; BOVINAE;

EID: 0034257884     PISSN: 87567938     EISSN: None     Source Type: Journal    
DOI: 10.1021/bp000109a     Document Type: Article

References (13)

1. High level expression of recombinant antibody from myeloma cells using glutamine synthetase as an amplifiable selectable marker
2. Cholesterol requirement of NS-1 mouse myeloma cells for growth in serum-free medium
3. Adaptation of cholesterol-requiring NSO mouse myeloma cells to high density growth in a fully defined protein-free and cholesterol-free culture medium
4. Hybridoma growth and antibody production in a protein-free, chemically defined medium
5. Cloning hybridoma cells in a protein-free, chemically defined hybridoma medium
6. Rapid and simple method for determination of cholesterol in processed food
7. The use of serum-free medium for the production of functionally active humanised monoclonal antibody from NSO mouse myeloma cells engineered using glutamine synthetase as a selectable marker
8. Inactivation of viruses and mycoplasmas in fetal bovine serum using 56 °C heat
9. Inactivation of thirty viruses by gamma irradiation
10. Gamma ray inactivation of some animal viruses
11. Virus inactivation by gamma irradiation of fetal bovine serum