Role of tryptophan residues of lipoxygenase-1 in activity, structure and stability: Chemical modification studies with N-bromosuccinimide

Food Chemistry

Volumn 70, Issue 2, 2000, Pages 199-204

  Srinivasulu, Sonati ^a   Appu Rao, A G ^a  

^a CENTRAL FOOD TECHNOLOGICAL RESEARCH INSTITUTE   (India)

Author keywords

Lipoxygenase 1; N Bromosuccinimide; Structure and stability; Tryptophan residues

Indexed keywords

LIPOXYGENASE; N BROMOSUCCINIMIDE; TRYPTOPHAN;

ARTICLE; CHEMICAL MODIFICATION; CIRCULAR DICHROISM; ENZYME ACTIVITY; ENZYME STABILITY; ENZYME STRUCTURE; PROTEIN FOLDING; SOYBEAN; THERMOSTABILITY;

GLYCINE MAX;

EID: 0034256022     PISSN: 03088146     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0308-8146(00)00080-7     Document Type: Article

References (21)

1. Axelrod B., Cheesbrough T.M., Laakso S. Lipoxygenase from soybeans. Methods in Enzymology. 71:1981;441-451.
2. Boyington J.C., Gaffney B.J., Amzel L.M. The three-dimensional structure of an arachidonic acid 15-lipoxygenase. Science. 260:1993;1482-1486.
3. Cupo J.F., Pace C.N. Conformational stability of mixed disulfide derivatives of β-lactoglobulin B. Biochemistry. 22:1983;2654-2658.
4. Feeney R.E. Chemical modification of proteins. comments and perceptives International Journal of Peptide and Protein Research. 29:1987;145-161.
5. Finazzi-agro A., Avigliano L., Egmond M.R., Veldink G.A., Vliegenthart J.F.G. Fluorescence perturbation in soybean lipoxygenase-1. FEBS Letters. 52:1975;73-76.
6. Gardner H.W. Recent investigations into the lipoxygenase pathway of plants. Biochimca et Biophysica Acta. 1084:1991;211-239.
7. Grechkin A. Recent developments in biochemistry of the plant lipoxygenase pathway. Progress in Lipid Research. 37:1998;317-352.
8. Klein B.P., Cohen B.S., Grossman S., King D., Malovany H., Pinsky A. Effect of modification of soybean lipoxygenase-1 with N-bromosuccinimide on linoleate oxidation, pigment bleaching and carbonyl production. Phytochemistry. 24:1985;1903-1906.
9. Klibanov A.M. Stabilization of enzymes against thermal inactivation. Advances in Applied Microbiology. 29:1983;1-28.
10. Lehrer S.S., Leavis P.C. Solute quenching of protein fluorescence. Methods in Enzymology. 49:1978;222-236.
11. Lundblad, R. L., & Noyes, C. M. (1984). Chemical reagents for protein modification. Boca Raton, FL: CRC Press (Vols. 1 & 11).
12. Minor W., Steczko J., Otwinowski Z., Bolin J.T., Walter R., Axelrod B. Crystal structure of soybean lipoxygenase L-1 at 1.4 Å resolution. Biochemistry. 35:1996;10687-10701.
13. Nozaki Y., Tanford C. The solubility of amino acids and two glycine peptides in aqueous ethanol and dioxane solutions. Journal of Biological Chemistry. 246:1971;2211-2217.
14. Rao A.G.A., Cann J.R. A comparative study of the interaction of chloropromazine, trifluoroperazine and promethazine with mouse brain tubilin. Molecular Pharmacology. 19:1981;295-301.
15. Samuelsson B., Dahlen S.E., Lingren J.A., Rouzer C.A., Serhan C.N. Leukotrienes and lipoxins. structures, biosynthesis, and biological effects Science. 237:1987;1171-1176.
16. Schewe T., Rapoport S.M., Kuhn H. Enzymology of reticulocyte lipoxygenase. comparison with other lipoxygenases Advances in Enzymology and Related Areas in Molecular Biology. 58:1986;191-272.
17. Shibata D., Steczko J., Dixon J.E., Hermodson H., Yazdanparast R., Axelrod B. Primary structure of soybean lipoxygenase-1. Journal of Biological Chemistry. 262:1987;10080-10085.
18. Spande T.F., Witkop B. Determination of tryptophan content of the protein with N-bromosuccinimide. Methods in Enzymology. 11:1967;498-506.
19. Srinivasulu S., Rao A.G.A. Kinetic and structural studies on the interaction of surfactants with lipoxygenase-1 from soybean. Journal of Agricultural and Food Chemistry. 41:1993;366-371.
20. Srinivasulu S., Rao A.G.A. Structure and stability studies on interaction of monohydric alcohols with lipoxygenase-1 from soybeans. Journal of Agricultural and Food Chemistry. 43:1995;562-567.
21. Tsou C.L. Relation between modification of functional groups of proteins and their biological activity. 1. A graphical method for the determination of the number and type of essential groups. Scientia Sinica. 11:1962;1535-1538.