High efficiency separation of microbial aggregates using capillary electrophoresis

FEMS Microbiology Letters

Volumn 189, Issue 1, 2000, Pages 39-44

  Schneiderheinze, J M ^a   Armstrong, D W ^a   Schulte, G ^a   Westenberg, D J ^a  

^a MISSOURI UNIVERSITY OF SCIENCE AND TECHNOLOGY   (United States)

Author keywords

Bacterium; Capillary electrophoresis characterization; Polymer; Separation; Yeast

Indexed keywords

ALCALIGENES; ARTICLE; BACTERIUM ISOLATION; CAPILLARY ELECTROPHORESIS; CELL AGGREGATION; ELECTROPHORETIC MOBILITY; MICROCOCCUS LUTEUS; MICROSCOPY; PRIORITY JOURNAL; SACCHAROMYCES CEREVISIAE; TECHNIQUE; ULTRASOUND;

ALCALIGENES; ELECTROPHORESIS, CAPILLARY; MICROBIOLOGICAL TECHNIQUES; MICROCOCCUS LUTEUS; POLYMERS; SACCHAROMYCES CEREVISIAE;

ALCALIGENES FAECALIS; BACTERIA (MICROORGANISMS); MICROCOCCUS LUTEUS; SACCHAROMYCES CEREVISIAE;

EID: 0034255846     PISSN: 03781097     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1097(00)00249-4     Document Type: Article

References (20)

1. Zhu A., Chen Y. High-voltage capillary zone electrophoresis of red blood cells. J. Chromatogr. 470:1989;251-260.
2. Rosenzweig Z., Yeung E.S. Laser-based particle-counting microimmunoassay for the analysis of single human erythrocytes. Anal. Chem. 66:1994;1771-1776.
3. Hogan B.L., Yeung E.S. Determination of intracellular species at the level of a single erythrocyte via capillary electrophoresis with direct and indirect fluorescence detection. Anal. Chem. 64:1992;2841-2845.
4. Cheung N.-H., Yeung E.S. Distribution of sodium and potassium within individual human erythrocytes by pulsed-laser vaporization in a sheath flow. Anal. Chem. 66:1994;929-936.
5. Sonohara R., Muramatsu N., Hiroyuki O., Kondo T. Difference in surface properties between Escherichia coli and Staphylococcus aureus as revealed by electrophoretic mobility measurements. Biophys. Chem. 55:1995;273-277.
6. Hjertén, S. (1977) In: Cell Separation Methods (Bloemendal, H., Ed.), pp. 117-128. Elsevier, Amsterdam.
7. Ebersole R.C., McCormick R.M. Separation and isolation of viable bacteria by capillary zone electrophoresis. BioTechnology. 11:1993;1278-1282.
8. Hjertén S., et al. Carrier-free zone electrophoresis, displacement electrophoresis and isoelectric focusing in a high-performance electrophoresis apparatus. J. Chromatogr. 403:1987;47-61.
9. Okun V.M., Ronacher B., Blaas D., Kenndler E. Analysis of common cold virus (human rhinovirus serotype 2) by capillary zone electrophoresis: the problem of peak identification. Anal. Chem. 71:1999;2028-2032.
10. Grossman P.D., Soane D.S. Orientation effects on the electrophoretic mobility of rod-shaped molecules in free solution. Anal. Chem. 62:1990;1592-1596.
11. Pfetsch A., Welsch T. Determination of the electrophoretic mobility of bacteria and their separation by capillary zone electrophoresis. Fresenius J. Anal. Chem. 395:1994;198-201.
12. Longton R.W., Cole J.S. III, Quinn P.F. Isoelectric focusing of bacteria: species location within an isoelectric focusing column by surface charge. Arch. Oral Biol. 20:1975;103-106.
13. Jaspers E., Overmann J. Separation of bacterial cells by isoelectric focusing, a new method for analysis of complex microbial communities. Appl. Environ. Microbiol. 63:1997;3176-3181.
14. Stinson S.C. Identifying bacteria: looking for a fast track. Chem. Eng. News. 77:1994;36-38.
15. Belgrader P., et al. PCR detection of bacteria in seven minutes. Science. 284:1990;449-450.
16. Armstrong D.W., Schulte G., Schneiderheinze J.M., Westenberg D.J. Separating microbes in the manner of molecules. 1. Capillary electrokinetic approaches. Anal. Chem. 71:1999;5465-5469.
17. Armstrong, D.W. and Schneiderheinze, J.M. (2000) Rapid identification of the bacterial pathogens responsible for urinary tract infections using direct injection CE. Anal. Chem. 72, submitted.
18. Cota-Robles, E.H. and Stein, S.M. (1989) Bacterial cell breakage or lysis. In: Practical Handbook of Microbiology (O'Leary, W.M., Ed.), pp. 563-574. CRC Press, Boca Raton, FL.
19. Singleton, P. (1997) In: Bacteria in Biology, Biotechnology and Medicine, pp. 6-53. John Wiley and Sons, New York.
20. Fletcher, M. (Ed.) (1996) Bacterial Adhesion, pp. 1-89. Wiley-Liss, New York.