Effects of very high antibiotic concentrations on human intervertebral disc cell proliferation, viability, and metabolism in vitro

Spine

Volumn 25, Issue 15, 2000, Pages 1871-1877

  Hoelscher, Gretchen L ^a   Gruber, Helen E ^a   Coldham, Geoff ^a   Grigsby, Jared H ^a   Hanley Jr, Edward N ^a  

^a CAROLINAS MEDICAL CENTER   (United States)

Author keywords

Antibiotics; Anulus; Cytosensor Microphysiometer; Intervertebral disc; Tissue culture

Indexed keywords

ANTIBIOTIC AGENT; CARBON DIOXIDE; CEFAMANDOLE; CEFAZOLIN; GENTAMICIN; LACTIC ACID; THYMIDINE DERIVATIVE; TRYPAN BLUE; VANCOMYCIN;

ACIDIFICATION; ADOLESCENT; ADULT; ANTIBIOTIC PROPHYLAXIS; ARTICLE; CELL METABOLISM; CELL PROLIFERATION; CELL RESPIRATION; CELL VIABILITY; CHILD; CLINICAL ARTICLE; DISKITIS; FEMALE; GLYCOLYSIS; HUMAN; HUMAN CELL; HUMAN TISSUE; INFANT; MALE; METABOLIC RATE; POSTOPERATIVE COMPLICATION; PRIORITY JOURNAL; SPINE SURGERY; TISSUE CULTURE;

ADULT; CELL DIVISION; CELL SURVIVAL; CELLS, CULTURED; CEPHALOSPORINS; DOSE-RESPONSE RELATIONSHIP, DRUG; ENERGY METABOLISM; FEMALE; GENTAMICINS; HUMANS; HYDROGEN-ION CONCENTRATION; INFANT; INTERVERTEBRAL DISK; MALE; MIDDLE AGED; THYMIDINE; TRYPAN BLUE; VANCOMYCIN;

EID: 0034255189     PISSN: 03622436     EISSN: None     Source Type: Journal    
DOI: 10.1097/00007632-200008010-00003     Document Type: Article

References (32)

1. Effective treatment with interferon-α in chronic recurrent multifocal osteomyelitis
2. Discitis following lumbar surgery
3. Antibiotics in the management of inflammatory lesions of the intervertebral discs in young children
4. Heregulin activation of extracellular acidification in mammary carcinoma cells is associated with expression of HER2 and HER3
5. Gentamicin penetration into normal rabbit nucleus pulposus
6. Antibiotic penetration into rabbit nucleus pulposus
7. Iatrogenic discitis: The role of intravenous antibiotics in prevention and treatment: An experimental study
8. The penetration of antibiotics into the normal intervertebral disc
9. Characterization of human neutrophil and endothelial cell ligand-operated extracellular acidification rate by microphysiometry: Impact on reoxygenation
10. Human intervertebral disc cells from the annulus: Three-dimensional culture in agarose or alginate and responsiveness to TGF-β1
11. Characterization and phenotypic stability of human disc cells in vitro
12. The role of prophylactic antibiotics in spinal instrumentation: A rabbit model
13. The effect of prophylactic antibiotics on iatrogenic intervertebral disc infections: A rabbit model
14. A metabolic view of receptor activation in cultured cells following cryopreservation
15. Aminoglycosides
16. Management of postoperative spine infections
17. The cytosensor microphysiometer: Biological applications of silicon technology
18. The light-addressable potentiometric sensor: Principles and biological applications
19. Detection of cell-affecting agents with a silicon biosensor
20. Nerve growth factor stimulates rapid metabolic responses in PC12 cells
21. Tissue distribution of antibiotics in the intervertebral disc
22. The use of antibiotics for wound prophylaxis in spinal surgery
23. Perioperative prophylactic cephazolin in spinal surgery. A double-blind placebo-controlled trial
24. Microbiology and antimicrobial therapy of spinal infections
25. Penetration of glycopeptide antibiotics in nucleus pulposus
26. Real-time evaluation of somatostatis subtype 2 receptor activity employing the technique of cytosensor microphysiometry
27. A new in vitro model to investigate antibiotic penetration of the intervertebral disc
28. Cellular antibiotic pharmacology
29. Interaction of tobramycin and pH in cultured chick tibiae
30. Calcium hydroxide ameliorates tobramycin toxicity in cultured chick tibiae