Metabolic engineering study on the direct fermentation of 2-keto-L-gulonic acid, a key intermediate of L-ascorbic acid in Pseudomonas putida IFO3738

Journal of Bioscience and Bioengineering

Volumn 90, Issue 2, 2000, Pages 223-225

  Shibata, Takashi ^a   Ichikawa, Chiyo ^a   Matsuura, Mitsutaka ^a   Takata, Yoko ^a   Noguchi, Yuji ^a   Saito, Yoshimasa ^a   Yamashita, Michio ^a  

^a ASTELLAS PHARMA INC   (Japan)

Author keywords

2 keto L gulonic acid; Pseudomonas putida; Sorbitol dehydrogenase; Sorbose dehydrogenase; Sorbosone dehydrogenase

Indexed keywords

CELL CULTURE; FERMENTATION; METABOLISM; ORGANIC ACIDS;

METABOLIC ENGINEERING;

VITAMINS;

ASCORBIC ACID; IDITOL DEHYDROGENASE; RECOMBINANT ENZYME; SORBOSE;

ARTICLE; BACTERIAL METABOLISM; BACTERIUM CULTURE; ESCHERICHIA COLI; FERMENTATION; NONHUMAN; PROMOTER REGION; PSEUDOMONAS PUTIDA;

ESCHERICHIA COLI; PSEUDOMONAS PUTIDA;

EID: 0034253240     PISSN: 13891723     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1389-1723(00)80116-4     Document Type: Article

References (9)

1. Synthese der D- and L-Ascorbinsaure (C-Vitamin)
2. Microbial processes for ascorbic acid biosynthesis: A review
3. Cloning of genes for L-sorbose and L-sorbosone dehydrogenases from Gluconobacter oxydans and microbial production of 2-keto-L-gulonate, a precursor of L-ascorbic acid, in a recombinant G. oxydans strain
4. Direct fermentation of 2-keto-L-gulonic acid in recombinant Gluconobacter oxydans
5. Production of 2-keto-L-gulonate, an intermediate in L-ascorbate synthesis, by a genetically modified Erwinia herbicola
6. Conversion of glucose to 2-keto-L-gulonate, an intermediate in L-ascorbate synthesis, by a recombinant strain of Erwinia citreus
7. Four new derivatives of the broad-host-range cloning vector pBBR1MCS, carrying different antibiotic-resistance cassettes
8. Cloning of a gene for D-sorbitol dehydrogenase from Gluconobacter oxydans G624 and expression of the gene in Pseudomonas putida IFO3738
9. Metabolic pathway for 2-keto-L-gulonic acid formation in Gluconobacter melanogenus IFO 3293