Reproducibility of alternative probe synthesis approaches for gene expression profiling with arrays

Journal of Molecular Diagnostics

Volumn 2, Issue 3, 2000, Pages 124-127

  Vernon, Suzanne D ^a   Unger, Elizabeth R ^a   Rajeevan, Mangalathu ^a   Dimulescu, Irina M ^a   Nisenbaum, Rosane ^a   Campbell, Catherine E ^a  

^a CENTERS FOR DISEASE CONTROL AND PREVENTION   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

COMPLEMENTARY DNA; DIGOXIGENIN; MESSENGER RNA;

ARTICLE; CELL CULTURE; CHEMOLUMINESCENCE; DNA MICROARRAY; DNA PROBE; FEMALE; GENE EXPRESSION PROFILING; GENETICS; HUMAN; METHODOLOGY; NUCLEIC ACID HYBRIDIZATION; REPRODUCIBILITY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; STANDARD; STATISTICS; SYNTHESIS;

CHEMILUMINESCENT MEASUREMENTS; DIGOXIGENIN; DNA PROBES; DNA, COMPLEMENTARY; FEMALE; GENE EXPRESSION PROFILING; HUMANS; NUCLEIC ACID HYBRIDIZATION; OLIGONUCLEOTIDE ARRAY SEQUENCE ANALYSIS; REFERENCE STANDARDS; REPRODUCIBILITY OF RESULTS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, MESSENGER; STATISTICS; TUMOR CELLS, CULTURED;

EID: 0034252527     PISSN: 15251578     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1525-1578(10)60626-5     Document Type: Article

References (8)

1. Endege WO, Steinmann KE, Boardman LA, Thibodeau SN, Schlegel R: Representative cDNA libraries and their utility in gene expression profiling. Biotechniques 1999, 26:542-550
2. Van Gelder RN, von Zastrow ME, Yool A, Dement WC, Barchas JD, Eberwine JH: Amplified RNA synthesized from limited quantities of heterogenous cDNA. Proc Natl Acad Sci USA 1990, 87:1663-1667
3. Mahadevappa M, Warrington JA: A high-density probe array sample preparation method using 10- to 100-fold fewer cells. Nature Biotech 1999, 17:1134-1136
4. Chomczynski P, Sacchi N: Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform. Anal Biochem 1987, 162:156-159
5. Rajeevan MS, Dimulescu IM, Unger ER, Vernon SD: Chemiluminescent analysis of gene expression on high-density filter arrays. J Histochem Cytochem 1999, 47:337-342
6. Lin LI-K: A concordance correlation coefficient to evaluate reproducibility. Biometrics 1989, 45:255-268
7. Zhang J, Byrne CD: Differential priming of RNA templates during cDNA synthesis markedly affects both accuracy and reproducibility of quantitative competitive reverse-transcriptase PCR. Biochem J 1999, 15:337:231-241.
8. Brail LH, Jang A, Billia F, Iscove NN, Klamut HJ, Hill RP: Gene expression in individual cells; analysis using global single cell reverse transcription polymerase chain reaction (GSC RT-PCR). Mutat Res 1999, 406:45-54