Production of novel polyclonal antibodies against the cyanobacterial toxin microcystin-LR and their application for the detection and quantification of microcystins and nodularin

Water Research

Volumn 34, Issue 10, 2000, Pages 2761-2769

  Metcalf, J S ^a   Bell, S G ^a   Codd, G A ^a  

^a UNIVERSITY OF DUNDEE   (United Kingdom)

Author keywords

Antibodies; Blue green algae; Cyanobacteria; Immunoassays; Microcystin; Toxins

Indexed keywords

BACTERIAL TOXIN; CYANOGINOSIN; CYANOGINOSIN LR; KEYHOLE LIMPET HEMOCYANIN; NODULARIN; POLYCLONAL ANTIBODY; TOXIN ANTIBODY;

WATER QUALITY; WATER SUPPLY;

ARTICLE; BLUE GREEN ALGA; CROSS REACTION; CYANOBACTERIUM; ENZYME LINKED IMMUNOSORBENT ASSAY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; NONHUMAN; PRIORITY JOURNAL; RABBIT; TOXIN ANALYSIS; WATER ANALYSIS; WATER CONTAMINATION;

ALGAE; CHLOROPHYTA; CYANOBACTERIA; UNCULTURED CYANOBACTERIUM;

EID: 0034237640     PISSN: 00431354     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0043-1354(99)00429-7     Document Type: Article

References (27)

1. An J., Carmichael W.W. Use of a colorimetric protein phosphatase assay and enzyme linked immunoassay for the study of microcystins and nodularin. Toxicon. 12:1994;1495-1507.
2. Beattie K.A., Kaya K., Sano T., Codd G.A. Three dehydrobutyrine-containing microcystins from Nostoc. Phytochem. 47:1998;1289-1292.
3. Bell S.G., Codd G.A. Cyanobacterial toxins and human health. Rev. Med. Microbiol. 5:1994;256-264.
4. Bell S.G., Codd G.A. Detection, analysis and risk assessment of cyanobacterial toxins. Hester R.E., Harrison R.M. Agricultural Chemicals and the Environment, Issues in Environmental Science and Technology, no. 5. 1996;109-122 Royal Society of Chemistry, Cambridge, UK.
5. Bradford M.M. A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:1976;248-254.
6. Brooks W.P., Codd G.A. Immunoassay of hepatotoxic cultures and water blooms of cyanobacteria using Microcystis aeruginosa peptide toxin polyclonal antibodies. Environ. Technol. Lett. 9:1988;1343-1348.
7. Campbell D.L., Lawton L.A., Beattie K.A., Codd G.A. Comparative assessment of the specificity of the brine shrimp and Microtox assays to hepatotoxic (microcystin-LR containing) cyanobacteria. Environ. Toxicol. Water Qual. 9:1994;71-77.
8. Chu F.S., Huang X., Wei R.D., Carmichael W.W. Production and characterisation of antibodies against microcystins. Appl. Environ. Microbiol. 55:1989;1928-1933.
9. Codd G.A. Cyanobacterial toxins: occurrence, properties and biological significance. Wat. Sci. Technol. 32:1995;149-156.
10. Codd G. A., Beattie K. A. and Raggett S. L. (1996) The evaluation of EnviroGard microcystin plate and tube kits. UK Environment Agency R & D Technical Report P47, Environment Agency, Bristol.
11. Codd G.A., Ward C.J., Beattie K.A., Bell S.G. Widening perceptions of the occurrence and significance of cyanobacterial toxins. Peschek G.A., Löffelhardt W., Schmetterer G. The Phototrophic Prokaryotes. 1999;623-632 Kluwer Academic/Plenum, New York, USA.
12. Harlow E., Lane D. Antibodies: A Laboratory Manual. 1988;Cold Spring Harbour Laboratory, Cold Spring Harbour, New York, USA.
13. Jochimsen E.M., Carmichael W.W., An J.S., Cardo D.M., Cookson S.T., Holmes C.E.M., et al. Liver failure and death after exposure to microcystins at a hemodialysis center in Brazil. New England J. Med. 338:1998;873-878.
14. Kfir R., Johannsen E., Botes D.P. Monoclonal antibodies specific for cyanoginosin-LA: preparation and characterisation. Toxicon. 24:1986;543-552.
15. Lawton L.A., Edwards C., Beattie K.A., Pleasance S., Dear G.J., Codd G.A. Isolation and characterization of microcystins from laboratory cultures and environmental samples of Microcystis aeruginosa and from an associated animal toxicosis. Nat. Toxins. 3:1995;50-57.
16. MacKintosh C., Beattie K.A., Klumpp S., Cohen P., Codd G.A. Cyanobacterial microcystin-LR is a potent and specific inhibitor of protein phosphatase 1 and 2A from both mammals and higher plants. FEBS Lett. 264:1990;187-192.
17. Mittenbühler K., Loleit M., Baier W., Fischer B., Sedelmeier E., Jung G., et al. Drug specific antibodies: T-cell epitope-lipopeptide conjugates are potent adjuvants for small antigens in vivo and in vitro. Int. J. Immunopharmacol. 19:1997;277-287.
18. Moorhead G., MacKintosh R.W., Morrice N., Gallagher T., MacKintosh C. Purification of type 1 protein (serine/threonine) phosphatases by microcystin-Sepharose affinity chromatography. FEBS Lett. 356:1994;46-50.
19. Nagata S., Soutome H., Tsutsumi T., Hasegawa A., Sekijima M., Sugamata M., Harada K.-I., Suganuma M., Ueno Y. Novel monoclonal antibodies against microcystin and their protective activity for hepatotoxicity. Nat. Toxins. 3:1995;78-86.
20. Nagata S., Tsutsumi T., Hasegawa A., Yoshida F., Ueno Y. Enzyme immunoassay for direct determination of microcystins in environmental water. J. AOAC Int. 80:1997;408-417.
21. Newman D.J., Price C.P. Assessment and selection of antibodies. Price C.P., Newman D.J. Principles and Practice of Immunoassay. Second ed. 1997;123-138 MacMillan Reference, London, UK.
22. Pouria T., De Andrade A., Barbosa J., Cavalcanti R.L., Barreto V.S.T., Ward C.J., et al. Fatal microcystin intoxication in haemodialysis unit in Caruaru, Brazil. Lancet. 352:1998;26-31.
23. Sano T., Beattie K.A., Codd G.A., Kaya K. Two (Z)-Dehydrobutyrine-containing microcystins from a hepatotoxic bloom of Oscillatoria agardhii from Soulseat Loch, Scotland. J. Nat. Prod. 61:1998;851-853.
24. Sivonen K., Jones G.J. Cyanobacterial toxins. Chorus I., Bartram J. Toxic Cyanobacteria in Water. A Guide to their Public Health Consequences, Monitoring and Management. 1999;41-111 E. and F. N. Spon, London, UK.
25. Stanier R.Y., Kunisawa R., Mandel M., Cohen-Bazire G. Purification and properties of unicellular blue-green algae (order Chroococcales). Bacteriol. Rev. 35:1971;171-205.
26. Ward C.J., Beattie K.A., Lee E.Y.C., Codd G.A. Colorimetric protein phosphatase inhibition assay of laboratory strains and natural blooms of cyanobacteria: comparisons with high performance liquid chromatographic analysis for microcystins. FEMS Microbiol. Lett. 153:1997;465-473.
27. WHO (1997) Report of the Working Group on Chemical Substances in Drinking Water, Geneva, 22-26 April 1997. Section 5-2 Microcystin-LR. WHO, Geneva, Switzerland.