Expression, purification and characterization of recombinant human choline acetyltransferase: Phosphorylation of the enzyme regulates catalytic activity

Biochemical Journal

Volumn 349, Issue 1, 2000, Pages 141-151

  Dobransky, Tomas ^a,b   Davis, Wanda L ^a,b   Xiao, Gong Hua ^a,b,c   Rylett, R Jane ^a,b  

^a UNIVERSITY OF WESTERN ONTARIO   (Canada)

^b ROBARTS RESEARCH INSTITUTE   (Canada)

^c UNIVERSITY OF LOUISVILLE   (United States)

Author keywords

Acetylcholine; Cholinergic; Protein isoform

Indexed keywords

ACETYLCHOLINE; ALKALINE PHOSPHATASE; CASEIN KINASE II; CHOLINE ACETYLTRANSFERASE; CYCLIC AMP DEPENDENT PROTEIN KINASE; PROTEIN KINASE (CALCIUM,CALMODULIN) II; PROTEIN KINASE C;

ANIMAL CELL; ARTICLE; BACULOVIRUS; CELL MEMBRANE; CELL NUCLEUS; CELLULAR DISTRIBUTION; CYTOPLASM; DEPHOSPHORYLATION; ENZYME ACTIVITY; ENZYME PHOSPHORYLATION; ENZYME PURIFICATION; ESCHERICHIA COLI; GENE EXPRESSION; GENE EXPRESSION SYSTEM; IMMUNOFLUORESCENCE; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL;

ANIMALS; BACULOVIRIDAE; BLOTTING, WESTERN; CA(2+)-CALMODULIN DEPENDENT PROTEIN KINASE; CASEIN KINASE II; CATALYSIS; CELL LINE; CELL MEMBRANE; CHOLINE O-ACETYLTRANSFERASE; CHROMATOGRAPHY, AGAROSE; CYTOPLASM; ELECTROPHORESIS, GEL, TWO-DIMENSIONAL; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; HUMANS; IMMUNOHISTOCHEMISTRY; INSECTS; MICROSCOPY, CONFOCAL; MICROSCOPY, FLUORESCENCE; PHOSPHORYLATION; PROTEIN ISOFORMS; PROTEIN KINASE C; PROTEIN-SERINE-THREONINE KINASES; RECOMBINANT FUSION PROTEINS; RECOMBINANT PROTEINS; TIME FACTORS;

ANIMALIA; BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; INSECTA; UNIDENTIFIED BACULOVIRUS;

EID: 0034234806     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/0264-6021:3490141     Document Type: Article

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