Immobilization of α-chymotrypsin for use in batch and continuous reactors

Journal of Chemical Technology and Biotechnology

Volumn 75, Issue 7, 2000, Pages 519-525

  Mohapatra, Satish C ^a   Hsu, James T ^a  

^a Lehigh University   (United States)

Author keywords

Alginate gel; Continuous reactor; Enzyme activity; Enzyme leakage; Enzyme modification; Polyethylene glycol (PEG)

Indexed keywords

BIOREACTORS; CHEMICAL MODIFICATION; COMPUTER SIMULATION; DIFFUSION; ENZYME KINETICS; ENZYMES; GELS; HYDROLYSIS; POLYETHYLENE GLYCOLS;

ACETYL PHENYLALANINE METHYL ESTER; ALGINATE GEL; BATCH REACTOR; CHYMOTRYPSIN; CONTINUOUS REACTOR; DISTRIBUTION COEFFICIENT; ENZYME ACTIVITY; ENZYME MODIFICATION; GEL ENTRAPMENT;

ENZYME IMMOBILIZATION;

CALCIUM ALGINATE; CHYMOTRYPSIN A; MACROGOL; PHENYLALANINE METHYL ESTER;

BATCH PROCESS; BIOTECHNOLOGY;

ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; BIOMEDICAL TECHNOLOGY ASSESSMENT; CATALYSIS; ENZYME ACTIVITY; ENZYME MODIFICATION; HYDROLYSIS; NONHUMAN; PANCREAS; PROTEIN IMMOBILIZATION; REACTOR;

ANIMALIA; BOVINAE;

EID: 0034234589     PISSN: 02682575     EISSN: None     Source Type: Journal    
DOI: 10.1002/1097-4660(200007)75:7<519::AID-JCTB262>3.0.CO;2-N     Document Type: Article

References (19)

1. St Clair NL and Navia MA, Cross-linked enzyme crystals as robust biocatalysts. J Am Chem Soc 114:7314-7317 (1992).
2. Mohapatra SC and Hsu JT, Time dependent behaviour of the cross-linking reaction of α-chymotrypsin with glutaraldehyde. Biotechnol Tech 8:13-16 (1994).
3. Veronese FM, Largajolli R, Boccu E, Benassi CA and Schiavon O, Surface modification of the proteins. Activation of monomethoxy-polyethylene glycols by phenylchloroformates and modification of ribonuclease and superoxide dismutase. Appl Biochem Biotechnol 11:141-152 (1985).
4. Veronese FM, Calicetti P, Pastorino A, Schiavon O, Sartore L, Banci L and Scolaro LM, Preparation, physico-chemical and pharmacokinetic characterization of monomethoxypoly(ethylene glycol)-derivatized superoxide dismutase. J Controlled Release 10:145-154 (1989).
5. Fortier G and Laliberte M, Surface modification of horseradish peroxidase with poly(ethylene glycol)s of various molecular masses. Biotechnol Appl Biochem 17:115-130 (1993).
6. Kodera Y, Nishimura H, Matsushima A, Hiroto M and Inada Y, Lipase made active in hydrophobic media by coupling with polyethylene glycol. J Am Oil Chem Soc 71:335-338 (1994).
7. Mohapatra SC and Hsu JT, Optimizing lipase activity, enantioselectivity and stability with medium engineering and immobilization. Biotechnol Bioeng 64:213-220 (1999).
8. D'Urso EM and Fortier G, New hydrogel based on polyethylene glycol cross-linked with bovine serum albumin. Biotechnol Tech 8:71-76 (1994).
9. Ito Y, Fujii H and Imanishi Y, Modification of lipase with various synthetic polymers and their catalytic activities in organic solvents. Biotechnol Prog 10:398-402 (1994).
10. Cheetam PSJ, Blunt KW and Bucke C, Physical studies on cell immobilization using calcium-alginate gels. Biotechnol Bioeng 21:2155-2168 (1979).
11. Tanaka H, Matsumura M and Veliky IA, Diffusion characteristics of substrates in Ca-alginate beads. Biotechnol Bioeng 26:53-58 (1984).
12. Wheatley MA, Chang M, Park E and Langer R, Coated alginate microspheres: factors influencing the controlled delivery of macromolecules. J Appl Polym Sci 43:2123-2135 (1991).
13. Oyaas J, Storro I, Lysberg M, Svendsen H and Levine DW, The effective diffusion coefficient and the distribution constant for small molecules in calcium-alginate gel beads. Biotechnol Bioeng 47:492-500 (1995).
14. Oyaas J, Storro I, Lysberg M, Svendsen H and Levine DW, Determination of effective diffusion coefficients and distribution constants in polysaccharide gels with non-steadystate measurements. Biotechnol Bioeng 47:501-507 (1995).
15. Westrin BA and Axelsson A, Diffusion in gels containing immobilized cells: a critical review. Biotechnol Bioeng 38:439-446 (1991).
16. Handrikova G, Stefuca V, Polakovic S and Bales V, Determination of effective diffusion coefficient of substrate in gel particles with immobilized biocatalysis. Enz Microb Technol 18:581-584 (1996).
17. Stocks SJ, Jones AJM, Ramey CW and Brooks DE, A fluorimetric assay of the degree of modification of protein primary amines with polyethylene glycol. Anal Biochem 154:232-234 (1986).
18. Finlayson BA, Non-linear Analysis in Chemical Engineering, McGraw Hill, New York (1980).
19. Longo MA and Combes D, Thermostability of modified enzymes: a detailed study. J Chem Technol Biotechnol 74:25-32 (1999).