Production of α-Galactosyl epitopes via combined use of two recombinant whole cells harboring UDP-galactose 4-epimerase and α-1,3-galactosyltransferase

Biotechnology Progress

Volumn 16, Issue 4, 2000, Pages 595-599

  Chen, Xi ^a   Zhang, Wei ^a   Wang, Jianqiang ^a   Fang, Jianwen ^a   Wang, Peng George ^a  

^a WAYNE STATE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

EPIMERASE; GALACTOSE; GALACTOSYL EPITOPES; GALACTOSYLTRANSFERASE; LACTOSE;

ALPHA 1,3 GALACTOSYLTRANSFERASE; ALPHA GALACTOSYL EPITOPE; CARBOHYDRATE SYNTHESIS; EPITOPE; ESCHERICHIA COLI; GLUCOSYLTRANSFERASE; LACTOSYL AZIDE; OLIGOSACCHARIDE; ORGAN TRANSPLANTATION; REACTION MECHANISM; URIDINE DIPHOSPHATE GLUCOSE 4 EPIMERASE; XENOTRANSPLANTATION;

BIOSYNTHESIS; CATALYSIS; ENZYMES; ESCHERICHIA COLI; LIVING SYSTEMS STUDIES; SUGARS; TRANSPLANTS;

POLYSACCHARIDES;

CARBOHYDRATE SEQUENCE; CULTURE MEDIA; EPITOPES; ESCHERICHIA COLI; GALACTOSE; GALACTOSYLTRANSFERASES; HYDROGEN-ION CONCENTRATION; MOLECULAR SEQUENCE DATA; PLASMIDS; RECOMBINATION, GENETIC; UDPGLUCOSE 4-EPIMERASE;

BOVINAE; ESCHERICHIA COLI; PRIMATES; SUS SCROFA;

EID: 0034233443     PISSN: 87567938     EISSN: None     Source Type: Journal    
DOI: 10.1021/bp000052s     Document Type: Article

References (19)

1. Galili, U.; Swanson, K. Gene Sequences Suggest Inactivation of Alpha-1,3-Galactosyltransferase in Catarrhines After the Divergence of Apes from Monkeys. Proc. Natl. Acad. Sci. U.S.A. 1991, 88, 7401-7404.
2. Xu, Y.; Lorf, T.; Sablinski, T.; Ginaello, P.; Bailin, M.; Monroy, R.; Kozlowski, T.; Cooper, D. K. C.; Sachs, D. H. Removal of Anti-porcine Natural Antibodies from Human and Nonhuman Primate Plasma in vitro and in vivo by a Galα1-3Galβ1-4βGlc-X Immunoaffinity Column. Transplantation 1998, 65, 172-179.
3. Simon, P.; Neethling, F. A.; Taniguchi, S.; Good, P. L.; Zopf, D.; Hancock, W. W.; Cooper, D. K. C. Intravenous Infusion of α-Gal Oligosaccharides in Baboons Delays Hyperacute Rejection of Porcine Heart Xenografts. Transplantation 1998, 65, 246-353.
4. Nilsson, K. G. I. Glycosidase-catalysed Synthesis of Di- and Trisaccharide Derivatives Related to Antigens Involved in the Hyperacute Rejection of Xenotransplants. Tetrahedron Lett. 1997, 38, 133-136.
5. Fang, J. W.; Li, J.; Chen, X.; Zhang, Y. N.; Wang, J. Q.; Guo, Z. M.; Zhang, W.; Yu, L. B.; Brew, K.; Wang, P. G. Highly Efficient Chemoenzymatic Synthesis of α-Galactosyl Epitopes with a Recombinant α(1→3)-Galactosyltransferase. J. Am. Chem. Soc. 1998, 120, 6635-6638.
6. Galili, U. Interaction of the Natural Anti-Gal Antibody with α-Galactosyl Epitopes: A Major Obstacle for Xenotransplantation in Humans. Immunol. Today 1993, 14, 480-482.
7. Chen, X.; Kowal, P.; Hamad, S.; Fang, H.-N., Wang, P. G. Cloning, Expression and Characterization of a UDP-galactose 4-epimerase from E. coli. Biotechnol. Lett. 1999, 21, 1131-1135.
8. Herrmann, G. F.; Wang, P.; Shen, G.-J.; Wong, C.-H. Recombinant Whole Cells as Catalysts for the Enzymatic Synthesis of Oligosaccharides and Glycopeptides. Angew. Chem., Int. Ed. Engl. 1994, 33, 1241-1242.
9. Koizumi, S.; Endo, T.; Tabata, K.; Ozaki, A. Large-scale Production of UDP-galactose and Globotriose by Coupling Metabolically Engineered Bacteria. Nat. Biotechnol. 1998, 16, 847-850.
10. Endo, T.; Koizumi, S.; Tabata, K.; Kakita, S.; Ozaki, A. Large-scale Production of N-Acetyllactosamine through Bacterial Coupling. Carbohydr. Res. 1999, 316, 179-183.
11. Endo, T.; Koizumi, S.; Tabata, K.; Ozaki, A. Large-scale Production of CMP-NeuAc and Sialylated Oligosaccharides through Bacterial Coupling. Appl. Microbiol. Biotechnol. 2000, 53, 257-261.
12. Tabata, K.; Koizumi, S.; Endo, T.; Ozaki, A. Production of UDP-N-acetylglucosamine by Coupling Metabolically Engineered Bacteria. Biotech. Lett. 2000, 22, 479-483.
13. Zhang, W.; Wang, J.-Q.; Li, J.; Yu, L.-B.; Wang, P. G. A Large-scale Synthesis of an α-Galactosyl Trisaccharide Epitope Involved in the Hyperacute Rejection upon Xenotransplantation. J. Carbohydr. Chem. 1999, 18, 1009-1017.
14. Blanken, W. M.; Van den Eijnden, D. H. Biosynthesis of Terminal Galα1→3Galβ1→4GlcNAc-R Oligosaccharide Sequences on Glycoconjugates. J. Biol. Chem. 1985, 260, 12927-12934.
15. Metaye, T.; Mettey, Y.; Lehuede, J.; Vierfond, J. M.; Lalegerie, P. Comparative Inhibition of Human Alkaline Phosphatase and Diamine Oxidase by Bromo-levamisole, Cimetidine and Various Derivatives. Biochem. Pharmacol. 1988, 37, 4263-4268.
16. Martina, J. A.; Daniotti, J. L.; Maccioni, H. J. A UDP-sugar Pyrophosphatase Is Developmentally Regulated in the Rat Retina. J. Neurochem. 1995, 64, 1274-1280.
17. Martina, J. A.; Maccioni, H. J. UDP-sugar Pyrophosphatase of Rat Retina: Subcellular Localization and Topography. J. Neurosci. Res. 1996, 46, 485-491.
18. Paulson, J. C.; Rearick, J. I., Hill, R. L. Enzymatic Properties of β-D-Galactoside α2 Leads to 6-Sialyltransferase from Bovine Colostrum. J. Biol. Chem. 1977, 252, 2363-2371.
19. Weinstein, J.; De Souza-e-Silva, U.; Paulson, J. C. Sialylation of Glycoprotein Oligosaccharides N-linked to Asparagine. Enzymatic Characterization of a Gal β1→3(4)GlcNAc α2→3 Sialyltransferase and a Gal β1→4GlcNAc α2→6 Sialyltransferase from Rat Liver. J. Biol. Chem. 1982, 257, 13845-13853.