Diffusion bias and photophysical dynamics of single molecules in unsupported lipid bilayer membranes probed with confocal microscopy

Journal of Physical Chemistry B

Volumn 104, Issue 26, 2000, Pages 6103-6107

  Burden, Daniel L ^a   Kasianowicz, John J ^a  

^a NATIONAL INSTITUTE OF STANDARDS AND TECHNOLOGY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DIFFUSION; FLUORESCENCE; LIPIDS; MICROSCOPIC EXAMINATION; MOLECULAR DYNAMICS;

OPTICAL TRAPS; POLARIZABILITY; SCANNING CONFOCAL FLUORESCENCE MICROSCOPY;

MOLECULES;

EID: 0034225882     PISSN: 15206106     EISSN: None     Source Type: Journal    
DOI: 10.1021/jp0008546     Document Type: Article

References (26)

1. Keller, R. A.; Ambrose, W. P.; Goodwin, P. M.; Jet, J. H.; Martin, J. C.; Wu, M. Appl. Spectrosc. 1996, 50 (7), A12-A32.
2. Nie, S.; Zare, R. N. Annu. Rev. Biophys. Biomol. Struct. 1997, 26, 567.
3. Xie, X. S.; Trautmann, J. K. Annu. Rev. Phys. Chem. 1998, 49, 441.
4. Schmidt, Th.; Schutz, G.; Baumgartner, W.; Gruber, H.; Schindler, H. J. Phys. Chem. 1995, 99, 17662.
5. Sonnleitner, A.; Schutz, G.; Schmidt, Th. Biophys. J. 1999, 77, 2638.
6. Ide, T.; Yanagida, T. Biochem. Biophys. Res. Commun. 1999, 265, 595.
7. Ashkin, A.; Dziedzic, J. M.; Bjorkholm, J. E.; Chu, S. Opt. Lett. 1986, 11, 288.
8. Chiu, D.; Zare, R. N. J. Am. Chem. Soc. 1996, 118, 6512.
9. Osborne, M.; Balasubramanian, S.; Furey, W.; Klenerman, D. J. Phys. Chem. B 1998, 102, 3160.
10. Bar-Ziv, R.; Menes, R.; et al. Phys. Rev. Lett., 1995, 75, 3356.
11. o is the area of a molecule's head. Summing over the region illuminated by 50 mW gives an interaction energy of ≈0.65 eV, or ≈25kT. For the optical power range used in the present work (0.04-1.3 mW), an interaction energy of 0.02-0.65kT is calculated.
12. Bar-Ziv, R.; Moses, E.; Nelson, P. Biophys. J. 1998, 75, 294.
13. Schonle, A.; Hell, S. Opt. Lett. 1998, 23, 325.
14. Mueller, P.; Rudin, D.; Tien, H.; Wescott, W. C. Nature 1962, 194, 979.
15. Kasianowicz, J.; Bezrukov, S. Biophys. J. 1995, 69, 94.
16. 2/s for R6G in water
17. [Rigler, R.; et al. Eur. Biophys. J. 1993, 22, 169], the nonlinear least-squares fit produced an average value of ω = 264 ± 14 nm.
18. Nie, S.; Chiu, D.; Zare, R. N. Anal. Chem. 1995, 67, 2849.
19. Fahey, P. F.; Koppel, D. E.; Barak, L. S.; Wolf, D. E.; Elson, E. L.; Webb, W. W. Science 1977, 795, 305.
20. Soper, S.; Nutter, H.; Keller, R.; Davis, L.; Shera, E. Photochem. Photobiol. 1993, 57, 972.
21. It is possible that a light-induced single-molecule blinking phenomenon may also contribute to deviation away from expected behavior. Emission fluctuations on the time scale of milliseconds to seconds have been reported for a few immobilized fluorescent compounds and are thought to be the result of dynamic alterations in conformation [Weston, K.; Buratto, S. J. Phys. Chem A 1998, 102, 3635]. However, we observe similar interburst-interval curves for both headgroup labeled TRITC and C-12 labeled BODIPY lipids. Given the gross structural differences between the two molecules, it is unlikely that both exhibit comparable blinking characteristics. Furthermore, the simulation data of Figure 3A demonstrate that the onset of deviation away from Poisson prediction is consistent with the time scale of translational motion, as characterized by the measured D.
22. Kramers, H. A. Physica 1940, 7, 284.
23. Simon, A.; Libchaber, A. Phys. Rev. Lett. 1992, 68, 3375.
24. Xu, Z.; He, G.; Elking, M. J. Chem. Phys. 1997, 107 (10), 3947.
25. Pawley, J., Ed. Handbook of Biological Confocal Microscopy; Plenum Press: New York, 1990.
26. Schwille, P.; Korlach, J.; Webb, W. Cytometry 1999, 36, 176.