Use of a two-step Percoll® gradient for separation of loggerhead sea turtle peripheral blood mononuclear cells

Journal of Wildlife Diseases

Volumn 36, Issue 3, 2000, Pages 535-540

  Harms, Craig A ^a   Keller, Jennifer M ^b   Kennedy Stoskopf, Suzanne ^a  

^a NORTH CAROLINA STATE UNIVERSITY   (United States)

^b DUKE UNIVERSITY MARINE LABORATORY   (United States)

Author keywords

Caretta caretta; Density gradient centrifugation; Loggerhead turtle; Peripheral blood mononuclear cells

Indexed keywords

CARETTA CARETTA; CHELONIIDAE; TESTUDINES;

ANTICOAGULANT AGENT; CITRATE SODIUM; CITRIC ACID; EDETIC ACID; HEPARIN;

ANIMAL; ANIMAL DISEASE; ARTICLE; BLOOD; CELL SEPARATION; CELL SURVIVAL; CYTOLOGY; DENSITY GRADIENT CENTRIFUGATION; LABORATORY DIAGNOSIS; LEUKOCYTE COUNT; METHODOLOGY; MONONUCLEAR CELL; OSMOLARITY; REFERENCE VALUE; TURTLE;

ANIMALS; ANTICOAGULANTS; CELL SEPARATION; CELL SURVIVAL; CENTRIFUGATION, DENSITY GRADIENT; CITRATES; EDETIC ACID; HEPARIN; LEUKOCYTE COUNT; LEUKOCYTES, MONONUCLEAR; OSMOLAR CONCENTRATION; REFERENCE VALUES; SPECIMEN HANDLING; TURTLES;

EID: 0034219080     PISSN: 00903558     EISSN: None     Source Type: Journal    
DOI: 10.7589/0090-3558-36.3.535     Document Type: Article

References (19)

1. CAMPBELL, T. W. 1996a. Clinical pathology. In Reptile medicine and surgery, D. R. Mader (ed.). Saunders, Philadelphia, Pennsylvania, pp. 248-257.
2. _. 1996b. Sea turtle rehabilitation. In Reptile medicine and surgery, D. R. Mader (ed.). Saunders, Philadelphia, Pennsylvania, pp. 427-436.
3. CANNON, M. S. 1992. The morphology and cytochemistry of the blood leukocytes of Kemp's ridley sea turtle (Lepidochelys kempi). Canadian Journal of Zoology 70: 1336-1340.
4. COLIGAN, J. E., A. M. KRUISBEEK, D. H. MARGULIES, E. M. SHEVACH, AND W. STROBER. 1992. Current protocols in immunology, Vol. 1. Greene Publishing Associates and Wiley-Interscience, New York, New York, pp 7.1.1-7.24.4.
5. GEORGE, R. H. 1997. Health problems and diseases of sea turtles. In The biology of sea turtle, P. L. Lutz and J. A. Musick (eds.). CRC Press, Boca Raton, Florida, pp. 363-385.
6. HAWKEY, C. M., AND T. B. DENNETT. 1989. Color atlas of comparative veterinary hematology. Iowa State University Press, Ames, Iowa, 192 pp.
7. HERBST, L. H. 1994. Fibropapillomatosis of marine turtles. Annual Review of Fish Diseases 4: 389-425.
8. _, E. C. GREINER, L. M. EHRHART, D. A. BAGLEY, AND P. A. KLEIN. 1998. Serological association between spirorchidiasis, herpesvirus infection, and fibropapillomatosis in green turtles from Florida. Journal of Wildlife Diseases 34: 496-507.
9. LUTCAVAGE, M. E., P. PLOTKIN, B. WITHERINGTON, AND P. L. LUTZ. 1997. Human impacts on sea turtle survival. In The biology of sea turtles, P. L. Lutz and J. A. Musick (eds.). CRC Press, Boca Raton, Florida, pp. 387-409.
10. LUTZ, P. L. 1997. Salt, water, and pH balance in the sea turtle. In The biology of sea turtles, P. L. Lutz and J. A. Musick (eds.). CRC Press, Boca Raton, Florida, pp. 343-361.
11. MCKINNEY, E. C., AND T. B. BENTLEY. 1985. Cell-mediated immune response of Chelonia mydas. Developmental and comparative immunology 9: 445-452.
12. PHARMACIA BIOTECH. 1995. Percoll® Methodology and Applications, 2nd Edition. Revision 2. Pharmacia Biotech, Inc., Uppsala, Sweden, 59 pp.
13. PRITCHARD, P. C. H. 1997. Evolution, phylogeny, and current status. In The biology of sea turtles, P. L. Lutz and J. A. Musick (eds.). CRC Press, Boca Raton, Florida, pp. 1-28.
14. SAMOUR, J. H., J. C. HOWLETT, C. SILVANOSE, C. R. HASBUN, AND S. M. AL-GHAIS. 1998. Normal haematology of free-living green sea turtles (Chelonia mydas) from the United Arab Emirates. Comparative Hematology International 8: 102-107.
15. SMITH, W. L., L. A. TELL, M. B. KABBUR, L. GAGE, AND J. S. CULLOR. 1998. A rapid isolation of Asian elephant (Elephas maximus) blood heterophils on Percoll density gradients. Comparative Hematology International 8: 37-42.
16. TELL, L. A., M. B. KABBUR, W. L. SMITH, K. H. DAHL, AND J. S. CULLOR. 1998. A technique for isolating heterophils from blood of orange-winged Amazon parrots (Amazona amazonica amazonica). Comparative Hematology International 7: 47-53.
17. WATERSTRAT, P. R., A. J. AINSWORTH, AND G. CAPLEY. 1988. Use of a discontinuous Percoll gradient technique for the separation of channel catfish, Ictalurus punctatus (Rafinesque), peripheral blood leucocytes. Journal of Fish Diseases 11: 289-294.
18. WOOD, F. E., AND G. K. EBANKS. 1984. Blood cytology and hematology of the green sea turtle, Chelonia mydas. Herpetologica 40: 331-336.
19. WORK, T. M., R. E. RASKIN, G. H. BALAZS, AND S. D. WHITTAKER. 1998. Morphologic and cytochemical characteristics of blood cells from Hawaiian green turtles. American Journal of Veterinary Research 59: 1252-1257.