Expression of an active form of recombinant Ty1 reverse transcriptase in Escherichia coli: A fusion protein containing the C-terminal region of the Ty1 integrase linked to the reverse transcriptase-RNase H domain exhibits polymerase and RNase H activities

Biochemical Journal

Volumn 348, Issue 2, 2000, Pages 337-342

  Wilhelm, Marcelle ^a   Boutabout, Mansour ^a   Wilhelm, François Xavier ^a  

^a INST DE BIOL MOLEC ET CELLULAIRE   (France)

Author keywords

Polymerization; Retrotransposon; Sequence comparison; Template primer; Yeast

Indexed keywords

2',3' DIDEOXYNUCLEOSIDE DERIVATIVE; GUANOSINE TRIPHOSPHATE; HYBRID PROTEIN; INTEGRASE; MAGNESIUM CHLORIDE; RECOMBINANT PROTEIN; RIBONUCLEASE H; RNA DIRECTED DNA POLYMERASE; SODIUM CHLORIDE;

AMINO TERMINAL SEQUENCE; ARTICLE; CARBOXY TERMINAL SEQUENCE; DNA SYNTHESIS; ENZYME ACTIVITY; ESCHERICHIA COLI; GENETIC RECOMBINATION; NONHUMAN; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN FOLDING; PROTEIN PURIFICATION; RETROPOSON;

AMINO ACID SEQUENCE; CHROMATOGRAPHY, AFFINITY; CLONING, MOLECULAR; CODON, TERMINATOR; DNA; ESCHERICHIA COLI; HIV-1 REVERSE TRANSCRIPTASE; INTEGRASES; KINETICS; MOLECULAR SEQUENCE DATA; NUCLEIC ACID HETERODUPLEXES; OPEN READING FRAMES; RECOMBINANT FUSION PROTEINS; RETROELEMENTS; RIBONUCLEASE H, CALF THYMUS; RNA; RNA-DIRECTED DNA POLYMERASE; SACCHAROMYCES CEREVISIAE; SEQUENCE ALIGNMENT; SEQUENCE HOMOLOGY, AMINO ACID; TEMPLATES, GENETIC;

ESCHERICHIA COLI; SACCHAROMYCES; SACCHAROMYCES CERVISIAE;

EID: 0034214101     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/0264-6021:3480337     Document Type: Article

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