Effects of triglyceride accumulation on induction of urea synthesis by glucagon and dexamethasone in monolayer cultures of bovine hepatocytes

Journal of Animal Science

Volumn 78, Issue 6, 2000, Pages 1659-1666

  Zhu, L H ^a   Strang, B D ^a   Armentano, L E ^a  

^a UNIVERSITY OF WISCONSIN MADISON   (United States)

Author keywords

Bovidae; Dexamethasone; Fatty Liver; Glucagon; Liver Cells; Urea Biosynthesis

Indexed keywords

BOVIDAE; BOVINAE;

AMMONIUM CHLORIDE; ARGINASE; DEXAMETHASONE; FATTY ACID; GLUCAGON; ORNITHINE CARBAMOYLTRANSFERASE; TRIACYLGLYCEROL; UREA;

AGING; ANIMAL; ARTICLE; CATTLE; CELL CULTURE; DRUG EFFECT; LIVER; METABOLISM;

AGING; AMMONIUM CHLORIDE; ANIMALS; ARGINASE; CATTLE; CELLS, CULTURED; DEXAMETHASONE; FATTY ACIDS, NONESTERIFIED; GLUCAGON; LIVER; ORNITHINE CARBAMOYLTRANSFERASE; TRIGLYCERIDES; UREA;

EID: 0034202808     PISSN: 00218812     EISSN: None     Source Type: Journal    
DOI: 10.2527/2000.7861659x     Document Type: Article

References (41)

1. Bauchart, D., D. Durand, C. Picherit, B. Graulet, and D. Gruffat. 1998. Effects of dietary coconut oil on blood transport and in vivo hepatic metabolism of fatty acids in the preruminant calf. Reprod. Nutr. Develop. 38:203-204.
2. Bell, A. W. 1995. Regulation of organic nutrient metabolism during transition from late pregnancy to early lactation. J. Anim. Sci. 73:2804-2819.
3. Bergman, E. N. 1973. Glucose metabolism in ruminants as related to hypoglycemia and ketosis. Cornell Vet. 63:341-382.
4. Bergman, E. N., R. P. Brochman, and C. F. Kaufman. 1974. Glucose metabolism in ruminants: Comparison of whole-body turnover with production by gut, liver, and kidneys. Fed. Proc. 33:1849-1854.
5. Bergman, E. N., and R. N. Heitmann. 1978. Metabolism of amino acids by the gut, liver, kidneys, and peripheral tissues. Fed. Proc. 37:1228-1232.
6. Bjornsson, O. G., J. M. Duerden, S. M. Bartlett, J. D. Sparks, C. E. Sparks, and G. F. Gibbons. 1992. The role of pancreatic hormones in the regulation of lipid storage, oxidation and secretion in primary cultures of rat hepatocytes. Biochem. J. 281:381-386.
7. Brockman, R. P., E. N. Bergman, P. K. Joo, and J. G. Manns. 1975. Effects of glucagon and insulin on net hepatic metabolism of glucose precursors in sheep. Am. J. Physiol. 229:1344-1350.
8. Danno, H., Y. Jincho, S. Budiyanto, Y. Furukawa, and S. Kimura. 1992. A simple enzymatic quantitative analysis of triglycerides in tissues. J. Nutr. Sci. Vitaminol. 38:517-521.
9. Davidovich, A., E. E. Bartley, G. A. Milliken, A. D. Dayton, C. W. Deyoe, and R. M. Bechtle. 1977a. Ammonia toxicity in cattle. IV. Effects of unprocessed or extrusion-cooked mixtures of grain and urea, biuret, or dicyanodiamide and liquid supplements on rumen and blood changes associated with toxicity. J. Anim. Sci. 45:1397-1408.
10. Davidovich, A., T. E. Chapman, R. M. Bechtle, A. D. Dayton, and R. A. Frey. 1977b. Ammonia toxicity in cattle. II. Changes in carotid and jugular blood components associated with toxicity. J. Anim. Sci. 44:702-709.
11. Donkin, S. S., and L. E. Armentano. 1993. Preparation of extended in vitro cultures of bovine hepatocytes that are hormonally responsive. J. Anim. Sci. 71:2218-2227.
12. Donkin, S. S., and L. E. Armentano. 1994. Regulation of gluconeogenesis by insulin and glucagon in the neonatal bovine. Am. J. Physiol. 266:R1229-R1237.
13. Felipo, V., M. D. Minana, and S. Grisolia. 1988. Long-term ingestion of ammonium increases acetylglutamate and urea levels without affecting the amount of carbomoyl-phosphate synthase. Eur. J. Biochem. 176:567-571.
14. Folch, J., M. Lees, and G. H. Sloane Stanley. 1957. A simple method for isolation and purification of total lipides from animal tissues. J. Biol. Chem. 226:497-509.
15. Gaal, T., I. M. Reid, R. A. Collins, C. J. Roberts, and B. V. Pike. 1983. Comparison of biochemical and histological methods of estimating fat content of liver of dairy cows. Res. Vet. Sci. 34:245-248.
16. Gebhardt, R., and D. Mecke. 1979. Permissive effect of dexamethasone on glucagon induction of urea-cycle enzymes in perfused primary monolayer cultures of rat hepatocytes. Eur. J. Biochem. 97:29-35.
17. Grummer, R. R. 1993. Etiology of lipid related metabolic disorders in periparturient dairy cattle. J. Dairy Sci. 76:3882-3896.
18. Grummer, R. R. 1995. Impact of changes in organic nutrient metabolism on feeding the transition dairy cow. J. Anim. Sci. 73:2820-2833.
19. Heimberg, M., I. Weinstein, and M. Kohout. 1969. The effects of glucagon, dibutyryl cyclic adenosine 2′, 5′-monophosphate, and concentration of free fatty acid on hepatic lipid metabolism. J. Biol. Chem. 244:5131-5139.
20. Hippen A. R., P. She, J. W. Young, D. C. Beitz, G. L. Lindberg, L. F. Richardson, and R. W. Tucker. 1999. Alleviation of fatty liver in dairy cows with 14-day intravenous infusions of glucagon. J. Dairy Sci. 82:1139-1152.
21. Huizenga, J. R., C. H. Glips, and A. Tangerman. 1996. The contribution of various organs to ammonia formation: A review of factors determining the arterial concentration. Ann. Clin. Biochem. 33:23-30.
22. Jenkins K. J., and J. K. G. Kramer. 1986. Influence of low linoleic and linolenic acids in milk replacer on calf performance and lipids in blood plasma, heart, and liver. J. Dairy Sci. 69:1374-1386.
23. Joseph, S. K., and J. D. McGivan. 1978. The effect of ammonium chloride and glucagon on the metabolism of glutamine in isolated liver cells from starved rats. Biochim. Biophys. Acta 543:16-28.
24. Kaiser, S., W. Gerok, and D. Haussinger. 1988. Ammonia and glutamine metabolism in human liver slices: New aspects on the pathogenesis of hyperammonaemia in chronic liver disease. Eur. J. Clin. Investig. 18:535-542.
25. Kennedy, P. M., and L. P. Milligan. 1978. Transfer of urea from the blood to the rumen of sheep. Br. J. Nutr. 40:149-154.
26. LaBarca, C., and K. Paigen. 1980. A simple, rapid, and sensitive DNA assay procedure. Anal. Biochem. 102:344-352.
27. Lachin, J. M. 1981. Introduction on sample size determination and power analysis for clinical trials. Control. Clin. Trials 2:93-113.
28. Lin, R. C., P. J. Snodgrass, and D. Rabier. 1982. Induction of urea cycle enzymes by glucagon and dexamethasone in monolayer cultures of rat hepatocytes. J. Biol. Chem. 257:5061-5067.
29. Littell, R. C., G. A. Milliken, W. W. Stroup, and R. D. Wolfinger. 1996. SAS System for Mixed Models. SAS Inst. Inc., Cary, NC.
30. Maswoswe, S. M., and G. C. Tremblay. 1989. Biosynthesis of hippurate, urea and pyrimidines in the fatty liver: Studies with rats fed orotic acid or a diet deficient in choline and inositol, and with genetically obese (Zucker) rats. J. Nutr. 119:273-279.
31. Nebes, V. L., and S. M. Morris Jr. 1988. Regulation of messenger ribonucleic acid levels for five urea cycle enzymes in cultured rat hepatocytes. Requirements for cyclic adenosine monophosphate, glucocorticoids and ongoing protein synthesis. Mol. Endocrinol. 2:444-451.
32. Renouf, S., C. Buquet, A. Fairand, M. Benamar, and A. Husson. 1993. Changes in levels of argininosuccinate lyase mRNA during induction by glucagon and cyclic AMP in cultured foetal-rat hepatoctytes. Biochem. J. 291:609-613.
33. Reynolds, C. K., G. B. Huntington, H. F. Tyrell, and P. J. Reynolds. 1988. Net portal-drained visceral and hepatic metabolism of glucose, L-lactate, and nitrogenous compounds in lactating Holstein cows. J. Dairy Sci. 71:1803-1812.
34. SAS. 1989. SAS/STAT User's Guide. (Version 6, 4th Ed.). SAS Inst. Inc., Cary, NC.
35. Schimke, R. T. 1962. Adaptive characteristics of urea cycle enzymes in the rat. J. Biol. Chem. 237:459-468.
36. Snodgrass, P. J., R. C. Lin, W. A. Muller, and T. T. Aoki. 1978 Induction of urea cycle enzymes of rat liver by glucagon. J. Biol. Chem. 253:2748-2753.
37. Strang, B. D., S. J. Bertics, R. R. Grummer, and L. E. Armentano. 1998a. Effect of long-chain fatty acids on triglyceride accumulation, gluconeogenesis and ureagenesis in bovine hepatocytes. J. Dairy Sci. 81:728-739.
38. Strang, B. D., S. J. Bertics, R. R. Grummer, and L. E. Armentano. 1998b. Relationship of triglyceride accumulation to insulin clearance and hormonal responsiveness in bovine hepatocytes. J. Dairy Sci. 81:740-747.
39. Summerskill, W. H. J., and E. Wolpert. 1970. Ammonia metabolism in the gut. Am. J. Clin. Nutr. 23:633-639.
40. Weber, F. L., Jr., and G. L. Veach. 1979. The importance of the small intestine in gut ammonia production in the fasting dog. Gastroenterology 77:235-240.
41. Zhu, L. H., J. H. Eisemann, and L. E. Armentano. 1997. Direct chronic effects of β-agonists on gluconeogenesis and ureagenesis in monolayer cultures of bovine hepatocytes. J. Dairy Sci. 80:165 (Abstr.).