Identification and characterisation of the excreted/secreted serine proteases of larvae of the Old World Screwworm Fly, Chrysomya bezziana

International Journal for Parasitology

Volumn 30, Issue 6, 2000, Pages 705-714

  Muharsini, Sri ^d   Sukarsih, ^b   Riding, George ^a   Partoutomo, Sutijono ^b   Hamilton, Susan ^c   Willadsen, Peter ^a   Wijffels, Gene ^a  

^a CSIRO   (Australia)

^b Research Institute for Veterinary Science Bogor   (Indonesia)

^c UNIVERSITY OF QUEENSLAND   (Australia)

^d NONE

Author keywords

Chrysomya bezziana; Chymotrypsin; Excreted or secreted; Lucilia cuprina; Peritrophic matrix; Serine proteases; Trypsin

Indexed keywords

SERINE PROTEINASE;

AMINO ACID SEQUENCE; ARTICLE; CHRYSOMYA BEZZIANA; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME PURIFICATION; ENZYME SUBSTRATE; IMMUNOBLOTTING; LARVA; MYIASIS; NONHUMAN; PARASITE; PARASITE IMMUNITY; PH;

AMINO ACID SEQUENCE; ANIMALS; DIPTERA; ELECTROPHORESIS, POLYACRYLAMIDE GEL; LARVA; MOLECULAR SEQUENCE DATA; MOLECULAR WEIGHT; SERINE ENDOPEPTIDASES;

CHRYSOMYA BEZZIANA; GLYCINE MAX; INSECTA; INVERTEBRATA; LUCILIA CUPRINA;

EID: 0034194875     PISSN: 00207519     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0020-7519(00)00055-2     Document Type: Article

References (40)

1. Bowles V.M., Carnegie P.R., Sandeman R.M. Characterization of proteolytic and collagenolytic enzymes from the larvae of Lucilia cuprina, the sheep blow fly. Aust. J. Biol. Res. 41:1988;269-278.
2. Sandeman R.M., Feehan J.P., Chandler R.A., Bowles V.M. Tryptic and chymotryptic proteases released by larvae of the blow fly, Lucilia cuprina. Int. J. Parasitol. 20:1990;1019-1023.
3. Casu R.E., Jarmey J.M., Elvin C.M., Eisemann C.H. Isolation of a trypsin-like serine protease gene family from the sheep blow fly L. cuprina. Insect. Mol. Biol. 3:1994;159-170.
4. Bowles V.M., Feehan J.P., Sandeman R.M. Sheep plasma inhibitors influencing protease activity and growth of Lucilia cuprina larvae in vitro. Int. J. Parasitol. 20:1990;169-174.
5. Tellam R.L., Bowles V.M. Control of blowfly strike in sheep: current strategies and future prospects. Int. J. Parasitol. 27:1997;261-273.
6. Young A.R., Meeusen E.N.T., Bowles V.M. Characterization of ES products involved in wound initiation by Lucilia cuprina larvae. Int. J. Parasitol. 26:1996;245-252.
7. Young A.R., Meeusen E.N.T., Mancuso N., Bowles V.M. Proteases released by Lucilia cuprina during egg hatch. Insect. Biochem. Mol. Biol. 27:1997;1017-1026.
8. Tellam R.T., Eisemann C.H., Pearson R.D. Vaccination of sheep with purified serine proteases from the secretory and excretory material of Lucilia cuprina larvae. Int. J. Parasitol. 24:1994;757-764.
9. Casu R.E., Eisemann C.H., Vuocolo T., Tellam R.L. The major excretory/secretory protease from Lucilia cuprina larvae is also a gut digestive protease. Int. J. Parasitol. 26:1996;623-628.
10. Lecroisey A., Boulard C., Keil B. Chemical and enzymatic characterization of the collagenase from the insect Hypoderma lineatum. Eur. J. Biochem. 101:1979;385-393.
11. Tong N.T., Imhoff J.-M., Lecroisey A., Keil B., Hypodermin A. A trypsin-like neutral proteinase from the insect Hypoderma lineatum. Biochim. Biophys. Acta. 658:1981;209-219.
12. East I.J., Allingham P.G., Bunch R.J., Matheson J. Isolation and characterization of a trypsin-like enzyme from buffalo fly, Haematobia irritans exigua. Med. Vet. Entomol. 9:1995;120-126.
13. Houseman J.G., Campbell F.C., Morrison P.E. A preliminary characterization of digestive proteases in the posterior midgut of the stable fly Stomoxys calcitrans (L.) (Diptera: Muscidae). Insect Biochem. 17:1987;213-218.
14. Jordao B.P., Lehane M.J., Terra W.R., Ribeiro A.F., Ferreira C. An immunocytochemical investigation of trypsin secretion in the midgut of the stable fly, Stomoxys calcitrans. Insect Biochem. Mol. Biol. 26:1996;445-453.
15. Lehane S.M., Assinder S.J., Lehane M.J. Cloning, sequencing, temporal expression and the tissue-specificity of two serine proteases from the midgut of the blood-feeding fly Stomoxys calcitrans. Eur. J. Biochem. 254:1998;290-296.
16. Elvin C.M., Vuocolo T., Smith W.J.M., Eisemann C.H., Riddles P.W. An estimate of the number of serine proteases genes expressed in sheep blowfly larvae (Lucilia cuprina). Insect Mol. Biol. 3:1994;105-115.
17. Elvin C.M., Whan V., Riddles P.W. A family of serine protease genes expressed in adult buffalo fly (Haematobia irritans exigua). Mol. Gen. Genet. 240:1993;132-139.
18. Humprey J.D., Spradberry J.P., Tozer R.S. Chrysomya bezziana: Pathology of old world screw-worm fly infestation in cattle. Exp. Parasitol. 49:1980;381-397.
19. Norris K.R., Murray M.D. Notes on the screwworm fly Chrysomya bezziana (Diptera. Calliphoridae) as a pest of cattle in Papua New Guinea. CSIRO. Division of Entomology Technical Paper. 6:1964;1-26.
20. Spradberry J.P., Vanniasingham J.A. Incidence of the screwworm fly, Chrysomya bezziana, at the Zoo Negara, Malaysia. The Malaysian Vet. J. 7:1980;28-32.
21. Sigit S.H., Partoutomo S. Myasis in Indonesia. Bull. Int. Epiz. 93:1981;173-178.
22. Sukarsih S., Tozer R.S., Knox M.R. Collection and case incidence of the Old World screwworm fly, Chrysomya bezziana, in three localities in Indonesia. Penyakit. Hewan. 21:1989;114-117.
23. Navidpour S., Hoghooghi-rad N., Goodarzi H., Pooladgar A.R. Outbreak of Chrysomya bezziana in Khoozestan province, Iran. Vet. Rec. 139:1996;9.
24. East I.J., Fitzgerald C.J., Pearson R.D., et al. Lucilia cuprina: Inhibition of larval growth induced by immunization of host sheep with extracts of larval peritrophic membrane. Int. J. Parasitol. 23:1993;221-229.
25. Rabilloud T., Carpentier G., Tarroux P. Improvement and simplification of low background silver staining of proteins by using sodium dithionate. Electrophoresis. 9:1988;288-291.
26. Wijffels G., Fitzgerald C., Gough J., et al. The cloning and characterisation of angiotensin-converting enzyme from the dipteran species, Haematobia irritans exigua, and its expression in the maturing male reproductive system. Eur. J. Biochem. 273:1996;414-423.
27. Heussen C., Dowdle E.B. Electrophoretic analysis of plasminogen activators in polyacrylamide gels containing sodium dodecyl sulfate and copolymerized substrate. Anal. Biochem. 102:1980;196-202.
28. Geiger R, Fritz H. Trypsin. In: Bergmeyer HU, editor. Methods of enzymatic analysis, vol. 5. Weinheim: Verlag-Chemie, 1984. p. 119-29.
29. Geiger R. Chymotrypsin. In: Bergmeyer HU, editor. Methods of enzymatic analysis, vol. 5. Weinheim: Verlag-Chemie, 1984. p. 99-109.
30. Wijffels G., Gough J., Muharsini S., Donaldson A., Eisemann C. Expression of angiotensin-converting enzyme related carboxydipeptidases in the larvae of four species of fly. Insect Biochem. Mol. Biol. 27:1997;451-460.
31. Casu R.E., Pearson R.D., Jarmey J.M., Cadogan L.C., Riding G.A., Tellam R.L. Excretory/secretory chymotrypsin from L. cuprina: Purification, enzymatic specificity and amino acid sequence deduced from mRNA. Insect Mol. Biol. 3:1994;202-311.
32. Terra W.R., Ferreira C. Insect digestive enzymes: properties, compartmentalization and function. Comp. Biochem. Physiol. 109:1994;1-62.
33. Sasaki T., Suzuki Y. Alkaline proteases in the digestive juice of the silkworm, Bombyx mori. Biochim. Biophys. Acta. 703:1982;1-10.
34. Tsai I.-H., Chuang K.L., Chuang J.L. Chymotrypsins in digestive tracts of crustacean decapods (shrimp). Comp. Biochem. Physiol. 85B:1985;235-239.
35. Peterson A.M., Fernando G.J., Wells M.A. Purification, characterization and cDNA sequence of an alkaline chymotrypsin from the midgut of Manduca sexta. Insect Biochem. Mol. Biol. 25:1995;765-774.
36. Waterhouse D.F. Studies of the physiology and toxicology of blowflies. 5. The hydrogen ion concentration in the alimentary canal. CSIRO Pamphlet. 102:1940;7-27.
37. Hobson R.P. On an enzyme from blow-fly larvae (Lucilia sericata) which digests collagen in alkaline solution. Biochem. J. 25:1931;1458-1463.
38. Lemos F.J.A., Terra W.R. Soluble and membrane-bound forms of trypsin-like enzymes in Musca domestica larval midgut. Insect Biochem. Mol. Biol. 22:1992;613-619.
39. Jordao B.P., Terra W.R., Ribeiro F., Lehane M.J., Ferreira C. Trypsin secretion in Musca domestica larval midguts: A biochemical and immunocytochemical study. Insect Biochem. Mol. Biol. 26:1996;337-346.
40. Davis C.A., Riddell D.C., Higgins M.J., Holden J.J.A., White B.N. A gene family in Drosophila melanogaster coding for trypsin-like genes. Nucleic Acids Res. 13:1985;6605-6619.