Identification of a putative LPS-associated cation exporter from Rhizobium leguminosarum bv. viciae

FEMS Microbiology Letters

Volumn 186, Issue 1, 2000, Pages 47-53

  Allaway, D ^a   Calvaco, L ^a   Saini, S ^a   Hocking, P ^a   Lodwig, E M ^a   Leonard, M E ^a   Poole, P S ^a  

^a UNIVERSITY OF READING   (United Kingdom)

Author keywords

Antiporter; Calcium; Lipopolysaccharide

Indexed keywords

ANTIPORTER; LIPOPOLYSACCHARIDE;

AMINO ACID SEQUENCE; ARTICLE; CALCIUM TRANSPORT; CATION TRANSPORT; CONTROLLED STUDY; DNA DETERMINATION; DRUG STRUCTURE; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; RHIZOBIUM LEGUMINOSARUM; TRANSPOSON;

AMINO ACID SEQUENCE; BACTERIAL PROTEINS; CALCIUM; CALCIUM-BINDING PROTEINS; CULTURE MEDIA; DNA TRANSPOSABLE ELEMENTS; HEXOSYLTRANSFERASES; HYDROGEN-ION CONCENTRATION; LIPOPOLYSACCHARIDES; MAGNESIUM; MOLECULAR SEQUENCE DATA; PLASMIDS; RHIZOBIUM LEGUMINOSARUM; SEQUENCE ALIGNMENT; SEQUENCE ANALYSIS, DNA; SUGAR ACIDS; TRANSFERASES;

NEGIBACTERIA; RHIZOBIUM; RHIZOBIUM LEGUMINOSARUM; RHIZOBIUM LEGUMINOSARUM BV. VICIAE;

EID: 0034194598     PISSN: 03781097     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1097(00)00114-2     Document Type: Article

References (22)

1. Vincent J.M., Humphrey B.A. Partition of divalent cations between bacterial wall and cell contents. Nature. 199:1963;149-151.
2. Allaway D., Jeyaretnam B., Carlson R.W., Poole P.S. Genetic and chemical characterization of a mutant that disrupts synthesis of the lipopolysaccharide core tetrasaccharide in Rhizobium leguminosarum. J. Bacteriol. 178:1996;6403-6406.
3. Kadrmas J.L., Allaway D., Studholme R.E., Sullivan J.T., Ronson C.W., Poole P.S., Raetz C.R.H. Cloning and overexpression of glycosyltransferases that generate the lipopolysaccharide core of Rhizobium leguminosarum. J. Biol. Chem. 273:1998;26432-26440.
4. Poole P.S., Schofield N.A., Reid C.J., Drew E.M., Walshaw D.L. Identification of chromosomal genes located downstream of dctD that affect the requirement for calcium and the lipopolysaccharide layer of Rhizobium leguminosarum. Microbiology. 140:1994;2797-2809.
5. Beringer J.E. R factor transfer in Rhizobium leguminosarum. J. Gen. Microbiol. 84:1974;188-198.
6. Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989) Cold Spring Harbor Laboratory Press, New York.
7. Simon R., Quandt J., Klipp W. New derivatives of transposon Tn5 suitable for mobilization of replicons, generation of operon fusions and induction of genes in Gram-negative bacteria. Gene. 80:1989;161-169.
8. Ruvkun G.B., Ausubel F.M. A general method for site-directed mutagenesis in prokaryotes. Nature. 289:1981;85-88.
9. Thompson J.D., Higgins D.G., Gibson T.J. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 22:1994;4673-4680.
10. Poole P.S., Blyth A., Reid C.J., Walters K. myo-Inositol catabolism and catabolite regulation in Rhizobium leguminosarum bv. viciae. Microbiology. 140:1994;2787-2795.
11. + antiporter-deficient strains by the overexpressed gene. J. Biol. Chem. 268:1993;11296-11303.
12. Freiberg C., Fellay R., Bairoch A., Broughton W.J., Rosenthal A., Perret X. Molecular basis of symbiosis between Rhizobium and legumes. Nature. 387:1997;394-401.
13. Ohyama T., Igarashi K., Kobayashi H. Physiological-role of the chaA gene in sodium and calcium circulations at a high pH in Escherichia coli. J. Bacteriol. 176:1994;4311-4315.
14. 2+ exchanger. Science. 250:1990;562-565.
15. + antiporter coded by nhaA (Ant) from Escherichia coli. J. Biol. Chem. 266:1991;11289-11294.
16. Brey R.N., Rosen B.P. Properties of Escherichia coli mutants altered in calcium/proton antiport activity. J. Bacteriol. 139:1979;824-834.
17. Johnston A.W.B., Beringer J.E. Identification of the Rhizobium strains in pea root nodules using genetic markers. J. Gen. Microbiol. 87:1975;343-350.
18. Glenn A.R., Poole P.S., Hudman J.F. Succinate uptake by free-living and bacteroid forms of Rhizobium leguminosarum. J. Gen. Microbiol. 119:1980;267-271.
19. Figurski D.H., Helinski D.R. Replication of an origin-containing derivative of plasmid RK2 dependent on a plasmid function provided in trans. Proc. Natl. Acad. Sci. USA. 76:1979;1648-1652.
20. Mavridou A., Barny M.A., Poole P., Plaskitt K., Davies A.E., Johnston A.W.B., Downie J.A. Rhizobium leguminosarum nodulation gene (nod) expression is lowered by an allele specific mutation in the dicarboxylate transport gene dctB. Microbiology. 141:1995;103-111.
21. Kovach M.E., Elzer P.H., Hill D.S., Robertson G.T., Farris M.A., Roop J.M. II, Peterson K.M. Four new derivatives of the broad-host-range cloning vectors pBBR1MCS, carrying different antibiotic-resistance cassettes. Gene. 166:1995;175-176.
22. Hirsch P.R., Beringer J.E. A physical map of pPH1JI and pJB4JI. Plasmid. 12:1984;139-141.