Synthesis of peptidyl methylcoumarin esters as substrates and active- site titrants for the prohormone processing proteases Kex2 and PC2

Analytical Biochemistry

Volumn 280, Issue 2, 2000, Pages 201-208

  Rockwell, Nathan C ^a,b   Krysan, Damian J ^a   Fuller, Robert S ^a  

^a UNIVERSITY OF MICHIGAN MEDICAL SCHOOL   (United States)

^b STANFORD UNIVERSITY   (United States)

Author keywords

Active site titration; Kex2; PC2; Protease substrates; Serine protease

Indexed keywords

AMIDES; AMINO ACIDS; TITRATION; YEAST;

ACTIVE SITE; DEPROTECTION; KEX2; MODEL SUBSTRATES; POTENTIAL UTILITY; PRO-HORMONE PROCESSING; PROCESSING PROTEASE; SERINE PROTEASE;

ESTERS;

COUMARIN DERIVATIVE; PROTEINASE; SERINE PROTEINASE;

ARTICLE; ENZYME ACTIVE SITE; ENZYME ANALYSIS; ENZYME SPECIFICITY; NONHUMAN; PRIORITY JOURNAL; SACCHAROMYCES CEREVISIAE; STEADY STATE; SYNTHESIS; TITRIMETRY;

EID: 0034194479     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1006/abio.2000.4541     Document Type: Article

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