Detection of DNA using a visible dye, Nile blue, in electrophoresed gels

Analytical Biochemistry

Volumn 280, Issue 2, 2000, Pages 322-324

  Yang, Yong Il ^a   Hong, Hee Youn ^a   Lee, Ik Soo ^a   Bai, Dong Gyu ^a   Yoo, Gyurng Soo ^a   Choi, Jung Kap ^a  

^a Chonnam National University   (South Korea)

Author keywords

[No Author keywords available]

Indexed keywords

BIOCHEMISTRY; CHEMISTRY;

GELS;

AGAROSE; DYE; ETHIDIUM BROMIDE;

DNA DETERMINATION; GEL ELECTROPHORESIS; NONHUMAN; NOTE; PRIORITY JOURNAL; STAINING; ULTRAVIOLET IRRADIATION;

EID: 0034194391     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1006/abio.2000.4537     Document Type: Article

References (12)

1. Sharp P. A., Sugden B., Sambrook J. Detection of two restriction endonuclease activities in Haemophilius parainfluenza using analytical agarose. Biochemistry. 12:1973;3055-3062.
2. Kapuscinski J., Skoczylas B. Simple and rapid fluorometric method for DNA microassay. Anal. Biochem. 83:1977;252-257.
3. Cerutti P. A. Prooxidant states and rumor promotion. Science. 227:1985;375-381.
4. Keshava C., Ong T., Nath J. Comparative studies on radiation-induced micronuclei and chromosomal aberrations in V79 cells. Mutant Res. 328:1995;63-71.
5. Kantor G. J., Hull D. R. An effect of ultraviolet light on RNA and protein synthesis in nondividing human diploid fibroblasts. Biophys. J. 27:1979;359-370.
6. Daru Y. S., Ramesh K. Protocols for the visualization of DNA in electrophoretic gels by a safe and inexpensive alternative to ethidium bromide. Technique - J. Methods Cell Mol. Biol. 1:1989;183-187.
7. Adkins S., Burmeister M. Visualization of DNA in agarose gels as migrating colored bands; applications for preparative gels and educational demonstrations. Anal. Biochem. 240:1996;17-23.
8. Torres J. S., Noyala P. A novel stain for DNA in agarose gels. Tech. Tips. 9:1993;40.
9. Dutt M. K. Aqueous solutions of some basic dyes-Their use in the cytochemical detection of DNA. Folia Histochem. Cytochem. (Krakow). 18:1980;53-59.
10. Sambrook J., Fritsch E. F., Maniatis T. Molecular Cloning: A Laboratory Manual. 1989;Cold Spring Harbor Press, Cold Spring Harbor. p. 1.53-1.82.
11. Sinden R. R. DNA Structure and Function. 1994;Academic Press, San Diego. p. 109-110.
12. Kim N. S. Stain Chemistry. 1995;Kyomunsa, Seoul. p. 51-53.