Phage display and pharmacogenomics

Pharmacogenomics

Volumn 1, Issue 2, 2000, Pages 199-202

  Ladner, Robert Charles ^a  

^a Dyax Corp   (United States)

Author keywords

Binding agents; Phage display; Surface display; Synthetic diversity

Indexed keywords

BACTERIOPHAGE; GENETIC VARIABILITY; GENETICS; METHODOLOGY; PHARMACOGENETICS; REVIEW;

BACTERIOPHAGES; PHARMACOGENETICS; VARIATION (GENETICS);

EID: 0034189481     PISSN: 14622416     EISSN: None     Source Type: Journal    
DOI: 10.1517/14622416.1.2.199     Document Type: Review

References (10)

1. Phage Display of Peptides and Proteins. Kay BK, Winter J, McCafferty J (Eds.), Academic Press, San Diego, USA (1996). This book presents a thorough but concise discussion of many aspects of phage display.
2. DAUGHERTY PS, OLSEN MJ, IVERSON BL, GEORGIOU G: Development of an optimized expression system for the screening of antibody libraries displayed on the Escherichia coli surface. Protein. Eng. (1999) 12(7):613-621.
3. VANANTWERP JJ, WITTRUP KD: Fine affinity discrimination by yeast surface display and flow cytometry. Biotechnol. Prog. (2000) 16(1):31-37. This work shows that antibodies having subnanomolar affinity can be selected through yeast display.
4. ERNST W, GRABHERR R, WEGNER D, BORTH N, GRASSAUER A, KATINGER H: Baculovirus surface display: construction and screening of a eukaryotic cpitope library. Nucleic Acids Res. (1998) 26(7):1718-1723.
5. DE HAARD HJ, VAN NEER N, REURS A et al.: A large non-immunized human Fab fragment phage library that permits rapid isolation and kinetic analysis of high affinity antibodies. J. Biol. Chem. (1999) 274(26):18218-18230. This work shows that high-affinity antibodies for essentially any target can be selected from large libraries obtained from naive donors.
6. LADNER RC: Constrained peptides as binding entities. Trends Biotechnol. (1995) 13(10):426-80.
7. HORN TR, WITTINGHOFER A, DE BRUINE AP, HOOGENBOOM HR: Selection of phage-displayed fab antibodies on the active conformation of ras yields a high affinity conformation-specific antibody preventing the binding of c-Raf kinase to Ras. FEBS Lett. (1999) 463(1-2):115-120. This paper shows that antibodies that identify particular conformational states of proteins can be obtained from 'naive' Fab libraries.
8. KINGSBURY GA, JUNGHANS RP: Screening of phage display immunoglobulin libraries by antiM13 ELISA and whole phage PCR. Nucleic Acids Res. (1995) 23(13):2563-2564.
9. EVANS WE, RELLING MV: Pharmacogenomics: translating functional genomics into rational therapeutics. Science (1999) 286:487-491.
10. LUEKING A, HORN M, EICKHOFF H, BUSSOW K, LEHRACH H, WALTER G: Protein microarrays for gene expression and antibody screening. Anal. Biochem. (1999) 270(1):103-111.