Using the SMART(TM) cDNA system to map the transcription initiation site

BioTechniques

Volumn 28, Issue 5, 2000, Pages 846-852

  Gong, Baiwei ^a   Ge, Ruowen ^a  

^a NATIONAL UNIVERSITY OF SINGAPORE   (Singapore)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; DNA SYNTHESIS; GENE MAPPING; NONHUMAN; TRANSCRIPTION INITIATION; TRANSCRIPTION TERMINATION; ANIMAL; CHROMOSOME MAP; DNA SEQUENCE; GENE EXPRESSION REGULATION; GENETIC TRANSCRIPTION; GENETICS; METABOLISM; METHODOLOGY; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; OLIGONUCLEOTIDE PROBE; POLYMERASE CHAIN REACTION; REPORTER GENE; ZEBRA FISH;

COMPLEMENTARY DNA; DYES, REAGENTS, INDICATORS, MARKERS AND BUFFERS; ENDOTHELIAL CELL GROWTH FACTOR; GREEN FLUORESCENT PROTEIN; LYMPHOKINE; PHOTOPROTEIN; VASCULOTROPIN; VASCULOTROPIN A;

ANIMALS; BASE SEQUENCE; CHROMOSOME MAPPING; DNA, COMPLEMENTARY; ENDOTHELIAL GROWTH FACTORS; GENE EXPRESSION REGULATION; GENES, REPORTER; GREEN FLUORESCENT PROTEINS; INDICATORS AND REAGENTS; LUMINESCENT PROTEINS; LYMPHOKINES; MOLECULAR SEQUENCE DATA; OLIGONUCLEOTIDE PROBES; POLYMERASE CHAIN REACTION; SEQUENCE ANALYSIS, DNA; TRANSCRIPTION, GENETIC; VASCULAR ENDOTHELIAL GROWTH FACTOR A; VASCULAR ENDOTHELIAL GROWTH FACTORS; ZEBRAFISH;

EID: 0034185655     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/00285bm04     Document Type: Article

References (4)

1. Chenchik, A., L. Diachenko, F. Moqadam, V. Tarabykin, S. Lukyanov and P.D. Siebert. 1996. Full-length cDNA cloning and determination of mRNA 5′ and 3′ ends by amplification of adaptor-ligated cDNA. BioTechniques 27:526-534.
2. Chenchik, A, Y.Y. Zhu, L. Diatchenko, R. Li, J. Hill and P.D. Siebert. 1998. Generation and use of high-quality cDNA from small amounts of total RNA by SMART PCR, p. 305-319. In P. Siebert and J. Larrick (Eds.), Gene Cloning and Analysis by RT-PCR. Eaton Publishing, Natick, MA.
3. Matz, M, D. Shagin, E. Bogdanova, S. Lukyanov, L. Diatchenko and A. Chenchik. 1999. Amplification of cDNA ends based on template-switching effect and step-out PCR. Nucleic Acids Res. 20:5173-5179.
4. Schwabe, W, J.E. Lee, M. Nathan, R.H. Xu, K. Sitaraman, M. Smith, R.J. Potter, K. Rosenthal et al. 1998. THERMOSCRIPT™ RT, a new avian reverse transcriptase for high-temperature cDNA synthesis to improve RT-PCR. Focus 20:30-33.