Identification of γ-aminobutyric acid transporter (GAT1) on the rat sperm

Cell Research

Volumn 10, Issue 1, 2000, Pages 51-58

  Hu, Jing Hua ^a   He, Xiao Bing ^a   Yan, Yuan Chang ^a  

^a INSTITUTE OF BIOCHEMISTRY AND CELL BIOLOGY   (China)

Author keywords

Expression; Gat1; Rat testis; Sperm

Indexed keywords

MAMMALIA;

4 AMINOBUTYRIC ACID; 4 AMINOBUTYRIC ACID CARRIER; CARRIER PROTEIN; GABA PERMEASE; MEMBRANE PROTEIN; MESSENGER RNA; ORGANIC ANION TRANSPORTER; SLC6A1 PROTEIN, RAT;

ANIMAL; ARTICLE; GENETICS; IMMUNOHISTOCHEMISTRY; MALE; METABOLISM; RAT; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SPERMATOZOON; TESTIS;

ANIMALS; CARRIER PROTEINS; GABA PLASMA MEMBRANE TRANSPORT PROTEINS; GAMMA-AMINOBUTYRIC ACID; IMMUNOHISTOCHEMISTRY; MALE; MEMBRANE PROTEINS; MEMBRANE TRANSPORT PROTEINS; ORGANIC ANION TRANSPORTERS; RATS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, MESSENGER; SPERMATOZOA; TESTIS;

EID: 0034151624     PISSN: 10010602     EISSN: None     Source Type: Journal    
DOI: 10.1038/sj.cr.7290035     Document Type: Article

References (21)

1. Krnjevic K. Chemical nature of synaptic transmission in vertebrates. Physiol Rev 1974; 54:418-40.
2. Roberts E. GABA: the road to neurotransmitter status, in Benzodiazepine/GABA Receptors and Chloride Channels. In: Structure and Functional Properties (Olsen R. W. and Venter J.C. eds) Alan R Liss New York 1986:1-39.
3. Erdö S Wollf JR. γ-Aminobutyric acid outside the mammalian brain. J Neurochem 1990; 54:363-72.
4. Orensanz LM, Fernandez I, Martin del Rio R, Storm-Mathisen J. Gamma-aminobutyric acid in the rat oviduct. Adv Biochem Psychopharmacol 1986; 42:265-74.
5. Bedford JM. Sperm capacitation and fertilization in mammals. Biol Reprod 1970; 2(suppl):128-58.
6. Erdö SL, Welerle L. GABAA-type binding sites on membranes of spermatozoa. Life Sci 1990; 47:1147-51.
7. Roldan RS. Murase T, Shi QX. Exocytosis in spermatozoa in response to progesterone and zona pellucida. Science 1994; 266:1578-81.
8. Aanesen A, Fried G, Andersson E, Gottleb C. Carrier-mediated Gamma-aminobutyricaciduptake inhuman spermatozoa indicating the presence of a high-affinity Gamma-aminobutyric acid transporter protein. Biol Reprod 1996; 54:841-6.
9. Aanesen A, Fried G, Andersson E, Gottleb C. Progesterone stimulates GABA uptake in human spermatozoa. Biochem Biophys Res Commun 1996; 226:88-93.
10. Janet A Clark, Susan G Amara. Amino acid neurotransmitter transporters: structure, function, and molecular diversity. Bioessays 1993; 15(5):323-32.
11. Liu QR, Lopez Corvuera B, Mandiyan S, Nelson H, Nelson N. Molecular characterization of four pharmacologically distinct γ-aminobutyric acid in mouse brain. J Biochem 1993; 208:2106-12.
12. Andah GA, Buckland RB. The uptake of glycerol, γ-aminoisobutyric acid and 2-deoxy-D-glucose by spermatozoa of a line of chickens selected for fertility of frozen-thrawed semen and a control line. Theriogenology 1982; 17:401-8.
13. Boldizsar HK, Wekerle L, Vv́n E, Sarlés P, Barna J. Neurotransmitter amino acids as modulators of biological processes of spermatozoa. In: Erdö SL (ed.), GABA Outside the CNS. Berlin: Springer Verlag; 1992:199-211.
14. Parrish JJ, Foote RH. Quantification of bovine sperm separation by a swim-up method. Relationship to sperm motility, integrity of acrosomes, sperm migration in polyacrylamide gel and fertility. J Androl 1987 Jul-Aug; 8(4):259-66.
15. Laemmli UK. Cleavage of structural proteins during the assembly of the head ofbactriophage T4. Nature 1970; 227:680.
16. Shi QX, Roldan ER. Evidence that a GABAA-like receptor is involved in progesterone-induced acrosomal exocytosis in mouse spermatozoa. Biol Reprod 1995 Feb 52:2373-81.
17. Guastella J, Nelson N, Nelson H, CzyzykL, Keynan S, Miekel MC, Davidson N, Lester HA, Kanner BI. Cloning and expression of rat brain GABA transporter. Science, 1990; 249:1303-6.
18. Tillakaratne NJ, Medina Kauwe L, Gibson KM. Gamma-aminobutyric acid (GABA) metabolism in mammalian neural and nonneural tissues. Comp Biochem Physiol A Physiol 1995 Oct; 112(2):247-63.
19. Mager S, Naeve J, Quick M, Labarca C, Davidson N, Letter HA. Steady states, charge movements, and rates for a cloned GABA transporter expressed in Xenopus oocytes. Neuron 1993; 10:177-88.
20. Kanner BI, Bendahan A, Radian R. Efflux and exchange of γ-aminobutyric acid and nipecotic acid catalysed by synaptic plasma membrane vesicles isolated from immature rat brain. Biochem Biophys Acta 1983; 731:54-62.
21. Harvey M Florman, Christophe Arnoult, Imrana G Kazam, Chongqing Li, Christine MB O'Toole. A Perspective on the control of mammalian fertilization by egg-activated ion channels in sperm: a tale of two channels. Biol Reprod 1998; 59:12-16.