Determination of protein for studies of marine herbivory: A comparison of methods

Journal of Experimental Marine Biology and Ecology

Volumn 244, Issue 1, 2000, Pages 45-65

  Crossman, D J ^a   Clements, K D ^a   Cooper, G J S ^a  

^a UNIVERSITY OF AUCKLAND   (New Zealand)

Author keywords

Algae; Amino acid analysis; Herbivorous fish; Interference; Nitrogen; Protein assays

Indexed keywords

AMINO ACID ANALYSIS; ANALYTIC METHOD; ARTICLE; EXTRACTION; INTERMETHOD COMPARISON; MARINE ENVIRONMENT; NONHUMAN; PHOTOMETRY; PROTEIN CONTENT; PROTEIN DETERMINATION; SAMPLING; SPECTROPHOTOMETRY; TECHNIQUE;

KYPHOSUS SYDNEYANUS;

EID: 0034142320     PISSN: 00220981     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0022-0981(99)00126-4     Document Type: Article

References (58)

1. Berges J.A., Fisher A.E., Harrison P.J. A comparison of Lowry, Bradford and Smith protein assays using different standards and protein isolated from the marine diatom Thalassiosira pseudonana. Mar. Biol. 115:1993;187-193.
2. Bergmeyer H.U., Bernt E., Schmidt F., Stork H. D-Glucose determination with hexokinase and glucose-phosphate dehydrogenase. Bergmeyer H.U. Methods of Enzymatic Analysis. 1974;1196-1201 Academic Press, New York.
3. Bowen S.H. Detrital nonprotein amino acids are the key to rapid growth of Tilapia in Lake Valencia, Venezuela. Science. 207:1980;1216-1218.
4. Bradford M.M. A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:1976;248-254.
5. Brown R.E., Jarvis K.L., Hyland K.J. Protein measurement using bicinchoninic acid: elimination of interfering substances. Anal. Biochem. 180:1989;136-139.
6. Chapman A.R.O., Craigie J.S. Seasonal growth in Laminaria longicruris: Relations with dissolved inorganic nutrients and internal reserves of nitrogen. Mar. Biol. 40:1977;197-205.
7. Choat J.H. The biology of herbivorus fishes on coral reefs. Sale P.F. The Ecology of Fishes On Coral Reefs. 1991;Academic Press, San Diego.
8. Choat J.H., Clements K.D. Vertebrate herbivores in marine and terrestrial environments: A nutritional ecology perspective. Annu. Rev. Ecol. Syst. 29:1998;375-403.
9. Chu F.E., Casey B.B. A comparison of protein assays for oyster larval proteins using two different standards. Mar. Chem. 19:1986;1-7.
10. Clayton J.R., Dortch Q., Thoresen S.S., Ahmed S.I. Evaluation of methods for the separation of proteins and free amino acids in phytoplankton samples. J. Plankton Res. 10:1988;341-358.
11. Clements K.D. Fermentation and gastrointestinal microorganisms in fishes. Mackie R.I., White B.A. Ecology and Physiology of Gastrointestinal Microbes. 1997;156-198 Chapman and Hall, London.
12. Clements K.D., Choat J.H. Comparison of herbivory in the closely-related marine fish genera Girella and Kyphosus. Mar. Biol. 127:1997;579-586.
13. Cowie G.L., Hedges J.I. Sources and reactivities of amino acids in a coastal marine environment. Limnol. Oceanogr. 37:1992;703-724.
14. Cowie G.L., Hedges J.I. Improved amino acid quantification in environmental samples: charge-matched recovery standards and reduced analysis time. Mar. Chem. 37:1992;223-238.
15. Davis E.M. Protein assays: a review of common techniques. Am. Biotechnol. Lab. 6:1988;28-37.
16. Fleurence J., Coeur C.L., Mabeau S., Maurice M., Landrein A. Comparison of different extractive procedures for proteins from the edible seaweeds Ulva rigida and Ulva rotundata. J. Appl. Phycol. 7:1995;577-582.
17. Folch J., Lees M., Sloane G.Y. A simple method for the isolation and purification of total lipids from animal tissues. J. Biol. Chem. 226:1957;497-507.
18. Fris M.B., Horn M.H. Effects of diets of different protein content on food consumption, gut retention, protein, and growth of Cebidichthys violaceus (Girard), an herbivorous fish of temperate zone marine waters. J. Exp. Mar. Biol. Ecol. 166:1993;185-202.
19. Galetto M.J., Bellwood D.R. Digestion of algae by Stegases nigricans and Amphiprion akindynos (Pisces: Pomacentidae), with an evaluation of methods used in digestibility studies. J. Fish Biol. 44:1994;415-428.
20. Gnaiger E., Bitterlich G. Proximate biochemical composition and caloric content calculated from elemental CHN analysis: a stoichiometric concept. Oecologia. 62:1984;289-298.
21. Gomez I., Westermeier R. Energy contents and organic constituents in Antarctic and south Chilean brown algae. Polar Biol. 15:1995;597-602.
22. Harrison P.J., Thomas T.E. Biomass measurements: protein determination. Lobban C.S., Chapman D.J., Kremer B.P. Experimental Phycology. 1988;27-34 Cambridge University Press, Cambridge.
23. Heinrikson R.L., Meredith S.C. Amino acid analysis by reverse-phase high-performance liquid chromatography: precolumn derivatization with phenylisothiocyanate. Anal. Biochem. 136:1984;65-74.
24. Horn M.H. Biology of marine herbivorous fishes. Oceanogr. Mar. Biol. Annu. Rev. 27:1989;167-272.
25. Horn M.H., Mailhiot K.F., Fris M.B., McClanahan L.L. Growth, consumption, assimilation and excretion in the marine herbivorous fish Cebidichthys violaceus (Girard) fed natural and high protein diets. J. Exp. Mar. Biol. Ecol. 190:1995;97-108.
26. Izhaki I. Influence of nonprotein nitrogen on estimation of protein from total nitrogen in fleshy fruits. J. Chem. Ecol. 19:1993;2605-2615.
27. Kaehler S., Kennish R. Summer and winter comparison in the nutritional value of marine macroalgae from Hong Kong. Bot. Mar. 39:1996;11-17.
28. Kessler R.J., Fanestil D.D. Interference by lipids in the determination of protein using bicinchoninic acid. Anal. Biochem. 159:1986;138-142.
29. Kochert G. Protein determination by dye binding. Hellebust J.A., Craigie J.S. Handbook of Phycological Methods: Physiological and Biochemical Methods. 1978;91-93 Cambridge University Press, Cambridge.
30. Lawrence J.M., McClintock J.B. Allocation of organic material and energy to the holdfast, stipe, and fronds in Postelsia palmaeformis (Phaeophyta: Laminariales) on the California coast. Mar. Biol. 99:1988;151-155.
31. Lowry O.H., Rosebrough N.J., Farr A., Randell R. Protein measurement with Folin-phenol reagent. J. Biol. Chem. 193:1951;265-275.
32. Matto R.l., Ishaq M., Saleemuddin M. Protein assay by coomassie brilliant blue G-250-binding method is unsuitable for plant tissues rich in phenols and phenolases. Anal. Biochem. 163:1987;376-384.
33. Mayer L.M., Schick L.L., Sawyer T., Plante C.J., Jumars P.A., Self R.L. Bioavailable amino acids in sediments: A biomimetic, kinetics-based approach. Limnol. Oceanogr. 40:1995;511-520.
34. Mayer L.M., Schick L.l., Self R.F.L., Jumars P.A., Findlay R.H., Chen Z., Sampson S. Digestive environments of benthic macroinvertebrate guts: Enzymes, surfactants and dissolved organic matter. J. Mar. Res. 55:1997;785-812.
35. Mayer L.M., Schick L.l., Setchell F.W. Measurement of protein in nearsure marine sediments. Mar. Ecol. Prog. Ser. 30:1986;159-165.
36. McCleary B.V., Solah V., Gibson T.S. Quantitative measurement of total starch in cereals, flours and products. J. Cereal Sci. 20:1981;51-58.
37. Montgomery W.L., Gerking S.D. Marine macroalgae as foods for fishes: an evaluation of potential food quality. Environ. Biol. Fishes. 5:1980;143-153.
38. Mouradi-Givernaud A., Givernaud T., Morvan H., Cosson J. Annual variations of the biochemical composition of Gelidium latifolium Thuret et Bornet. Hydrobiologia. 260/261:1993;607-612.
39. Neighbours M.A., Horn M.H. Nutritional quality of macrophytes eaten and not eaten by two temperate-zone herbivorous fishes: a multivariate comparison. Mar. Biol. 108:1991;471-476.
40. Nguyen R.T., Harvey H.N. A rapid micro-scale method for the extraction and analysis of protein in marine samples. Mar. Chem. 45:1994;1-14.
41. Peters W.H.M., Fleuren-Jakobs A.M.M., Kamps K.M.P., Pont J.J.H., Bonting S.L. Lowry protein determination on membrane preparations: Need for standardization by amino acid analysis. Anal. Biochem. 124:1982;349-352.
42. Peterson G.L. A simplification of the protein assay method of Lowry et al. which is generally more applicable. Anal. Biochem. 83:1977;346-356.
43. Peterson G.L. Review of the Folin phenol protein quantification method of Lowry, Rosenbrough, Farr and Randall. Anal. Biochem. 100:1978;201-220.
44. Peterson G.L. Determination of total protein. Methods Enzymol. 91:1983;95-119.
45. Rimmer D.W., Wiebe W.J. Fermentative microbial digestion in herbivorous fishes. J. Fish Biol. 31:1987;229-236.
46. Salo-Väänänen P.P., Koivistoinen P.E. Determination of protein in foods: comparison of net protein and crude protein (Nx6.25) values. Food Chem. 57:1996;27-31.
47. Setchell F.W. Particulate protein measurement on oceanographic samples by dye binding. Mar. Chem. 10:1981;301-313.
48. Smith P.L., Krohn R.I., Hermanson G.T., Mallia A.K., Gartner F.H., Provenzano M.D., Fujimoto E.K., Goeke N.M., Olson B.J., Klenk D.C. Measurement of protein using bicinchoninic acid. Anal. Biochem. 150:1985;76-85.
49. Stevens C.E., Hume I.D. Comparative Physiology of the Vertebrate Digestive System. 2nd ed :1995;Cambridge University Press, Cambridge.
50. Sturm E.A., Horn M.H. Food habits, gut morphology and pH, and assimilation efficiency of the Zebra Perch Hermosilla azurea, an herbivorous kyphosid fish of temperate marine waters. Mar. Biol. 132:1998;512-522.
51. Targett N.M., Arnold T. Predicting the effects of brown algal phlorotannins on marine herbivores in tropical and temperate oceans. J. Phycol. 34:1998;195-205.
52. Vial, T.H., 1997. The comparative feeding biology of two temperate water herbivorous fish, silver drummer, Kyphousus sydneyanus,and parore, Girella tricuspidata. Masters Thesis. University of Auckland.
53. Westermeier R., Gomez I. Biomass, energy contents and major organic compounds in the brown alga Lessonia nigrescens (Laminariales Phaeophyceae) from Mehum, South Chile. Bot. Mar. 39:1996;553-559.
54. White T.C.R. The Inadequate Environment: Nitrogen and the Abundance of Animals. 1993;Springer, Berlin.
55. Wiechelman K.J., Braun R.D., Fitzpatrick J.D. Investigation of the bicinchoninic acid protein assay: identification of the groups responsible for color formation. Anal. Biochem. 175:1988;231-237.
56. Wilson S., Bellwood D.R. Cryptic dietary components of territorial damselfishes (Pomacentridae Labroidei). Mar. Ecol. Prog. Ser. 153:1997;299-310.
57. Zamer W.E., Shick J.M., Tapley D.W. Protein measurement and energetic considerations: Comparisons of biochemical and stoichiometric methods using bovine serum albumin and protein isolated from sea anemones. Limnol. Oceanogr. 34:1989;256-263.
58. Zemke-White W.L., Clements K.D. Chlorophyte and rhodophyte starches as factors in diet choice by marine herbivorous fish. J. Exp. Mar. Biol. Ecol. 240:1999;137-149.