Electrophysiological properties of microglial cells in normal and pathologic rat brain slices

European Journal of Neuroscience

Volumn 12, Issue 6, 2000, Pages 2049-2058

  Boucsein, Clemens ^a   Kettenmann, Helmut ^a   Nolte, Christiane ^a  

^a MAX DELBRÜCK CENTER FOR MOLECULAR MEDICINE   (Germany)

Author keywords

Activation; Facial nerve axotomy; Patch clamp; Potassium channels; Resting microglia

Indexed keywords

LIPOPOLYSACCHARIDE; POTASSIUM; POTASSIUM CHANNEL;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; BRAIN CORTEX; BRAIN ELECTROPHYSIOLOGY; CELL ACTIVATION; CORPUS STRIATUM; FACIAL NERVE; FACIAL NERVE NUCLEUS; FEMALE; MEMBRANE CURRENT; MICROGLIA; NERVE CELL MEMBRANE; NERVE CELL MEMBRANE POTENTIAL; NERVE FIBER TRANSECTION; NONHUMAN; PATCH CLAMP; PRIORITY JOURNAL; RAT; TIME;

ANIMALS; ANIMALS, NEWBORN; AXOTOMY; CELL SIZE; CELLS, CULTURED; CEREBRAL CORTEX; FACIAL NERVE; FEMALE; LECTINS; LIPOPOLYSACCHARIDES; MEMBRANE POTENTIALS; MICROGLIA; PATCH-CLAMP TECHNIQUES; PLANT LECTINS; POTASSIUM CHANNELS; RATS; RATS, WISTAR;

EID: 0034129303     PISSN: 0953816X     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1460-9568.2000.00100.x     Document Type: Article

References (34)

1. Acarin, L., Vela, J.M., Gonzalez, B. & Castellano, B. (1994) Demonstration of poly-N-acetyl lactosamine residues in ameboid and ramified microglial cells in rat brain by tomato lectin binding. J. Histochem. Cytochem., 42, 1033-1041.
2. Amigorena, S., Choquet, D., Teillaud, J.L., Korn, H. & Fridman, W.H. (1990) Ion channel blockers inhibit B cell activation at a precise stage of the GI phase of the cell cycle. Possible involvement of K+ channels. J. Immunol., 144, 2038-2045.
3. Brockhaus, J., Ilschner, S., Banati, R.B. & Kettenmann, H. (1993) Membrane properties of ameboid microglial cells in the corpus callosum slice from early postnatal mice. J. Neurosci., 13, 4412-1421.
4. Draheim, H.J., Prinz, M., Weber, J.R., Weiser, T., Kettenmann, H. & Hanisch, U.K. (1999) Induction of potassium channels in mouse brain microglia: Cells acquire responsiveness to pneumococcal cell wall components during late development. Neuroscience, 89, 1379-1390.
5. Eder, C. (1998) Ion channels in microglia (brain macrophages). Am. J. Physiol, 275, C327-C342.
6. Eder, C., Fischer, H.G., Hadding, U. & Heinemann, U. (1995) Properties of voltage-gated potassium currents of microglia differentiated with granulocyte/macrophage colony-stimulating factor. J. Membr. Biol., 147, 137-146.
7. Eder, C. & Heinemann, U. (1996) Proton modulation of outward K+ currents in interferon-gamma-activated microglia. Neurosci. Lett., 206, 101-104.
8. Eder, C., Klee, R. & Heinemann, U. (1997) Distinct soluble astrocytic factors induce expression of outward K+ currents and ramification of brain macrophages. Neurosci. Lett., 226, 147-150.
9. Fischer, H.G., Eder, C., Hadding, U. & Heinemann, U. (1995) Cytokine-dependent K+ channel profile of microglia at immunologically defined functional states. Neuroscience, 64, 183-191.
10. Gerdes, J., Lemke, H., Baisch, H., Wacker, H.H., Schwab, U. & Stein, H. (1984) Cell cycle analysis of a cell proliferation-associated human nuclear antigen defined by the monoclonal antibody Ki-67. J. Immunol., 133, 1710-1715.
11. Giulian, D. & Baker, T.J. (1986) Characterization of ameboid microglia isolated from developing mammalian brain. J. Neurosci., 6, 2163-2178.
12. Graeber, M.B., Banati, R.B., Streit, W.J. & Kreutzberg, G.W. (1989) Immunophenotypic characterization of rat brain macrophages in culture. Neurosci. Lett., 103, 241-246.
13. Graeber, M.B., Streit, W.J. & Kreutzberg, G.W. (1988) Axotomy of the rat facial nerve leads to increased CR3 complement receptor expression by activated microglial cells. J. Neurosci. Res., 21, 18-24.
14. Haas, S., Brockhaus, J., Verkhratsky, A. & Kettenmann, H. (1996) ATP-induced membrane currents in ameboid microglia acutely isolated from mouse brain slices. Neuroscience, 75, 257-261.
15. Hamill, O.P., Marty, A., Neher, E., Sakmann, B. & Sigworth, F.J. (1981) Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches. Pflugers Arch., 391, 85-100.
16. Illes, P., Nörenberg, W. & Gebicke, H.P. (1996) Molecular mechanisms of microglial activation. B. Voltage-and purinoceptor-operated channels in microglia. Neurochem. Int., 29, 13-24.
17. Kettenmann, H., Banati, R. & Walz, W. (1993) Electrophysiological behavior of microglia. Glia, 7, 93-101.
18. Kettenmann, H., Hoppe, D., Gottmann, K., Banati, R. & Kreutzberg, G. (1990) Cultured microglial cells have a distinct pattern of membrane channels different from peritoneal macrophages. J. Neurosci. Res., 26, 278-287.
19. Kloss, C.U., Kreutzberg, G.W. & Raivich, G. (1997) Proliferation of ramified microglia on an astrocyte monolayer: characterization of stimulatory and inhibitory cytokines. J. Neurosci. Res., 49, 248-254.
20. Kreutzberg, G.W. (1996) Microglia: a sensor for pathological events in the CNS. Trends Neurosci., 19, 312-318.
21. Lawson, L.J., Matyszak, M.K. & Perry, V.H. (1993) Lessons from microglia in special sites. Clin. Neuropathol., 12, 310-313.
22. Lawson, L.J., Perry, V.H., Dri, P. & Gordon, S. (1990) Heterogeneity in the distribution and morphology of microglia in the normal adult mouse brain. Neuroscience, 39, 151-170.
23. Lepple-Wienhues, A., Berweck, S., Bohmig, M., Leo, C.P., Meyling, B., Garbe, C. & Wiederholt, M. (1996) K+ channels and the intracellular calcium signal in human melanoma cell proliferation. J. Membr. Biol., 151, 149-157.
24. Lewis, R.S. & Cahalan, M.D. (1995) Potassium and calcium channels in lymphocytes. Annu. Rev. Immunol., 13, 623-653.
25. Nörenberg, W., Gebicke, H.P. & Illes, P. (1992) Inflammatory stimuli induce a neu K+ outward current in cultured rat microglia. Neurosci. Lett., 147, 171-174.
26. Nörenberg, W., Gebicke, H.P. & Illes, P. (1994) Voltage-dependent potassium channels in activated rat microglia. J. Physiol. (Lond.), 475, 15-32.
27. Raivich, G., Bohatschek, M., Kloss, C.U., Werner, A., Jones, L.L. & Kreutzberg, G.W. (1999) Neuroglial activation repertoire in the injured brain: graded response, molecular mechanisms and cues to physiological function. Brain Res. Rev., 30, 77-105.
28. Schlichter, L.C., Sakellaropoulos, G., Ballyk, B., Pennefather, P.S. & Phipps, D.J. (1996) Properties of K+ and Cl-channels and their involvement in proliferation of rat microglial cells. Glia, 17, 225-236.
29. Schmidtmayer, J., Jacobsen, C., Miksch, G. & Sievers, J. (1994) Blood monocytes and spleen macrophages differentiate into microglia-like cells on monolayers of astrocytes: membrane currents. Glia, 12, 259-267.
30. Sparapani, M., Buonamici, L., Ciani, E., Battelli, M.G., Ceccarelli, G., Stirpe, F. & Contestabile, A. (1997) Toxicity of ricin and volkensin, two ribosome-inactivating proteins, to microglia, astrocyte, and neuron cultures. Glia, 20, 203-209.
31. Streit, W.J. (1990) An improved staining method for rat microglial cells using the lectin from Griffonia simplicifolia (GSA I-B4). J. Histochem. Cytochem., 38, 1683-1686.
32. Streit, W.J. & Kreutzberg, G.W. (1988) Response of endogenous glial cells to motor neuron degeneration induced by toxic ricin. J. Comp. Neural., 268, 248-263.
33. Visentin, S., Agresti, C., Patrizio, M. & Levi, G. (1995) Ion channels in rat microglia and their different sensitivity to lipopolysaccharide and interferon-gamma. J. Neurosci. Res., 42, 439-451.
34. Zielasek, J. & Hartung, H.P. (1996) Molecular mechanisms of microglial activation. Adv. Neuroimmunol., 6, 191-122.