Novel approach for large-scale, biocompatible, and low-cost fractionation of peptides in proteolytic digest of food protein based on the amphoteric nature of peptides

Journal of Agricultural and Food Chemistry

Volumn 48, Issue 5, 2000, Pages 1955-1959

  Akahoshi, Asuka ^a,b   Sato, Kenji ^a   Nawa, Yuko ^a   Nakamura, Yasushi ^a   Ohtsuki, Kozo ^a  

^a KYOTO PREFECTURAL UNIVERSITY   (Japan)

^b PREFECTURAL UNIVERSITY OF KUMAMOTO   (Japan)

Author keywords

Autofocusing; Electrophoresis; Functional foods; Peptide fractionation; Preparative isoelectric focusing

Indexed keywords

AGAROSE; CASEIN; PEPTIDE; SODIUM HYDROXIDE; TRYPSIN;

ARTICLE; BIOCOMPATIBILITY; CHEMISTRY; COST; ELECTRIC POTENTIAL; ELECTRICITY; FOOD; FRACTIONATION; HYDROLYSIS; ISOELECTRIC FOCUSING; ISOLATION AND PURIFICATION; METHODOLOGY; PH; PROTEIN DEGRADATION;

CASEINS; COSTS AND COST ANALYSIS; FOOD; HYDROLYSIS; ISOELECTRIC FOCUSING; PEPTIDES; TRYPSIN;

EID: 0034127373     PISSN: 00218561     EISSN: None     Source Type: Journal    
DOI: 10.1021/jf991286y     Document Type: Article

References (20)

1. Acevedo, F. Isotachophoresis of proteins in sucrose density gradients. Electrophoresis 1993, 14, 1019-1022.
2. Arai, S. Studies on functional foods in Japan - state of the art. Biosci., Biotechnol., Biochem. 1996, 60, 9-15.
3. Bidlingmeyer, B. A.; Cohen, S. A.; Tarvin, T. L. Rapid analysis of amino acids using pre-column derivatization. J. Chromatogr. 1984, 336, 93-104.
4. Bier, M.; Long, T. Recycling isoelectric focusing: use of simple buffers. J. Chromatogr. 1992, 604, 73-83.
5. Egen, N. B.; Bliss, M.; Mayersohn, M.; Owens, S. M.; Arnold, L.; Bier, M. Isolation of monoclonal antibodies to phencyclidine from ascites fluid by preparative isoelectric focusing in the Rotofor. Anal. Biochem. 1988, 172, 488-494.
6. Habibi-Najafi, M. B.; Lee, B. H. Bitterness in cheese: a review. Crit. Rev. Food Sci. Nutr. 1996, 36, 397-411.
7. Haglund, H. Isoelectric focusing in natural pH gradients - A technique of growing importance for fractionation and characterization of proteins. Sci. Tools 1967, 14, 17-23,
8. Huang, J. X.; Guiochon, G. Applications of preparative high-performance liquid chromatography to the separation and purification of peptides and proteins. J. Chromatogr. 1989, 492, 431-469.
9. Mant, C. T.; Zhou, N. E.; Hodges, R. S. Amino acids and peptides. In Chromatography Part B: Applications; Heftman, E., Ed.; Elsevier Science Publishers: Amsterdam, The Netherlands, 1992; pp 75-150.
10. Meisel, H. Biochemical properties of regulatory peptides derived from milk proteins.Biopolymer 1997, 43, 119-128.
11. Mills, E. N. C.; Alcocer, J. C. M.; Morgan, M. R. A. Biochemical interactions of food-derived peptides. Trends Food Sci. Technol. 1992, 3, 64-68.
12. Minamimura, N.; Matsumura, Y.; Fukumoto, J.; Yamamoto, T. Bitter peptides in cow milk casein digests with bacterial proteinase. Agric. Biol. Chem. 1972, 36, 588-595.
13. Osajima, K.; Sakakibara, S.; Sawabe, T.; Kitamura, K.; Matsuda, H.; Osajima, Y. Study on separation system of angiotensin I converting enzyme inhibitory peptides originating from sardine meat. Nippon Shokuhin Kogyo Gakkaishi 1993, 40, 568-576.
14. Righetti, P. G.; Wenisch, E.; Faupel, M. Preparative protein purification in a multi-compartment electrolyser with immobiline membranes. J. Chromatogr. 1989, 475, 293-309.
15. Rilbe, H. Isoelectric focusing - development from notion to practically working tool. Sci. Tools 1976, 23, 18-22.
16. Roman, M. C.; Brown, P. R. Free-flow electrophoresis as a preparative separation technique. Anal. Chem. 1994, 66, 86-94.
17. Sato, K.; Tsukamasa, Y.; Imai, C.; Ohtsuki, K.; Shimizu, Y.; Kawabata, M. Improved method for identification and determination of ∈-(γ-glutamyl)-lysine cross-link in protein using proteolytic digestion and derivatization with phenyl isothiocyanate followed by high-performance liquid chromatography separation. J. Agric. Food Chem. 1992, 40, 806-810.
18. Sato, K.; Nishimura, R.; Suzuki, Y.; Motoi, H.; Nakamura, Y.; Ohtsuki, K.; Kawabata, M. Occurrence of indigestible pyroglutamyl peptides in an enzymatic hydrolysate of wheat gluten prepared on an industrial scale. J. Agric. Food Chem. 1998, 46, 3403-3405.
19. Stephen, A. M. Regulatory aspects of functional products. In Functional Foods Biochemical and Processing Aspects; Mazza, G., Ed.; Technomic Publishing: Lancaster, PA, 1998; pp 403-437.
20. Yata, M.; Sato, K., Ohtsuki, K.; Kawabata, M. Fractionation of peptides in protease digests of proteins by preparative isoelectric focusing in the absence of added ampholyte: A biocompatible and low-cost approach referred to as autofocusing. J. Agric. Food Chem. 1996, 44, 76-79.