PCR-RFLP based screening of Frankia in alder nodules having different levels of nitrogenase activity

Symbiosis

Volumn 28, Issue 4, 2000, Pages 337-350

  Verghese, Susamma K ^a   Misra, Arvind K ^a  

^a NORTH EASTERN HILL UNIVERSITY   (India)

Author keywords

Alder; Frankia; Nitrogenase activity; PCR RFLP

Indexed keywords

ACETYLENE; ASSAY; ENZYME ACTIVITY; INDIA; MICROORGANISM SCREENING; NITROGEN FIXATION; NITROGENASE; NODULATION; POLYMERASE CHAIN REACTION; RESTRICTION FRAGMENT LENGTH POLYMORPHISM;

ENZYME ACTIVITY; NITROGEN FIXATION; TREE;

INDIA;

ALNUS NEPALENSIS; FRANKIA;

EID: 0034126714     PISSN: 03345114     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (23)

1. Baker, D. and O'Keefe, D. 1984. A modified sucrose fractionation procedure for the isolation of frankiae from actinorhizal root nodules and soil samples. Plant and Soil 78: 23-28.
2. Benson, D.R., Stephens, D.W., Clawson, M.L., and Silvester, W.B. 1996. Amplification of 16S rRNA genes from Frankia strains in root nodules of Ceanothus griseus, Coriaria arborea, Coriaria plumosa, Discaria toumatou and Purshia tridentata. Applied and Environmental Microbiology 62: 2904-2909.
3. Bosco, M., Fernandez, M.P., Simonet, P., Materassi, R., and Normand, P. 1992. Evidence that some Frankia strains are able to cross boundaries between Alnus and Elaeagnus host specificity groups. Applied and Environmental Microbiology 58: 1569-1576.
4. Dawson, J.O. and Sun, Soon-Hwa 1981. The effect of Frankia isolates from Comptonia peregrina and Alnus crispa on the growth of Alnus glutinosa, A. cordata and A. incana clones. Canadian Journal of Forest Research 11: 758-762.
5. Dretzen, G., Bellard, M., Sassone-Corsi, P., and Chambon, P. 1981. A reliable method for the recovery of DNA fragments from agarose and acrylamide gels. Analytical Biochemistry 112: 295-298.
6. Embley, T.M., Smida, J., and Stackebrandt, E. 1988. Reverse transcriptase sequencing of 16S rRNA from Faenia rectivirgula, Pseudomonas thermophila and Saccharopolyspora hirsuta, three wall type IV actinomycetes which lack mycolicic acids. Journal of General Microbiology 134: 961-966.
7. Ganesh, G. 1993. Study of genetic diversity of Frankia alni strains isolated from Alnus nepalensis root nodules found in Meghalaya. Ph.D. Thesis, NEHU, Shillong, India.
8. 2 fixing ability among natural isolates of Azospirillum. Microbial Ecology 36: 193-201.
9. Harry, D.E., Yang, D.C., and Dawson, J.O. 1991. Nucleotide sequence and diversity in the 16S ribosomal RNA from Frankia. Plant and Soil 131: 143-146.
10. Kondorosi, A., Svab, A., Kiss, G.B., and Dixon, R.A. 1977. Ammonium assimilation and nitrogen fixation in R. meliloti. Molecular and General Genetics 151: 221-226.
11. Monz, C.A. and Schwintzer, C.R. 1989. The physiology of spore negative and spore positive nodules of Myrica gale. Plant and Soil 118: 75-87.
12. Nazaret, S., Cournoyer, B., Normand, P., and Simonet, P. 1991. Phylogenetic relationships among Frankia genomic species determined by use of amplified 16S rDNA sequences. Journal of Bacteriology 173: 4072-4078.
13. Normand, P., Simonet, P., and Bardin, R. 1988. Conservation of nif sequences in Frankia. Molecular and General Genetics 213: 238-246.
14. Normand, P., Orso, S., Cournoyer, B., Jeannin, P., Chapelon, C., Dawson, J., Evtusenko, L., and Misra, A.K. 1996. Molecular phylogeny of the genus Frankia and related genera and emendation of the family Frankiaceae. International Journal of Systematic Bacteriology 46: 1-9.
15. 2 fixation in Mediterranean rice fields. Microbial Ecology 34: 39-48.
16. Reddell, P. and Bowen, G.D. 1985. Frankia source affects growth, nodulation and nitrogen fixation in Casuarina species. New Phytologist 100: 115-122.
17. Reddell, P., Rosebrook, P.A., Bowen, G.D., and Gwaze, D. 1988. Growth responses in Casuarina cunninghamiana plantings to inoculation with Frankia. Plant and Soil 108: 79-86.
18. Rouvier, C., Prin, Y., Reddell, P., Normand, P., and Simonet, P. 1996. Genetic diversity among Frankia strains nodulating members of the family Casuarinaceae in Australia revealed by PCR and RFLP analysis with crushed root nodules. Applied and Environmental Microbiology 62: 979-985.
19. Sambrook, J., Fritsch, E.F., and Maniatis, T. 1989. Molecular Cloning: A Laboratory Manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York.
20. Simonet, P., Grosjean, M.C., Misra, A.K., Nazaret, S., Cournoyer, B., and Normand, P. 1991. Frankia genus specific characterization by polymerase chain reaction. Applied and Environmental Microbiology 57: 3278-3286.
21. Sougoufara, B., Maggia, L., Duhoux, E., and Dommergues, Y.R. 1992. Nodulation and nitrogen fixation in nine Casuarina clone-Frankia strain combinations. Acta Oecologica 13: 497-503.
22. Sprent, J.I., Sutherland, J.M., and DE Faria, S.M. 1987. Some aspects of the biology of nitrogen fixing organisms. In: A Century of Nitrogen Fixation Research. F.J. Bergersoen and J.R. Postgate, eds. Royal Society, London, pp. 45-63.
23. Stewart, W.P.D., Fitzgerald, G.P., and Burris, R.H. 1968. Acetylene reduction in nitrogen fixing blue-green algae. Archives of Microbiology 62: 336-348.