Oxidative metabolism and release of myeloperoxidase from polymorphonuclear leukocytes obtained from blood sedimentation in a Ficoll-Hypaque gradient

Cell Biochemistry and Function

Volumn 18, Issue 2, 2000, Pages 127-132

  Rebecchi, Ivanise M M ^a   Ferreira Novo, Neil ^a   Julian, Yara ^a   Campa, Ana ^b  

^a UNIVERSITY OF SÃO PAULO   (Brazil)

^b FEDERAL UNIVERSITY OF SÃO PAULO   (Brazil)

Author keywords

Blood cells; Chemiluminescence; Ficoll Hypaque; Myeloperoxidase; Oxidative metabolism; Polymorphonuclear leukocytes; Spontaneous sedimentation

Indexed keywords

DIATRIZOATE; FICOLL; HYDROGEN PEROXIDE; LUMINOL; MYELOPEROXIDASE; REACTIVE OXYGEN METABOLITE;

AEROBIC METABOLISM; ARTICLE; BLOOD; CELL SEPARATION; CHEMOLUMINESCENCE; CONTROLLED STUDY; DENSITY GRADIENT CENTRIFUGATION; ENZYME RELEASE; HUMAN; HUMAN CELL; NEUTROPHIL; NORMAL HUMAN; PRIORITY JOURNAL;

ACRIDINES; BLOOD SEDIMENTATION; CHEMILUMINESCENT MEASUREMENTS; CHEMISTRY, CLINICAL; CYTOCHALASIN B; DIATRIZOATE; FICOLL; HUMANS; HYDROGEN PEROXIDE; INDICATORS AND REAGENTS; LEUKOCYTES, MONONUCLEAR; LUMINOL; N-FORMYLMETHIONINE LEUCYL-PHENYLALANINE; NEUTROPHILS; PEROXIDASE; REACTIVE OXYGEN SPECIES; REPRODUCIBILITY OF RESULTS; TETRADECANOYLPHORBOL ACETATE; ZYMOSAN;

EID: 0034122840     PISSN: 02636484     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1099-0844(200006)18:2<127::AID-CBF865>3.0.CO;2-V     Document Type: Article

References (21)

1. Hirschhorn R, Weissmann G. Isolation and properties of human leukocytes lysosomes in vitro. Proc Soc Exp Med 1965; 119: 36-39.
2. Böyum A. A one-stage procedure for isolation of granulocytes and lymphocytes from human blood, Paper III. Scand J Clin Lab Invest 1968;: 97P: 51-76.
3. Böyum A. Isolation of mononuclear cells and granulocytes from human blood, Paper IV. Scand J Clin Lab Invest 1968; 97P: 77-84.
4. Böyum A. Isolation of leukocytes from human blood. Further observations, Paper III. Scand J Clin Lab Invest 1968; 97P: 31-50.
5. Bainton DF, Ullyot JL, Farquhar MG. The development of neutrophilic polymorphonuclear leukocytes in human bone marrow. J Exp Med 1971; 134: 907-934.
6. Bainton DF. Morphology of neutrophils, eosinophils and basophils. In Hematology, Williams W, Beutler E, Marshall AL, Barrys SC, Thomas JK (eds). McGraw-Hill: New York, 1995; 753-766.
7. 2+-induced exocytosis in individual human neutrophils: high and low affinity granule populations and submaximal responses. Eur Mol Biol Org J 1989; 17: 1279-1288.
8. Solberg CO, Helium KB. Neutrophil granulocyte function in bacteria infections. Lancet 1972; ii: 727-729.
9. Glasser L, Fiedertein R-L. The effect of various cell separation procedures on assays of neutrophil function. A critical appraisal. Am J Clin Pathol 1990; 93: 662-629.
10. 2. Free Rad Biol Med 1993; 15: 447-451.
11. Brestel EP. Co-oxidation of luminol by hypochlorite and hydrogen peroxide - implications for neutrophil chemiluminescence. Biochem Biophys Res Commun 1985; 126: 482-488.
12. Dahlgren C, Stendahl P. Role of myeloperoxidase in luminol-dependent chemiluminescence of polymorphonuclear leukocytes. Infect Immun 1983; 39: 736-741.
13. Stossel TP. Phagocytosis. New Eng J Med 1974; 290: 774-780.
14. 2 production and degranulation. J Leuk Biol 1991; 49: 236-244.
15. Nowak D. Hydrogen peroxide release from human polymorphonuclear leukocytes measured with horseradish peroxidase and o-dianisidine. Effect of various stimulators and cytochalasin B. Biomed Biochim Acta 1990; 49: 353-362.
16. Elferink JGR, Koster BMD, Boonen GJJC. Cytochalasin B-induced superoxide production in polycation-treated neutrophils. Inflammation 1991; 15: 413-425.
17. Dahlgren C. Effects on intra- and extracellular localised, chemo-attractant-induced, oxygen radical production in neutrophils following modulation of conditions for ligand-receptor interaction. Inflammation 1988; 12: 335-349.
18. Tauber AI. Protein kinase C and the activation of the human neutrophil NADPH- oxidase. Blood 1987; 69: 711-720.
19. Grisham MB, Egerson TD, McCord JM, Jones HP. A comparative study of neutrophil purification and function. J Immunol Methods 1985; 82: 315-320.
20. Pember SO, Kinkade JM. Differences in myeloperoxidase activity from neutrophilic polymorphonuclear leukocytes of differing density: relationship to selective exocytosis of distinct forms of the enzyme. Blood 1983; 61: 1116-1124.
21. Miyasaki KT, Song JP, Munthy ARK. Secretion of myeloperoxidase isoforms by human neutrophils. Anal Biochem 1991; 193: 38-44.