Coexpression of cyt1Aa of Bacillus thuringiensis subsp. israelensis with Bacillus sphaericus binary toxin gene in acrystalliferous strain of B. thuringiensis

Current Microbiology

Volumn 40, Issue 5, 2000, Pages 322-326

  Li, Tianyong ^a   Sun, Fan ^a   Yuan, Zhiming ^a,b   Zhang, Yongmei ^a   Yu, Jianxiu ^b   Pang, Yi ^b  

^a WUHAN INSTITUTE OF VIROLOGY   (China)

^b SUN YAT SEN UNIVERSITY   (China)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL TOXIN;

ARTICLE; BACILLUS SPHAERICUS; BACILLUS THURINGIENSIS; BACTERIAL GENE; BACTERIUM TRANSFORMATION; CULEX PIPIENS; CULEX QUINQUEFASCIATUS; GENE EXPRESSION; IMMUNOBLOTTING; LARVA; MOSQUITO; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN INDUCTION; SHUTTLE VECTOR; SPOROGENESIS; TOXICITY;

BACILLUS SPHAERICUS; BACILLUS THURINGIENSIS; BACILLUS THURINGIENSIS SEROVAR ISRAELENSIS; BACTERIA (MICROORGANISMS); CULEX PIPIENS; CULEX PIPIENS QUINQUEFASCIATUS; POSIBACTERIA; PROKARYOTA;

EID: 0034122753     PISSN: 03438651     EISSN: None     Source Type: Journal    
DOI: 10.1007/s002849910064     Document Type: Article

References (19)

1. Bar E, Sandler N, Makayoto M, Keynan A (1998) Expression of chromosomally inserted Bacillus thuringiensis israelensis toxin gene in Bacillus sphaericus. J InvertebrPathol 72:206-213
2. Bourgouin C, Delecluse A, de la Terre F (1990) Transfer of the toxin protein genes of Bacillus sphaericus into Bacillus thuringiensis subsp. israelensis and their expression. Appl Environ Microbiol 56:340-344
3. Brian HB, Jack TT (1989) Transformation of Bacillus cereus vegetative cells by electroporation. Appl Environ Microbiol 55: 1649-1652
4. Broadwell AH, Baumann L, Baumann P (1990) Larvicidal properties of the 42-and 51-kilodalton Bacillus sphaericus proteins expressed in different bacterial hosts: evidence for a binary toxin. Curr Microbiol 21:361-366
5. Charles JF, Nielsen-LeRoux C (1996) Les bacteries entomopathogenes: mode d'action sur les larves de moustiques et phenomenes de resistance. Ann Inst Pasteur (Paris) 7:233-245
6. Crickmore N, Zeigler DR, Feitelson J, Schnepf E, Van Rie J, Lereclus 'D, Baum J, Dean HD (1998) Revision of the nomenclature for the Bacillus thuringiensis pesticidal crystal proteins. Microbiol Mol Biol Rev 62:807-813
7. Delecluse A, Poncet S, Klier A, Rapoport G (1993) Expression of cryIVA and cryIVB genes independently or in combination in a crystal minus strain of Bacillus thuringiensis subsp. israelensis. Appl Environ Microbiol 59:3922-3927
8. Federici BA, Bauer LS (1998) Cyt1Aa protein of Bacillus thuringiensis is toxic to the cottonwood leaf beetle, Chrysomela scripta, and suppresses high levels of resistance to Cry3Aa. Appl Environ Microbiol 64:4368-4371
9. Lacey LA, Singer S (1982) Larvicidal activity of new isolates of Bacillus sphaericus and Bacillus thuringiensis (H14) against anopheline and culicine mosquitoes. Mosq News 42:537-543
10. Maniatis T, Fritsch EF, Sambrook J (1989) Molecular cloning; a laboratory manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press
11. Poncet S, Delecluse A, Klier A, Rapoport G (1995) Evaluation of synergistic interactions among the CryIVA, CryIVB, and CryIVD toxic components of B. thuringiensis subsp. israelensis crystals. J Invertebr Pathol 66:131-135
12. Poncet S, Bernard C. Dervyn E, Cayley J, Klier A, Rapoport G (1997) Improvement of Bacillus sphaericus toxicity against dipteran larvae by integration, via homologous recombination, of the Cry11A toxin gene from Bacillus thuringiensis subsp. israelensis. Appl Environ Microbiol 63:4413-4420
13. Thiery S, Hamon A, Delecluse A, Orduz S (1998) The introduction into Bacillus sphaericus of the Bacillus thuringiensis subsp. medellin cyt1Abl gene results in higher susceptibility of resistant mosquito larva populations to B. sphaericus. Appl Environ Microbiol 64:3910-3916
14. World Health Organization (1985) Informal consultation on the development of Bacillus sphaericus as microbial larvicide. TDR/BCV/SPHAERICUS/85.3. 1-24
15. Writh MC, Georghiou GP, Federici B (1997) CytA enable endotoxins of Bacillus thuringiensis to overcome high levels of Cry IVA resistance in mosquito, Culex quinquefasciatus. Proc Natl Acad Sci USA 94:10536-10540
16. Wu D, Federici BA (1993) A 20-kilodalton protein preserves cell viability and promotes CytA crystal formation during sporulation in Bacillus thuringiensis. J Bacteriol 175:5276-5280
17. Wu D, Johnson J, Federici BA (1994) Synergism of mosquitocidal toxicity between CytA and CryIVD proteins using inclusions produced from cloned genes of Bacillus thuringiensis. Mol Microbiol 13:965-972
18. Yuan ZM, Nielsen-LeRoux C, Pasteur N, Frutos R (1999a) Cloning and expression of the binary toxin genes of Bacillus sphaericus C3-41 in a crystal minus B. thuringiensis subsp. israelensis. Acta Microbiol Sin 39:35-41
19. Yuan ZM, Zhang YM, Cai QX, Liu EY (1999b) High-level field resistance to Bacillus sphaericus C3-41 in Culex quinquefasciatus from Southern China. (Submitted)