Immuno-capture differential display method (IDDM) for the detection of environmentally induced promoters in rhizobacteria

Journal of Microbiological Methods

Volumn 41, Issue 1, 2000, Pages 77-84

  Timms Wilson, T M ^a   Ellis, R J ^a,b   Bailey, M J ^a  

^a CARDIFF UNIVERSITY   (United Kingdom)

^b CARDIFF UNIVERSITY   (United Kingdom)

Author keywords

Lacy antibody capture; lacZY; Mini Tn5; Promoters; Pseudomonads

Indexed keywords

ANTIBODY; ANTISERUM;

ARTICLE; BACTERIUM DETECTION; GENE EXPRESSION; IMMUNOASSAY; MUTAGENESIS; NONHUMAN; OOMYCETES; PRIORITY JOURNAL; PROMOTER REGION; PSEUDOMONADACEAE; PSEUDOMONAS FLUORESCENS; TECHNIQUE; TRANSPOSON;

PROKARYOTA; PSEUDOMONAS; PSEUDOMONAS FLUORESCENS; RHIZOBIALES;

EID: 0034119393     PISSN: 01677012     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0167-7012(00)00139-1     Document Type: Article

References (23)

1. Bailey, M.J., Lilley, A.K., Thompson, I.P., Rainey, P.B., Ellis, R.J., 1995. Site directed chromosomal marking of a fluorescent pseudomonad isolated from the phytosphere of sugar beet; stability and potential for marker gene transfer. Mol. Ecol. 4, 755-763.
2. Barry, G.F., 1988. A broad host range shuttle system for gene insertion into the chromosome of Gram-negative bacteria. Gene 71, 75-84.
3. Buchel, D.E., Gronenborn, B., 1980. Sequence of the lactose permease gene. Nucleic Acids Res. 283, 541-545.
4. Cebolla, A., Guzman, C., de Lorenzo, V., 1996. Non-disruptive detection of activity of catabolic promoters of Pseudomonas putida with an antigenic surface reporter system. Appl. Environ. Microbiol. 62, 214-220.
5. de Lorenzo, V., Herrero, M., Jakubzik, U., Timmis, K.N., 1990. Mini-Tn5 transposon derivatives for insertion mutagenesis, promoter probing, and chromosomal insertion of cloned DNA in Gram-negative eubacteria. J. Bacteriol. 172, 6568-6572.
6. Ellis, R.J., Timms-Wilson, T.M., Beringer, J.M., Rhodes, D.J., Renwick, A., Stevenson, L., Bailey, M.J., 1999. Ecological basis for biocontrol of damping-off disease by Pseudomonas fluorescens 54/96. J. Appl. Microbiol. 87, 454-463.
7. Fedi, S., Brazil, D., Dowling, D., O'Gara, F., 1996. Construction of a modified mini-Tn5lacZY non-antibiotic marker cassette: ecological evaluation of a lacZY marker Pseudomonas strain in the sugar beet rhizosphere. FEMS Microbiol. Lett. 135, 251-257.
8. Fedi, S., Tola, E., Moenne-Loccoz, Y., Dowling, D.N., Smith, L.M., O'Gara, F., 1997. Evidence for signalling between phytopathogenic fungi Pythium ultimum and Pseudomonas fluorescens F113: P. ultimum represses the expression of genes in P. fluorescens F113 resulting in altered ecological fitness. Appl. Environ. Microbiol. 63, 4261-4266.
9. Herrero, M., de Lorenzo, V., Timmis, K.N., 1990. Transposon vectors containing non-antibiotic resistance selection markers for cloning and stable chromosomal insertion of foreign genes in Gram-negative bacteria. J. Bacteriol. 172, 6557-6567.
10. Mahan, M.J., Slauch, J.M., Mekalanos, J.J., 1993. Selection of bacterial virulence genes specifically induced in host tissue. Science 259, 686-688.
11. Morgan, J.A.W., Winstanley, C., Pickup, R.W., Jones, J.G., Saunders, J.R., 1989. Direct phenotypic and genotypic detection of a recombinant pseudomonad population released into lake water. Appl. Environ. Microbiol. 55, 2537-7544.
12. Osbourn, A.E., Barber, C.E., Daniels, M.J., 1987. Identification of plant induced genes of the bacterial pathogen Xanthomonas campestris pathovar campestris using a promoter probe plasmid. EMBO J. 6, 23-328.
13. Prosser, J.I., 1994. Molecular marker systems for detection of genetically engineered microorganisms in the environment. Microbiology 140, 5-17.
14. Qoronfleh, M.W., Debouck, C., Keller, J., 1992. Identification and characterisation of novel low temperature inducible promoters of Escherichia coli. J. Bacteriol. 174, 7902-7909.
15. Rainey, P.B., 1999. Adaptation of Pseudomonas fluorescens genes to the plant rhizosphere. Environ. Microbiol. 1, 243-258.
16. Rainey, P.B., Bailey, M.J., 1996. Physical and genetic map of the Pseudomonas fluorescens SBW25 chromosome. Mol. Microbiol. 19, 521-533.
17. Rainey, P.B., Bailey, M.J., Thompson, I.P., 1994. Phenotypic and genotypic diversity of fluorescent pseudomonads isolated from field grown sugar beet. Microbiology 140, 2315-2331.
18. Sambrook, J., Fritsch, E.F., Maniatis, T., 1989. In: Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
19. Smith, D.B., Johnson, K.S., 1988. Single step purification of polypeptides in Escherichia coli as fusions with glutathione S-transferase. FEMS Microbiol. Lett. 67, 31-40.
20. Thompson, I.P., Lilley, A.K., Ellis, R.J., Bramwell, P.A., Bailey, M.J., 1995. Survival, colonisation and dispersal of genetically modified Pseudomonas fluorescens SBW25 in the phytosphere of field grown sugar beet. Nature/BioTechniques 13, 1493-1497.
21. Timms-Wilson, T.M. 1998. Molecular study and genetic approaches for improving the biological control activity of Pseudomonas fluorescens 54/96 in the suppression of Pythium ultimum in seedlings (Damping-off disease). D.Phil Thesis, University of Oxford, pp. 171.
22. Vieira, J., Messing, J., 1982. The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. FEMS Microbiol. Lett. 19, 259-268.
23. Wang, J., Mushegian, A., Lory, S., Jin, S., 1996. Large scale isolation of candidate virulence genes of Pseudomonas aeruginosa by in vivo selection. Proc. Natl Acad Sci USA 93, 10434-10439.