Extraction of nuclear proteins with increased DNA binding activity

BioTechniques

Volumn 28, Issue 5, 2000, Pages 938-942

  Slomiany, Beatrix A ^a   Kelly, Margaret M ^a   Kurtz, David T ^a,b  

^a MEDICAL UNIVERSITY OF SOUTH CAROLINA   (United States)

^b MEDICAL UNIVERSITY OF SOUTH CAROLINA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANIMAL CELL; ARTICLE; MOUSE; NONHUMAN; NUCLEOTIDE SEQUENCE; PROTEIN DNA BINDING; PROTEIN DNA INTERACTION; PROTEIN ISOLATION; TRANSCRIPTION REGULATION;

EID: 0034118386     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/00285st08     Document Type: Article

References (6)

1. Andrews, N.C. and D.V. Faller. 1991. A rapid micropreparation technique for extraction of DNA binding proteins from limiting numbers of mammalian cells. Nucleic Acids Res. 19:2499.
2. Dignam, J., R.M. Lebovitz and R.R. Roeder. 1983. Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei. Nucleic Acids Res. 11:1475-1489.
3. Gorski, K., M. Carneiro and U. Schibler. 1986. Tissue specific in vitro transcription from the mouse albumin promoter. Cell 47:767-776.
4. Lavery, D.J. and U. Schibler. 1991. Circadian transcription of the cholesterol 7α hydroxylase gene may involve the liver-enriched bZIP protein DBP. Genes Dev. 7:1871-1884.
5. nd ed). CSH Laboratory Press, Cold Spring Harbor, NY.
6. Schwartz, D.A. and D.T. Kurtz. 1996. Sequence requirements for secondary glucocorticoid inducibility of rat alpha 2u globulin genes. Mol. Cell. Endocrinol. 120:153-159.