Repression of the Escherichia coli melR promoter by MelR: Evidence that efficient repression requires the formation of a repression loop

Molecular Microbiology

Volumn 36, Issue 1, 2000, Pages 223-229

  Wade, Joseph T ^a   Belyaeva, Tamara A ^a   Hyde, Eva I ^a   Busby, Stephen J W ^a  

^a UNIVERSITY OF BIRMINGHAM   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL PROTEIN; MELIBIOSE; TRANSCRIPTION FACTOR;

ARTICLE; AUTOREGULATION; BACTERIAL GENE; BINDING SITE; CONTROLLED STUDY; DOWN REGULATION; ESCHERICHIA COLI; GENE ACTIVATION; GENE REPRESSION; NONHUMAN; NUCLEOTIDE SEQUENCE; OPERON; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN DNA BINDING; TRANSCRIPTION INITIATION;

BINDING SITES; DNA-BINDING PROTEINS; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; GENE EXPRESSION REGULATION, BACTERIAL; MELIBIOSE; MODELS, GENETIC; OPERATOR REGIONS (GENETICS); PROMOTER REGIONS (GENETICS); PROTEIN BINDING; TRANS-ACTIVATORS; TRANSCRIPTION, GENETIC;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; NEGIBACTERIA;

EID: 0034115895     PISSN: 0950382X     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2958.2000.01850.x     Document Type: Article

References (16)

1. Belyaeva, T., Wade, J., Webster, C., Howard, V., Thomas, M., Hyde, E., et al. (2000) Transcription activation at the Escherichia coli melAB promoter: the role of MelR and the cyclic AMP receptor protein. Mol Microbiol 36: 211-222.
2. Caswell, R., Webster, C., and Busby, S. (1992) Studies on the binding of the E coli MelR transcription activator protein to operator sequences at the melAB promoter. Biochem J 287: 501-508.
3. Choy, H., and Adhya, S. (1996) Negative control. In Escherichia Coli and Salmonella, Vol 1. Neidhardt, F. (ed. ). Washington: American Society for Microbiology Press, pp. 1287-1299.
4. Gallegos, M.-T., Schleif, R., Bairoch, A., Hofmann, K., and Ramos, J.-L. (1997) AraC/XylS family of transcriptional regulators. Microbiol Mol Biol Rev 61: 393-410.
5. Gostick, D., Green, J., Irvine, A., Gasson, M., and Guest, J. (1998) A novel regulatory switch mediated by the FNR-like protein of Lactobacillus casei. Microbiology 144: 705-717.
6. Gralla, J., and Collado-Vides, J. (1996) Organization and function of transcription regulatory elements. In Escherichia coli and Salmonella, Vol 1. Neidhardt, F. (ed.). Washington: American Society for Microbiology Press, pp. 1332-1245.
7. Kelsall, A., Evans, C., and Busby, S. (1985) A plasmid vector that allows fusion of the E. coli galactokinase gene to the translation standpoint of other genes. FEBS Lett 180: 155-159.
8. Lodge, J., Fear, J., Busby, S., Gunasekaran, P., and Kamini, N.-R. (1992) Broad host-range plasmids carrying the E. coli lactose and galactose operons. FEMS Lett 95: 271 -276.
9. Miller, J. (1972) Experiments in Molecular Genetics. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press.
10. Muller-Hill, B. (1998) Some repressers of bacterial transcription. Curr Opinion Microbiol 1: 145-151.
11. Schleif, R. (1996) Two positively regulated systems, ara and mal. In Escherichia coli and Salmonella, Vol 1. Neidhardt, F. (ed.). Washington: American Society for Microbiology Press, pp. 1300-1309.
12. Webster, C., Gardner, L, and Busby, S. (1989) The E. coli melR gene encodes a DNA binding protein with affinity for specific sequences located in the melibiose-operon regulatory region. Gene 83: 207-213.
13. Webster, C., Gaston, K., and Busby, S. (1988) Transcription from the E. coli melR promoter is dependent on the cyclic AMP receptor protein. Gene 68: 297-305.
14. Webster, C., Kempsell, K., Booth, I., and Busby, S. (1987) Organisation of the regulatory region of the Escherichia coli melibiose operon. Gene 59: 253-263.
15. Williams, J., Michán, C., Webster, C., and Busby, S. (1994) Interactions between the E coli MelR transcription activator protein and operator sequences at the melAB promoter. Biochem J 300: 757-763.
16. Zhou, Y., Zhang, X., and Ebright, R. (1991) Random mutagenesis of gene-sized molecules by use of PCR with Taq DNA polymerase. Nucleic Acids Res 19: 6052.