Mutants provide evidence of the importance of glycosydic chains in the activation of lipase 1 from Candida rugosa

Protein Science

Volumn 9, Issue 5, 2000, Pages 985-990

  Brocca, Stefania ^a   Alberghina, Lilia ^a   Lotti, Marina ^a   Persson, Mattias ^b   Wehtje, Ernst ^b   Adlercreutz, Patrick ^b  

^a UNIVERSITY OF MILANO BICOCCA   (Italy)

^b LUND UNIVERSITY   (Sweden)

Author keywords

Candida rugosa; Glycosylation sites; Pichia pastoris; Recombinant lipase; Site directed mutagenesis; Synthetic gene; Thermostability; Water activity profiles

Indexed keywords

FUNGAL ENZYME; LIPASE 1; UNCLASSIFIED DRUG;

ARTICLE; CANDIDA RUGOSA; DEGLYCOSYLATION; ENZYME ACTIVATION; ENZYME ACTIVITY; ESTERIFICATION; HYDROLYSIS; PRIORITY JOURNAL; SEQUENCE ANALYSIS; SITE DIRECTED MUTAGENESIS; THERMOSTABILITY;

ASPARAGINE; BLOTTING, WESTERN; CANDIDA; ENZYME ACTIVATION; GLUTAMINE; GLYCOSIDES; GLYCOSYLATION; HYDROGEN-ION CONCENTRATION; LIPASE; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; PICHIA; PLASMIDS; RECOMBINANT PROTEINS; TEMPERATURE; WATER;

CANDIDA RUGOSA; PICHIA PASTORIS;

EID: 0034114845     PISSN: 09618368     EISSN: None     Source Type: Journal    
DOI: 10.1110/ps.9.5.985     Document Type: Article

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