pH-induced conformational changes in Clostridium difficile toxin B

Infection and Immunity

Volumn 68, Issue 5, 2000, Pages 2470-2474

  Qa'dan, Maen ^a   Spyres, Lea M ^a   Ballard, Jimmy D ^a  

^a UNIVERSITY OF OKLAHOMA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CLOSTRIDIUM TOXIN;

ACIDIFICATION; ANIMAL CELL; ARTICLE; CLOSTRIDIUM DIFFICILE; CONFORMATIONAL TRANSITION; CYTOPATHOGENIC EFFECT; FLUORESCENCE; NONHUMAN; PH; PRIORITY JOURNAL; PROTEIN CONFORMATION; STAPHYLOCOCCUS AUREUS; TOXIN STRUCTURE;

ANIMALS; ANTI-BACTERIAL AGENTS; BACTERIAL PROTEINS; BACTERIAL TOXINS; CHO CELLS; CLOSTRIDIUM DIFFICILE; CRICETINAE; CYTOTOXINS; ENZYME INHIBITORS; FLUORESCENT DYES; HYDROGEN-ION CONCENTRATION; MACROLIDES; NAPHTHALENESULFONATES; PROTEIN CONFORMATION; PROTON-TRANSLOCATING ATPASES; SERINE ENDOPEPTIDASES; TRYPTOPHAN;

EID: 0034114105     PISSN: 00199567     EISSN: None     Source Type: Journal    
DOI: 10.1128/IAI.68.5.2470-2474.2000     Document Type: Article

References (15)

1. Aktories, K. 1917. Bacterial toxins that target Rho proteins. J. Clin. Investig. 99:827-829.
2. Ayyagari, A., P. Sharma, T. Venkateswarlu, S. Mehta, and K. C. Agarwal. 1986. Prevalence of Clostridium difficile in pseudomembranous and antibiotic-associated colitis in north India. J. Diarrhoeal Dis. Res. 4:157-160.
3. Ball, D. W., R. L. Van Tassell, M. D. Roberts, P. E. Hahn, D. M. Lyerly, and T. D. Wilkins. 1993. Purification and characterization of alpha-toxin produced by Clostridium novyi type A. Infect. Immun. 61:2912-2918.
4. Boquet, P., P. Munro, C. Fiorentini, and I. Just. 1998. Toxins from anaerobic-bacteria: specificity and molecular mechanisms of action. Curr. Opin. Microbiol. 1:66-74.
5. Collier, R. J. 1999. Mechanism of membrane translocation by anthrax toxin: insertion and pore formation by protective antigen. J. Appl. Microbiol. 87:283.
6. Drapeau, G. R., Y. Boily, and J. Houmard. 1972. Purification and properties of an extracellular protease of Staphylococcus aureus. J. Biol. Chem. 247: 6720-6726.
7. Florin, I., and M. Thelestam. 1983. Internalization of Clostridium difficile cytotoxin into cultured human lung fibroblasts. Biochim. Biophys. Acta 763: 383-392.
8. Florin, I., and M. Thelestam. 1986. Lysosomal involvement in cellular intoxication with Clostridium difficile toxin B. Microh. Pathog. 1:373-385.
9. Houmard, J., and G. R. Drapeau. 1972. Staphylococcal protease: a proteolytic enzyme specific for glutamoyl bonds. Proc. Natl. Acad. Sci. USA 69: 3506-3509.
10. London, E. 1992. Diphtheria toxin:membrane interaction and membrane translocation. Biochim. Biophys. Acta 1113:25-51.
11. Meador, J. D., and R. K. Tweten. 1988. Purification and characterization of toxin B from Clostridium difficile. Infect. Immun. 56:1708-1714.
12. Menard, A., K. Altendorf, D. Breves, M. Mock, and C. Montecucco. 1996. The vacuolar ATPase proton pump is required for the cytotoxicity of Bacillus anthracis lethal toxin. FEBS Lett. 386:161-164.
13. Pothoulakis, C., L. M. Barone, R. Ely, B. Faris, M. E. Clark, C. Franzblau, and J. T. LaMont. 1986. Purification and properties of Clostridium difficile cytotoxin B. J. Biol. Chem. 261:1316-1321.
14. Shoshan, M. C., T. Bergman, M. Thelestam, and I. Florin. 1993. Dithiothreitol generates an activated 250,000 mol. wt form of Clostridium difficile toxin B. Toxicon 31:845-852.
15. Sullivan, N. M., S. Pellett, and T. D. Wilkins. 1982. Purification and characterization of toxins A and B of Clostridium difficile. Infect. Immun. 35: 1032-1040.