Modified CTAB protocol using a silica matrix for isolation of plant genomic DNA

BioTechniques

Volumn 28, Issue 3, 2000, Pages 432-434

  Huang, Junchao ^a   Ge, Xuejun ^a   Sun, Mei ^a  

^a UNIVERSITY OF HONG KONG   (Hong Kong)

Author keywords

[No Author keywords available]

Indexed keywords

CESIUM CHLORIDE; DNA; SILICON DIOXIDE;

ANALYTIC METHOD; ARTICLE; DENSITY GRADIENT; DNA BINDING; DNA SEQUENCE; GENE AMPLIFICATION; GENE ISOLATION; PH; PLANT; POLYMERASE CHAIN REACTION;

EMBRYOPHYTA;

EID: 0034107707     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/00283bm08     Document Type: Article

References (6)

1. Doyle, J.J. and J.L. Doyle. 1987. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemical Bulletin 19:11-15.
2. Hoss, M. and S. Paabo. 1993. DNA extraction from Pleistocene bones by a silica-based purification method. Nucleic Acids Res. 21:3913-3914.
3. Huang, J.C. and M. Sun. Genetic diversity and relationships of sweet potato and its wild relatives in Ipomoea series Batatas (Convolvulaceae) as revealed by inter-simple sequence repeat (ISSR) and restriction analysis of chloroplast DNA. Theor. Appl. Genet. (In press).
4. Rogers, S.O. and A.J. Bendich. 1985. Extraction of DNA from milligram amounts of fresh, herbarium, and mummified plant tissues. Plant Mol. Bio. 5:69-76.
5. Vogelstein, B. and D. Gillespie. 1979. Preparative and analytical purification of DNA from agarose. Proc. Natl. Acad. Sci. USA 76:615-619.
6. Ziegenhagen, B., P. Guillemaut and F. Scholz. 1993. A procedure for mini-preparation of genomic DNA from needles of silver fir (Abies alba Mill.). Plant Mol. Biol. Reporter11:117-121.