Evaluation of automated RNA-extraction technology and a qualitative HCV assay for sensitivity and detection of HCV RNA in pool-screening systems

Transfusion

Volumn 40, Issue 5, 2000, Pages 575-579

  Beld, Marcel ^a   Habibuw, M R ^b   Rebers, S P H ^b   Boom, R ^b   Reesink, H W ^b  

^a UNIVERSITY OF AMSTERDAM   (Netherlands)

^b NONE

Author keywords

[No Author keywords available]

Indexed keywords

RNA;

ARTICLE; EXTRACTION; GENE AMPLIFICATION; HEPATITIS C; POLYMERASE CHAIN REACTION; VIRUS CHARACTERIZATION; WORLD HEALTH ORGANIZATION;

AUTOANALYSIS; BLOOD TRANSFUSION; DISEASE TRANSMISSION; EVALUATION STUDIES; HEPACIVIRUS; HUMANS; REFERENCE STANDARDS; RNA, VIRAL; SENSITIVITY AND SPECIFICITY;

EID: 0034107517     PISSN: 00411132     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1537-2995.2000.40050575.x     Document Type: Article

References (15)

1. Schreiber GB, Busch MP, Kleinman SH, Korelitz JJ. The risk of transfusion-transmitted viral infections. N Engl J Med 1996;334:1685-90.
2. Alter HJ. To C or not to C: these are the questions. Blood 1995;85:1681-95.
3. Roggendorf M, Lu M, Meisel H, et al. Rational use of diagnostic tools in hepatitis C. J Hepatol 1996;24:26-34.
4. Widell A, Elmud H, Persson MH, Jonsson M. Transmission of hepatitis C via both erythrocyte and platelet transfusions from a single donor in serological window-phase of hepatitis C. Vox Sang 1996;71:55-7.
5. Beld M, Penning M, van Putten M, et al. Quantitative antibody responses to structural (Core) and nonstructural (NS3, NS4, and NS5) hepatitis C virus proteins among seroconverting injecting drug users: impact of epitope variation and relationship to detection of HCV RNA in blood. Hepatology 1999:29:1288-98.
6. Rogers PM, Saldanha J, Allain JP. Report of EPFA/NIBSC Workshop "Nucleic Acid Amplification Test (NAT) for the detection of blood-borne viruses" held on 31 October 1996 in Amsterdam, the Netherlands. Vox Sang 1996;72:199-206.
7. Nübling CM, Seitz R, Löwer J. Application of nucleic acid amplification techniques for blood donation screening. Infusions therapie 1998;25:86-90.
8. Boom R, Sol CJ, Salimans MM, et al. Rapid and simple method for purification of nucleic acids. J Clin Microbiol 1990;28:495-503.
9. Van Buul C, Cuypers H, Lelie P, et al. The NucliSense™ Extractor for automated nucleic acid isolation. Infusionstherapie 1998;25:147-51.
10. DiDomenico N, Link H, Knobel R, et al. COBAS AMPLICOR™: fully automated RNA and DNA amplification and detection system for routine diagnostic PCR. Clin Chem 1996;42:1915-23.
11. Damen M, Cuypers HT, Zaaijer HL, et al. International collaborative study on the second EUROHEP HCV RNA reference panel. J Virol Methods 1996;58:175-85.
12. Saldanha J, Minor P. Collaborative study to assess the suitability of an HCV-RNA reference sample for detection of an HCV-RNA in plasma pools by PCR. Vox Sang 1996;70:148-51.
13. Simmonds P, McOmish F, Yap PL, et al. Sequence variability in the 5′ non-coding region of hepatitis C virus: identification of a new virus type and restrictions on sequence diversity. J Gen Virol 1993;74:661-8.
14. Bukh J, Purcell RH, Miller RH. Sequence analysis of the 5′ noncoding region of hepatitis C virus. Proc Natl Acad Sci USA 1992;89:4942-6.
15. Simmonds P, Holmes EC, Cha TA, et al. Classification of hepatitis C virus into six major genotypes and a series of subtypes by phylogenetic analysis of the NS-5 region. I Gen Virol 1993;74:2391-9.