Morphological and cytological characteristics of somatic hybrids of Nicotiana tabacum L. (+) N. megalosiphon Heurk. et Mull.

In Vitro Cellular and Developmental Biology - Plant

Volumn 36, Issue 1, 2000, Pages 69-73

  Ilcheva, V ^a   San, L H ^b   Dimitrov, B ^a   Zagorska, N ^a  

^a INSTITUTE OF PHYSIOLOGY   (Bulgaria)

^b Seita   (France)

Author keywords

Flow cytometry; Ploidy; Protoplast fusion; Tobacco

Indexed keywords

CALLUS CULTURE; CELL HYBRIDIZATION; DIPLOIDY; GENETIC DIVERSITY; GENETIC SELECTION; HYBRID; HYBRID CELL; NICOTIANA; PESTICIDE RESISTANCE; PHENOTYPE; PLANT GENOME; TETRAPLOIDY;

NICOTIANA MEGALOSIPHON; NICOTIANA TABACUM;

EID: 0034106575     PISSN: 10545476     EISSN: None     Source Type: Journal    
DOI: 10.1007/s11627-000-0016-4     Document Type: Article

References (22)

1. Bajaj, Y. P. S. Somatic hybridization - a rich source of genetic variability. In: Bajaj, Y. P. S., ed. Biotechnology in agriculture and forestry, vol. 27. New York: Springer Verlag; 1994:3-32.
2. De Vries, S. E.; Ferwerda, M. A.; Leonen, A. E. H. M.; Pijnacker L. P.; Feenstra, W. J. Chromosomes in somatic hybrids between Nicotiana plumbaginifolia and monoploid potato. Theor. Appl. Genet. 75:170-176; 1987.
3. Dominique, M.; Spencer, B. C. A cytometric exercise in plant DNA histograms with 2C values for 70 species. Biol Cell. 78:41-45; 1993.
4. Donaldson, P.; Sproule, A.; Bevis, E.; Pandeya, R.; Keller, W. A.; Gleddie, S. Non-random chloroplast segregation in N. tabacum (+) N. rustica somatic hybrids selected by dual nuclear-encoded resistance. Theor. Appl. Genet. 86:465-473; 1993.
5. Famelaer, I.; Gleba, Y. Y.; Sidorov, V. A.; Kaleda, V. A.; Parokonny, A. S.; Boryshuk, N. V.; Cherep, N. N.; Negrutiu, I.; Jacobs, M. Intergeneric asymmetric hybrids between Nicotiana plumbuginifolia and Nicotiana sylvestris obtained by "gamma-fusion". Plant Sci. 61:105-117; 1989.
6. Gleba, Y. Y.; Shlumukov, L. R. Somatic hybridization and eybridization. In: Bhojwani, S. S., ed. Development of crop science 19. Plant tissue culture: applications and limitations. Amsterdam: Elsevier; 1990:316-344.
7. Heller, R. Recherches sur la nutrition minérale des tissus végétaux cultivés in vitro. Ann. Sci. Nat. Bot. Biol. Végét. 14:1-223; 1953.
8. Hinnisdaels, S.; Bariller, L.; Mouras, A.; Sidorov, V.; Del-Favero, J.; Veuskens, J.; Negrutiu, I.; Jacobs, M. Highly asymmetric intergeneric nuclear hybrids between Nicotiana and Petunia: evidence for recombinations and translocation events in somatic hybrid plants after "gamma"-fusion N. tabacum. Theor. Appl. Genet. 82:609-614; 1991a.
9. Hinnisdaels, S.; Charti-Chhetri, G. B.; Negritiu, I. Somatic hybridization. Protoplast fusion - the PEG system. In: Negritiu, I.; Charti-Chhetri, G. B., eds. BioMethods. A laboratory guide for cellular and molecular plant biology, vol. 4. Basel, Boston, Berlin: Birkhäuser Verlag; 1991b:32-39.
10. Hinnisdaels, S.; Mouras, A.; Salesses, G.; Veuskens, J.; Taylor, C.; Charti-Chetri, G. B.; Negrutiu, I.; Jacobs, M. Translocation events demonstrated by molecular, in situ hybridization and chromosome pairing analyses in highly asymmetric somatic hybrid plants. Transgenic Res. 1:170-176; 1992.
11. Hranov, A. Some problems on crossability between N. tabacum L. and N. megalosiphon Heurk et Müll. Bulgarian Tobacco 10:45-48; 1970.
12. Manolov, A. Transfer of complex resistance to blue mold and powdery mildew. Bulgarian Tobacco 12:9-17; 1981.
13. Morel, G. Sur la développement de tissus de vignes cultivées in vitro. C. R. Acad. Sci. Soc. Biol. Paris. 138:62; 1944.
14. Murashige, T.; Skoog, F. A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol. Plant. 15:473-497; 1962.
15. Palakarcheva, M. Interspecific hybridization in g. Nicotiana. Sofia: Bulgarian Academy of Sciences Publishing House; 1988.
16. Pelletier, G. Somatic hybridization. In: Hayward, M. D.; Bosemark, N. O.; Romagosa, I., eds. Plant breeding: principles and prospects. London: Chapman & Hall; 1993:93-106.
17. Piastuch, W. C.; Bates, G. W. Chromosomal analysis of Nicotiana asymmetric somatic hybrids by dot blotting and in situ hybridisation. Mol. Gen. Genet. 222:97-103; 1990.
18. Sproule, A.; Donaldson, P.; Dijak, M.; Bevis, E.; Pandeya, R.; Keller, W. A.; Gleddie, S. Fertile somatic hybrids between transgenic Nicotiana tabacum and transgenic Nicotiana debneyi selected by dual antibiotic resistance. Theor. Appl. Genet. 82:450-456; 1991.
19. Stavely, J. R. Disease resistance. Nicotiana: Proceeding for experimental use. U.S. Department of Agriculture 1:87-110; 1979.
20. Vos, P.; Hogers, R.; Bleeker, M.; Reijans, M.; van de Lee, T.; Homes, M.; Frijters, A.; Pot, J.; Peleman, J.; Kupler, M.; Zabeau, M. AFLP: a new technique for DNA fingerprinting. Nucleic Acids Res. 25:4407-4414; 1995.
21. Zelcer, A.; Aviv, D.; Galun, E. Interspecific transfer of cytoplasmic male sterilility by fusion between protoplasts of normal Nicotiana sylvestris and X-irradiated protoplasts of male sterile N. tabacum. Z. Pflanzenphysiol. 90:397-407; 1978.
22. Zhu, X.Y.; Negrutiu, I. Isolation and culture of protoplasts. In: Negritiu, I.; Charti-Chhetri, G. B., eds. BioMethods. A laboratory guide for cellular and molecular plant biology. Basel, Boston, Berlin: Birkhäuser Verlag; 1991:18-27.