Membrane-bound human 3β-hydroxysteroid dehydrogenase: Overexpression with His-tag using a baculovirus system and single-step purification

Protein Expression and Purification

Volumn 18, Issue 2, 2000, Pages 169-174

  Huang, Yi Wei ^a   Lu, Ming Liang ^a   Qi, Heng ^a   Lin, Sheng Xiang ^a  

^a LAVAL UNIVERSITY   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

3BETA HYDROXYSTEROID DEHYDROGENASE 1; AFFINITY CHROMATOGRAPHY; BACULOVIRUS EXPRESSION SYSTEM; CHELATION; DETERGENT; ENZYME STRUCTURE; ENZYME SUBSTRATE COMPLEX; GENE OVEREXPRESSION; PRASTERONE; PROTEIN PURIFICATION; SOLUBILIZATION; STRUCTURE ACTIVITY RELATION;

UNIDENTIFIED BACULOVIRUS;

EID: 0034105490     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1006/prep.1999.1180     Document Type: Article

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