Making colony PCR easier by adding gel-loading buffer to the amplification reaction

BioTechniques

Volumn 28, Issue 3, 2000, Pages 424-426

  Menossi, Marcelo ^a   Cremonese Jr , Nilson ^a   Maron, Lyza G ^a   Arruda, Paulo ^a  

^a UNIVERSITY OF CAMPINAS   (Brazil)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIUM LYSATE; BUFFER;

ARTICLE; BACTERIUM COLONY; GEL; GENE AMPLIFICATION; GENE TARGETING; PLASMID; POLYMERASE CHAIN REACTION;

BACTERIA (MICROORGANISMS);

EID: 0034099828     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/00283bm06     Document Type: Article

References (7)

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4. Menossi, M., J.A. Martínez-Izquierdo and P. Puigdomènech. 1997. Tissue specific activity and hormone control of the promoter of the gene coding for a maize hydroxyproline-rich glycoprotein. Plant Sci. 125:189-200.
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7. Zon, L.I., D.M. Dorfman and S.H. Orkin. 1989. The polymerase chain reaction colony miniprep. BioTechniques 7:696-698.