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Volumn , Issue 6, 2000, Pages 781-783

Entrapment of lipases in hydrophobic sol-gel-materials: Efficient heterogeneous biocatalysts in aqueous medium

Author keywords

Catalysis; Enzymes; Ester hydrolysis; Heterogeneous catalysis; Sol gel

Indexed keywords

ESTER; SILANE DERIVATIVE; TRIACYLGLYCEROL LIPASE;

EID: 0034099693     PISSN: 00397881     EISSN: None     Source Type: Journal    
DOI: 10.1055/s-2000-6276     Document Type: Article
Times cited : (71)

References (26)
  • 1
    • 0003458664 scopus 로고
    • VCH: Weinheim
    • Enzyme Catalysis in Organic Synthesis: A Comprehensive Handbook, Vol. I; Drauz, K.; Waldmann, H., Eds.;VCH: Weinheim, 1995. Faber, K. Biotransformations in Organic Chemistry, 3rd ed.; Springer: Berlin, 1997. Uhlig, H. Industrial Enzymes and their Applications; Wiley: New York, 1998. Biocatalysts for Fine Chemicals Synthesis; Roberts, S. M., Ed.; Wiley: New York, 1999.
    • (1995) Enzyme Catalysis in Organic Synthesis: A Comprehensive Handbook , vol.1
    • Drauz, K.1    Waldmann, H.2
  • 2
    • 0003479152 scopus 로고    scopus 로고
    • Springer: Berlin
    • Enzyme Catalysis in Organic Synthesis: A Comprehensive Handbook, Vol. I; Drauz, K.; Waldmann, H., Eds.;VCH: Weinheim, 1995. Faber, K. Biotransformations in Organic Chemistry, 3rd ed.; Springer: Berlin, 1997. Uhlig, H. Industrial Enzymes and their Applications; Wiley: New York, 1998. Biocatalysts for Fine Chemicals Synthesis; Roberts, S. M., Ed.; Wiley: New York, 1999.
    • (1997) Biotransformations in Organic Chemistry, 3rd Ed.
    • Faber, K.1
  • 3
    • 0003854608 scopus 로고    scopus 로고
    • Wiley: New York
    • Enzyme Catalysis in Organic Synthesis: A Comprehensive Handbook, Vol. I; Drauz, K.; Waldmann, H., Eds.;VCH: Weinheim, 1995. Faber, K. Biotransformations in Organic Chemistry, 3rd ed.; Springer: Berlin, 1997. Uhlig, H. Industrial Enzymes and their Applications; Wiley: New York, 1998. Biocatalysts for Fine Chemicals Synthesis; Roberts, S. M., Ed.; Wiley: New York, 1999.
    • (1998) Industrial Enzymes and Their Applications
    • Uhlig, H.1
  • 4
    • 0003804449 scopus 로고    scopus 로고
    • Wiley: New York
    • Enzyme Catalysis in Organic Synthesis: A Comprehensive Handbook, Vol. I; Drauz, K.; Waldmann, H., Eds.;VCH: Weinheim, 1995. Faber, K. Biotransformations in Organic Chemistry, 3rd ed.; Springer: Berlin, 1997. Uhlig, H. Industrial Enzymes and their Applications; Wiley: New York, 1998. Biocatalysts for Fine Chemicals Synthesis; Roberts, S. M., Ed.; Wiley: New York, 1999.
    • (1999) Biocatalysts for Fine Chemicals Synthesis
    • Roberts, S.M.1
  • 9
    • 0031251298 scopus 로고    scopus 로고
    • Review of hydrophobic alkyl-modified silicon oxides as matrices for the entrapment of enzymes
    • Review of Hydrophobic alkyl-modified silicon oxides as matrices for the entrapment of enzymes: Reetz, M. T. Adv. Mater. (Weinheim, Ger.) 1997, 9, 943.
    • (1997) Adv. Mater. (Weinheim, Ger.) , vol.9 , pp. 943
    • Reetz, M.T.1
  • 10
    • 33751158445 scopus 로고    scopus 로고
    • 2
    • 2: Avnir, D.; Braun, S.; Lev, O.; Ottolenghi, M. Chem. Mater. 1994, 6, 1605. Avnir, D. Acc. Chem. Res. 1995, 28, 328. Livage, J. C. R. Acad. Sci., Ser. IIb: Mec., Phys. Chim. Astron. 1996, 322, 417.
    • (1994) Chem. Mater. , vol.6 , pp. 1605
    • Avnir, D.1    Braun, S.2    Lev, O.3    Ottolenghi, M.4
  • 11
    • 0000324041 scopus 로고
    • 2: Avnir, D.; Braun, S.; Lev, O.; Ottolenghi, M. Chem. Mater. 1994, 6, 1605. Avnir, D. Acc. Chem. Res. 1995, 28, 328. Livage, J. C. R. Acad. Sci., Ser. IIb: Mec., Phys. Chim. Astron. 1996, 322, 417.
    • (1995) Acc. Chem. Res. , vol.28 , pp. 328
    • Avnir, D.1
  • 13
    • 0031023666 scopus 로고    scopus 로고
    • Verger, R. Trends Biotechnol. 1997, 15, 32. Nitsch, W.; Maksymiw, R.; Erdmann, H. J. Colloid Interface Sci. 1991, 141, 322. Lipases: Their Structure, Biochemistry and Application; Woolley, P.; Petersen, S. B., Eds.; Cambridge University Press: Cambridge, 1994. Schrag, J. D.; Li, Y.; Wu, S.; Cygler, M. Nature (London) 1991, 351, 761. van Tilheurgh, H.; Egloff, M. -P.; Martinez, C.; Rugani, N.; Verger R.; Cambillau, C. Nature (London) 1993, 362, 814. Martinez, C.; De Geus, P.; Lauwereys, M.; Matthyssens G.; Cambillau, C. Nature (London) 1992, 356, 615. Schmid, R. D.; Verger, R. Angew. Chem., Int. Ed Engl. 1998, 37, 1608. Kazlauskas, R. J. Trends Biotechnol. 1994, 12, 464. Jaeger, K. -E.; Ransac, S.; Dijkstra, B. W.; Colson, C.; van Heuvel, M; Misset, O. FEMS Microbiol. Rev. 1994, 15, 29. Brzozowski, A. M.; Derewenda, U.; Derewenda, Z. S.; Dodson, G. G.; Lawson, D. M.; Turkenburg, J. P.; Bjorkling, F.; Huge-Jensen, B.; Patkar; S. A.; Thim, L. Nature (London) 1991, 351, 491. Louwrier, A.; Drtina, G. J.; Klibanov, A. M. Biotechnol. Bioeng. 1996, 50, 1.
    • (1997) Trends Biotechnol. , vol.15 , pp. 32
    • Verger, R.1
  • 14
    • 0026104742 scopus 로고
    • Verger, R. Trends Biotechnol. 1997, 15, 32. Nitsch, W.; Maksymiw, R.; Erdmann, H. J. Colloid Interface Sci. 1991, 141, 322. Lipases: Their Structure, Biochemistry and Application; Woolley, P.; Petersen, S. B., Eds.; Cambridge University Press: Cambridge, 1994. Schrag, J. D.; Li, Y.; Wu, S.; Cygler, M. Nature (London) 1991, 351, 761. van Tilheurgh, H.; Egloff, M. -P.; Martinez, C.; Rugani, N.; Verger R.; Cambillau, C. Nature (London) 1993, 362, 814. Martinez, C.; De Geus, P.; Lauwereys, M.; Matthyssens G.; Cambillau, C. Nature (London) 1992, 356, 615. Schmid, R. D.; Verger, R. Angew. Chem., Int. Ed Engl. 1998, 37, 1608. Kazlauskas, R. J. Trends Biotechnol. 1994, 12, 464. Jaeger, K. -E.; Ransac, S.; Dijkstra, B. W.; Colson, C.; van Heuvel, M; Misset, O. FEMS Microbiol. Rev. 1994, 15, 29. Brzozowski, A. M.; Derewenda, U.; Derewenda, Z. S.; Dodson, G. G.; Lawson, D. M.; Turkenburg, J. P.; Bjorkling, F.; Huge-Jensen, B.; Patkar; S. A.; Thim, L. Nature (London) 1991, 351, 491. Louwrier, A.; Drtina, G. J.; Klibanov, A. M. Biotechnol. Bioeng. 1996, 50, 1.
    • (1991) J. Colloid Interface Sci. , vol.141 , pp. 322
    • Nitsch, W.1    Maksymiw, R.2    Erdmann, H.3
  • 15
    • 0003740747 scopus 로고
    • Cambridge University Press: Cambridge
    • Verger, R. Trends Biotechnol. 1997, 15, 32. Nitsch, W.; Maksymiw, R.; Erdmann, H. J. Colloid Interface Sci. 1991, 141, 322. Lipases: Their Structure, Biochemistry and Application; Woolley, P.; Petersen, S. B., Eds.; Cambridge University Press: Cambridge, 1994. Schrag, J. D.; Li, Y.; Wu, S.; Cygler, M. Nature (London) 1991, 351, 761. van Tilheurgh, H.; Egloff, M. -P.; Martinez, C.; Rugani, N.; Verger R.; Cambillau, C. Nature (London) 1993, 362, 814. Martinez, C.; De Geus, P.; Lauwereys, M.; Matthyssens G.; Cambillau, C. Nature (London) 1992, 356, 615. Schmid, R. D.; Verger, R. Angew. Chem., Int. Ed Engl. 1998, 37, 1608. Kazlauskas, R. J. Trends Biotechnol. 1994, 12, 464. Jaeger, K. -E.; Ransac, S.; Dijkstra, B. W.; Colson, C.; van Heuvel, M; Misset, O. FEMS Microbiol. Rev. 1994, 15, 29. Brzozowski, A. M.; Derewenda, U.; Derewenda, Z. S.; Dodson, G. G.; Lawson, D. M.; Turkenburg, J. P.; Bjorkling, F.; Huge-Jensen, B.; Patkar; S. A.; Thim, L. Nature (London) 1991, 351, 491. Louwrier, A.; Drtina, G. J.; Klibanov, A. M. Biotechnol. Bioeng. 1996, 50, 1.
    • (1994) Lipases: Their Structure, Biochemistry and Application
    • Woolley, P.1    Petersen, S.B.2
  • 16
    • 0026432669 scopus 로고
    • Verger, R. Trends Biotechnol. 1997, 15, 32. Nitsch, W.; Maksymiw, R.; Erdmann, H. J. Colloid Interface Sci. 1991, 141, 322. Lipases: Their Structure, Biochemistry and Application; Woolley, P.; Petersen, S. B., Eds.; Cambridge University Press: Cambridge, 1994. Schrag, J. D.; Li, Y.; Wu, S.; Cygler, M. Nature (London) 1991, 351, 761. van Tilheurgh, H.; Egloff, M. -P.; Martinez, C.; Rugani, N.; Verger R.; Cambillau, C. Nature (London) 1993, 362, 814. Martinez, C.; De Geus, P.; Lauwereys, M.; Matthyssens G.; Cambillau, C. Nature (London) 1992, 356, 615. Schmid, R. D.; Verger, R. Angew. Chem., Int. Ed Engl. 1998, 37, 1608. Kazlauskas, R. J. Trends Biotechnol. 1994, 12, 464. Jaeger, K. -E.; Ransac, S.; Dijkstra, B. W.; Colson, C.; van Heuvel, M; Misset, O. FEMS Microbiol. Rev. 1994, 15, 29. Brzozowski, A. M.; Derewenda, U.; Derewenda, Z. S.; Dodson, G. G.; Lawson, D. M.; Turkenburg, J. P.; Bjorkling, F.; Huge-Jensen, B.; Patkar; S. A.; Thim, L. Nature (London) 1991, 351, 491. Louwrier, A.; Drtina, G. J.; Klibanov, A. M. Biotechnol. Bioeng. 1996, 50, 1.
    • (1991) Nature (London) , vol.351 , pp. 761
    • Schrag, J.D.1    Li, Y.2    Wu, S.3    Cygler, M.4
  • 17
    • 0027200087 scopus 로고
    • Verger, R. Trends Biotechnol. 1997, 15, 32. Nitsch, W.; Maksymiw, R.; Erdmann, H. J. Colloid Interface Sci. 1991, 141, 322. Lipases: Their Structure, Biochemistry and Application; Woolley, P.; Petersen, S. B., Eds.; Cambridge University Press: Cambridge, 1994. Schrag, J. D.; Li, Y.; Wu, S.; Cygler, M. Nature (London) 1991, 351, 761. van Tilheurgh, H.; Egloff, M. -P.; Martinez, C.; Rugani, N.; Verger R.; Cambillau, C. Nature (London) 1993, 362, 814. Martinez, C.; De Geus, P.; Lauwereys, M.; Matthyssens G.; Cambillau, C. Nature (London) 1992, 356, 615. Schmid, R. D.; Verger, R. Angew. Chem., Int. Ed Engl. 1998, 37, 1608. Kazlauskas, R. J. Trends Biotechnol. 1994, 12, 464. Jaeger, K. -E.; Ransac, S.; Dijkstra, B. W.; Colson, C.; van Heuvel, M; Misset, O. FEMS Microbiol. Rev. 1994, 15, 29. Brzozowski, A. M.; Derewenda, U.; Derewenda, Z. S.; Dodson, G. G.; Lawson, D. M.; Turkenburg, J. P.; Bjorkling, F.; Huge-Jensen, B.; Patkar; S. A.; Thim, L. Nature (London) 1991, 351, 491. Louwrier, A.; Drtina, G. J.; Klibanov, A. M. Biotechnol. Bioeng. 1996, 50, 1.
    • (1993) Nature (London) , vol.362 , pp. 814
    • Van Tilheurgh, H.1    Egloff, M.-P.2    Martinez, C.3    Rugani, N.4    Verger, R.5    Cambillau, C.6
  • 18
    • 0026583770 scopus 로고
    • Verger, R. Trends Biotechnol. 1997, 15, 32. Nitsch, W.; Maksymiw, R.; Erdmann, H. J. Colloid Interface Sci. 1991, 141, 322. Lipases: Their Structure, Biochemistry and Application; Woolley, P.; Petersen, S. B., Eds.; Cambridge University Press: Cambridge, 1994. Schrag, J. D.; Li, Y.; Wu, S.; Cygler, M. Nature (London) 1991, 351, 761. van Tilheurgh, H.; Egloff, M. -P.; Martinez, C.; Rugani, N.; Verger R.; Cambillau, C. Nature (London) 1993, 362, 814. Martinez, C.; De Geus, P.; Lauwereys, M.; Matthyssens G.; Cambillau, C. Nature (London) 1992, 356, 615. Schmid, R. D.; Verger, R. Angew. Chem., Int. Ed Engl. 1998, 37, 1608. Kazlauskas, R. J. Trends Biotechnol. 1994, 12, 464. Jaeger, K. -E.; Ransac, S.; Dijkstra, B. W.; Colson, C.; van Heuvel, M; Misset, O. FEMS Microbiol. Rev. 1994, 15, 29. Brzozowski, A. M.; Derewenda, U.; Derewenda, Z. S.; Dodson, G. G.; Lawson, D. M.; Turkenburg, J. P.; Bjorkling, F.; Huge-Jensen, B.; Patkar; S. A.; Thim, L. Nature (London) 1991, 351, 491. Louwrier, A.; Drtina, G. J.; Klibanov, A. M. Biotechnol. Bioeng. 1996, 50, 1.
    • (1992) Nature (London) , vol.356 , pp. 615
    • Martinez, C.1    De Geus, P.2    Lauwereys, M.3    Matthyssens, G.4    Cambillau, C.5
  • 19
    • 0032479388 scopus 로고    scopus 로고
    • Verger, R. Trends Biotechnol. 1997, 15, 32. Nitsch, W.; Maksymiw, R.; Erdmann, H. J. Colloid Interface Sci. 1991, 141, 322. Lipases: Their Structure, Biochemistry and Application; Woolley, P.; Petersen, S. B., Eds.; Cambridge University Press: Cambridge, 1994. Schrag, J. D.; Li, Y.; Wu, S.; Cygler, M. Nature (London) 1991, 351, 761. van Tilheurgh, H.; Egloff, M. -P.; Martinez, C.; Rugani, N.; Verger R.; Cambillau, C. Nature (London) 1993, 362, 814. Martinez, C.; De Geus, P.; Lauwereys, M.; Matthyssens G.; Cambillau, C. Nature (London) 1992, 356, 615. Schmid, R. D.; Verger, R. Angew. Chem., Int. Ed Engl. 1998, 37, 1608. Kazlauskas, R. J. Trends Biotechnol. 1994, 12, 464. Jaeger, K. -E.; Ransac, S.; Dijkstra, B. W.; Colson, C.; van Heuvel, M; Misset, O. FEMS Microbiol. Rev. 1994, 15, 29. Brzozowski, A. M.; Derewenda, U.; Derewenda, Z. S.; Dodson, G. G.; Lawson, D. M.; Turkenburg, J. P.; Bjorkling, F.; Huge-Jensen, B.; Patkar; S. A.; Thim, L. Nature (London) 1991, 351, 491. Louwrier, A.; Drtina, G. J.; Klibanov, A. M. Biotechnol. Bioeng. 1996, 50, 1.
    • (1998) Angew. Chem., Int. Ed Engl. , vol.37 , pp. 1608
    • Schmid, R.D.1    Verger, R.2
  • 20
    • 0028075638 scopus 로고
    • Verger, R. Trends Biotechnol. 1997, 15, 32. Nitsch, W.; Maksymiw, R.; Erdmann, H. J. Colloid Interface Sci. 1991, 141, 322. Lipases: Their Structure, Biochemistry and Application; Woolley, P.; Petersen, S. B., Eds.; Cambridge University Press: Cambridge, 1994. Schrag, J. D.; Li, Y.; Wu, S.; Cygler, M. Nature (London) 1991, 351, 761. van Tilheurgh, H.; Egloff, M. -P.; Martinez, C.; Rugani, N.; Verger R.; Cambillau, C. Nature (London) 1993, 362, 814. Martinez, C.; De Geus, P.; Lauwereys, M.; Matthyssens G.; Cambillau, C. Nature (London) 1992, 356, 615. Schmid, R. D.; Verger, R. Angew. Chem., Int. Ed Engl. 1998, 37, 1608. Kazlauskas, R. J. Trends Biotechnol. 1994, 12, 464. Jaeger, K. -E.; Ransac, S.; Dijkstra, B. W.; Colson, C.; van Heuvel, M; Misset, O. FEMS Microbiol. Rev. 1994, 15, 29. Brzozowski, A. M.; Derewenda, U.; Derewenda, Z. S.; Dodson, G. G.; Lawson, D. M.; Turkenburg, J. P.; Bjorkling, F.; Huge-Jensen, B.; Patkar; S. A.; Thim, L. Nature (London) 1991, 351, 491. Louwrier, A.; Drtina, G. J.; Klibanov, A. M. Biotechnol. Bioeng. 1996, 50, 1.
    • (1994) Trends Biotechnol. , vol.12 , pp. 464
    • Kazlauskas, R.J.1
  • 21
    • 84982614009 scopus 로고
    • Verger, R. Trends Biotechnol. 1997, 15, 32. Nitsch, W.; Maksymiw, R.; Erdmann, H. J. Colloid Interface Sci. 1991, 141, 322. Lipases: Their Structure, Biochemistry and Application; Woolley, P.; Petersen, S. B., Eds.; Cambridge University Press: Cambridge, 1994. Schrag, J. D.; Li, Y.; Wu, S.; Cygler, M. Nature (London) 1991, 351, 761. van Tilheurgh, H.; Egloff, M. -P.; Martinez, C.; Rugani, N.; Verger R.; Cambillau, C. Nature (London) 1993, 362, 814. Martinez, C.; De Geus, P.; Lauwereys, M.; Matthyssens G.; Cambillau, C. Nature (London) 1992, 356, 615. Schmid, R. D.; Verger, R. Angew. Chem., Int. Ed Engl. 1998, 37, 1608. Kazlauskas, R. J. Trends Biotechnol. 1994, 12, 464. Jaeger, K. -E.; Ransac, S.; Dijkstra, B. W.; Colson, C.; van Heuvel, M; Misset, O. FEMS Microbiol. Rev. 1994, 15, 29. Brzozowski, A. M.; Derewenda, U.; Derewenda, Z. S.; Dodson, G. G.; Lawson, D. M.; Turkenburg, J. P.; Bjorkling, F.; Huge-Jensen, B.; Patkar; S. A.; Thim, L. Nature (London) 1991, 351, 491. Louwrier, A.; Drtina, G. J.; Klibanov, A. M. Biotechnol. Bioeng. 1996, 50, 1.
    • (1994) FEMS Microbiol. Rev. , vol.15 , pp. 29
    • Jaeger, K.-E.1    Ransac, S.2    Dijkstra, B.W.3    Colson, C.4    Van Heuvel, M.5    Misset, O.6
  • 22
    • 0026418174 scopus 로고
    • Verger, R. Trends Biotechnol. 1997, 15, 32. Nitsch, W.; Maksymiw, R.; Erdmann, H. J. Colloid Interface Sci. 1991, 141, 322. Lipases: Their Structure, Biochemistry and Application; Woolley, P.; Petersen, S. B., Eds.; Cambridge University Press: Cambridge, 1994. Schrag, J. D.; Li, Y.; Wu, S.; Cygler, M. Nature (London) 1991, 351, 761. van Tilheurgh, H.; Egloff, M. -P.; Martinez, C.; Rugani, N.; Verger R.; Cambillau, C. Nature (London) 1993, 362, 814. Martinez, C.; De Geus, P.; Lauwereys, M.; Matthyssens G.; Cambillau, C. Nature (London) 1992, 356, 615. Schmid, R. D.; Verger, R. Angew. Chem., Int. Ed Engl. 1998, 37, 1608. Kazlauskas, R. J. Trends Biotechnol. 1994, 12, 464. Jaeger, K. -E.; Ransac, S.; Dijkstra, B. W.; Colson, C.; van Heuvel, M; Misset, O. FEMS Microbiol. Rev. 1994, 15, 29. Brzozowski, A. M.; Derewenda, U.; Derewenda, Z. S.; Dodson, G. G.; Lawson, D. M.; Turkenburg, J. P.; Bjorkling, F.; Huge-Jensen, B.; Patkar; S. A.; Thim, L. Nature (London) 1991, 351, 491. Louwrier, A.; Drtina, G. J.; Klibanov, A. M. Biotechnol. Bioeng. 1996, 50, 1.
    • (1991) Nature (London) , vol.351 , pp. 491
    • Brzozowski, A.M.1    Derewenda, U.2    Derewenda, Z.S.3    Dodson, G.G.4    Lawson, D.M.5    Turkenburg, J.P.6    Bjorkling, F.7    Huge-Jensen, B.8    Patkar, S.A.9    Thim, L.10
  • 23
    • 0030570083 scopus 로고    scopus 로고
    • Verger, R. Trends Biotechnol. 1997, 15, 32. Nitsch, W.; Maksymiw, R.; Erdmann, H. J. Colloid Interface Sci. 1991, 141, 322. Lipases: Their Structure, Biochemistry and Application; Woolley, P.; Petersen, S. B., Eds.; Cambridge University Press: Cambridge, 1994. Schrag, J. D.; Li, Y.; Wu, S.; Cygler, M. Nature (London) 1991, 351, 761. van Tilheurgh, H.; Egloff, M. -P.; Martinez, C.; Rugani, N.; Verger R.; Cambillau, C. Nature (London) 1993, 362, 814. Martinez, C.; De Geus, P.; Lauwereys, M.; Matthyssens G.; Cambillau, C. Nature (London) 1992, 356, 615. Schmid, R. D.; Verger, R. Angew. Chem., Int. Ed Engl. 1998, 37, 1608. Kazlauskas, R. J. Trends Biotechnol. 1994, 12, 464. Jaeger, K. -E.; Ransac, S.; Dijkstra, B. W.; Colson, C.; van Heuvel, M; Misset, O. FEMS Microbiol. Rev. 1994, 15, 29. Brzozowski, A. M.; Derewenda, U.; Derewenda, Z. S.; Dodson, G. G.; Lawson, D. M.; Turkenburg, J. P.; Bjorkling, F.; Huge-Jensen, B.; Patkar; S. A.; Thim, L. Nature (London) 1991, 351, 491. Louwrier, A.; Drtina, G. J.; Klibanov, A. M. Biotechnol. Bioeng. 1996, 50, 1.
    • (1996) Biotechnol. Bioeng. , vol.50 , pp. 1
    • Louwrier, A.1    Drtina, G.J.2    Klibanov, A.M.3
  • 26
    • 0343788676 scopus 로고    scopus 로고
    • note
    • Some of the lipase-containing gels with low loading are commercially available from Fluka (Switzerland).


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.