In vivo synthesis and purification of radioactive p- hydroxybenzylglucosinolate in Sinapis alba L.

Phytochemical Analysis

Volumn 11, Issue 3, 2000, Pages 174-178

  Chen, Sixue ^a   Halkier, Barbara Ann ^a  

^a UNIVERSITY OF COPENHAGEN   (Denmark)

Author keywords

Biosynthesis; Glucosinolates; In vivo radiolabelling; P Hydroxybenzylglucosinolate; Sinapis alba

Indexed keywords

AMINO ACIDS; BIOCHEMISTRY; ION EXCHANGE; PLANTS (BOTANY); PURIFICATION;

AMINO-ACIDS; BIOLOGICAL STUDIES; GLUCOSINOLATES; IN VIVO RADIOLABELING; IN-VIVO; NATURAL PLANTS; P-HYDROXYBENZYLGLUCOSINOLATE; PLANT PRODUCTS; RADIOLABELLING; SINAPIS ALBA;

BIOSYNTHESIS;

4 HYDROXYBENZYLGLUCOSINOLATE; CARBON 14; GLUCOSINOLATE; JASMONIC ACID; METHANOL; NATURAL PRODUCT; PLANT EXTRACT; TRITIUM; TYROSINE; UNCLASSIFIED DRUG;

ARTICLE; IN VIVO STUDY; ION EXCHANGE CHROMATOGRAPHY; ISOLATION PROCEDURE; ISOTOPE LABELING; NONHUMAN; PLANT LEAF; PURIFICATION; SYNTHESIS;

BRASSICALES; SINAPIS ALBA;

EID: 0034098169     PISSN: 09580344     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1099-1565(200005/06)11:3<174::AID-PCA513>3.0.CO;2-Q     Document Type: Article

References (13)

1. Bennett RN, Kiddle G, Hick AJ, Dawson GW, Wallsgrove RM. 1996. Distribution and activity of microsomal NADPH-dependent monooxygenases and amino acid decarboxylases in cruciferous and non-cruciferous plants, and their relationship to foliar glucosinolate content. Plant Cell Environ 19: 801-812.
2. 3H]-labelled glucosbrassicin, a potential radiotracer for metabolic studies of indole glucosinolates. J Lab Compd Radiopharm 33: 671-679.
3. Clossais-Besnard N, Larher F. 1991. Physiological role of glucosinolates in Brassica napus. Concentration and distribution pattern of glucosinolates among plant organs during a complete life cycle. J Sci Food Agric 56: 25-38.
4. Du L, Halkier BA. 1998. Biosynthesis of glucosinolates in the developing silique walls and seeds of Sinapis alba. Phytochemistry 48: 1145-1150.
5. Du L, Lykkesfeldt J, Olsen CE, Halkier BA. 1995. Involvement of cytochrome P450 in oxime production in glucosinolate biosynthesis as demonstrated by an in vitro microsomal enzyme system isolated from jasmonic acid-induced seedlings of Sinapis alba L. Proc Natl Acad Sci USA 92: 12505-12509.
6. Fieldsend J, Milford GF. 1994. Changes in glucosinolates during crop development in single-and double-low genotypes of winter oilseed rape (Brassica napus): I. Production and distribution in vegetative tissues and developing pods during development and potential role in recycling of sulphur within the crop. J Ann Appl Biol 124: 531-542.
7. Gijzen M, McGregor I, Séguin-Swartz G. 1989. Glucosinolate uptake by developing rapeseed embryos. Plant Physiol 89: 260-263.
8. Halkier BA. 1999. Glucosinolates. In Naturally Occurring Glycosides: Chemistry, Distribution and Biological Properties, Ikan R, (ed.). Wiley: Chichester; 193-223.
9. Porter AJR, Morton AM, Kiddle G, Doughty KJ, Wallsgrove RM. 1991. Variation in glucosinolate content of oilseed rape (Brassica napus L.) leaves. I. Effect of leaf age and position. Ann Appl Biol 118: 461-467.
10. Sørensen H. 1990. Glucosinolates: structure, properties and function. In Canola and Rapeseed. Production, Chemistry, Nutrition and Processing Technology. Shahidi F, (ed.). Van Nostrand Reinhold: New York; 149-172.
11. 14C]desulphophenylethylglucosinolate in Nasturtium officinale. Phytochemistry 44: 1251-1255.
12. Zhao F, Evans EJ, Bilsborrow PE, Syers JK. 1993. Influence of sulphur and nitrogen on seed yield and quality of low glucosinolate oilseed rape (Brassica napus L.) J Sci Food Agric 63: 29-37.
13. Zhao F, Evans EJ, Bilsborrow PE, Syers JK. 1994. Influence of nitrogen and sulphur on the glucosinolate profile of rapeseed (Brassica napus L.) J Sci Food Agric 64: 295-304.