Immunocytochemical localization and secretion process of the toxin CSTX-1 in the venom gland of the wandering spider Cupiennius salei (Araneae: Ctenidae)

Cell and Tissue Research

Volumn 299, Issue 3, 2000, Pages 417-426

  Malli, Heinz ^a   Kuhn Nentwig, Lucia ^a   Imboden, Hans ^a   Moon, Myung Jin ^b   Wyler, Toni ^a  

^a UNIVERSITY OF BERN   (Switzerland)

^b Dankook University   (South Korea)

Author keywords

Immunocytochemistry; Monoclonal antibody; Spider, Cupiennius salei (Araneae); Toxin; Venom gland

Indexed keywords

FLUORESCEIN; HORSERADISH PEROXIDASE; MONOCLONAL ANTIBODY; SPIDER VENOM;

ANIMAL TISSUE; ARTICLE; CELL SECRETION; CELLULAR DISTRIBUTION; FEMALE; IMMUNOCYTOCHEMISTRY; IMMUNOFLUORESCENCE; NONHUMAN; PRIORITY JOURNAL; SPIDER;

ADAPTATION, PHYSIOLOGICAL; ANIMALS; ANTIBODIES, MONOCLONAL; BLOTTING, WESTERN; CYTOPLASMIC GRANULES; ELECTRIC STIMULATION; EXOCRINE GLANDS; FLUORESCEIN-5-ISOTHIOCYANATE; FLUORESCENT DYES; IMMUNOHISTOCHEMISTRY; MICROSCOPY, ELECTRON; SPIDER VENOMS; SPIDERS;

ANIMALIA; ARANEAE; ARMORACIA RUSTICANA; CTENIDAE; CUPIENNIUS SALEI; INVERTEBRATA;

EID: 0034097505     PISSN: 0302766X     EISSN: None     Source Type: Journal    
DOI: 10.1007/s004410050040     Document Type: Article

References (27)

1. Bettini S, Maroli M (1978) Venoms of Therididae genus Latrodectus. In: Bettini S (ed) Handbook of experimental pharmacology, vol. 48. Springer, Berlin, Heidelberg, New York, pp 149-212
2. Cavalieri M, Corvaja N, Grasso A (1990) Immunocytological localization by monoclonal antibodies of α-latrotoxin in the venom gland of the spider Latrodectus tredecimguttatus. Toxicon 28:341-346
3. Coddington JA, Levi HW (1991) Systematics and evolution of spiders (Araneae). Annu Rev Ecol Syst 22:565-592
4. Foelix RF (1996) Biology of spiders, 2nd edn. Oxford University Press, Oxford
5. Foil LD, Coons LB, Norment BR (1979) Ultrastructure of the venom gland of the brown recluse spider, Loxosceles reclusa Gertsch and Mulaik (Araneae: Loxoscelidae). Int J Insect Morphol Embryol 8:325-334
6. Friedel T, Nentwig W (1989) Immobilizing and lethal effects of spider venoms on the cockroach and the common meal beetle. Toxicon 27:305-316
7. Imboden H, Felix D (1995) Immunocytochemistry in brain tissue. In: Phillips MI, Dwight E (eds) Methods in neurosciences. Academic, New York, pp 236-260
8. Järlfors U, Smith DS, Russell FE (1969) Nerve endings in the venom gland of the spider Latrodectus mactans. Toxicon 7:263-265
9. Järlfors U, Smith DS, Russell FE (1971) Innervation of the venom-secreting cells in the black widow spider (Latrodectus mactans). In: Vries A de, Kochva E (eds) Toxins of animal and plant origin. Gordon and Breach, New York, pp 159-179
10. Kovoor J, Zylberberg L (1971) Histologie et infrastructure de la glande chélicérienne de Pholcus phalangoides Fuessl., a divers stades du cycle sécrétoire. Arch Zool Exp Gen 112:117-134
11. Kuhn-Nentwig L, Malli H (1996) Purification and comparison of two toxic peptides (CSTX-1 and CSTX-2) in the venom of Cupiennius salei (Ctenidae). Rev Suisse Zool :371-375
12. Kuhn-Nentwig L, Schaller J, Nentwig W (1994) Purification of toxic peptides and the amino acid sequence of CSTX-1 from the multicomponent venom of Cupiennius salei (Araneaea: Ctenidae). Toxicon 32:287-302
13. Kuhn-Nentwig L, Bücheler A, Studer A, Nentwig W (1998) Taurine and histamine: low molecular compounds in prey hemolymph increase the killing power of spider venom. Naturwissenschaften 85:136-138
14. Larue DT, Winer JA (1996) Postembedding immunocytochemistry of large sections of brain tissue: an improved flat embedding technique. J Neurosci Methods 68:125-132
15. Malli H, Vapenik Z, Nentwig W (1993) Ontogenetic changes in the toxicity of the venom of the spider Cupiennius salei (Araneae: Araneidae). Zool Jb Physiol 97:113-122
16. Malli H, Imboden H, Kuhn-Nentwig L (1998) Quantifying the venom dose of the spider Cupiennius salei using monoclonal antibodies. Toxicon 36:1959-1969
17. Malli H, Kuhn-Nentwig L, Imboden H, Nentwig W (1999) Effects of size, motility and paralysation time of prey on the quantity of venom injected by the hunting spider Cupiennius salei. J Exp Biol 202:2083-2089
18. McLean I, Nakane PK (1974) Periodate-lysine-paraformaldehyde fixative a new fixative for immunoelectron microscopy. J Histochem Cytochem 22:1077-1083
19. Millot J (1931) Les glandes venimeuses des aranéides. Ann Sci Nat Zool 10:113-147
20. Moon MJ (1996) Fine structural analysis of the neuromuscular junction in the venomous organ of the spider, Agelena limbata (Araneae: Agelenidae). Korean J Zool 39:223-230
21. Ori M, Ikeda H (1998) Spider venoms and spider toxins. J Toxicol 17:405-426
22. Reynolds ES (1963) The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J Cell Biol 17:208
23. Russell FE, Järlfors U, Smith DS (1973) Preliminary report on the fine structure of the venom gland of the tarantula. Toxicon 11:439-440
24. Smith DS, Russell FE (1967) Structure of the venom gland of the black widow spider Latrodectus mactans. A preliminary light and electron microscopic study. In: Russell FE, Saunders PR (eds) Animal toxins. Pergamon, Oxford, pp 1-15
25. Spurr AR (1969) A low-viscosity epoxy resin embedding medium for electron microscopy. J Ultrastruct Res 26:21-43
26. Wilson MB, Nakane PK (1978) Recent developments in the periodate method of conjugating horseradish peroxidase (HRPO) to antibodies. In: Knapp W, Holubar K, Wick G (eds) Immunofluorescence and related staining techniques. Elsevier, Amsterdam, pp 215-224
27. Zierz R (1990) Fluorchromkoppelung monoklonaler Antikörper. In: Peters JH, Baumgarten H (eds) Monoklonale Antikörper. Springer, Berlin Heidelberg, New York, pp 303-305