Electrophysiological characterization of human keratinocytes using the patch-clamp technique

Experimental Dermatology

Volumn 9, Issue 3, 2000, Pages 219-223

  Wohlrab, David ^a   Wohlrab, J ^a   Markwardt, F ^a  

^a MARTIN LUTHER UNIVERSITY HALLE WITTENBERG   (Germany)

Author keywords

DIDS; Ionic channels; Keratinocytes

Indexed keywords

4,4' DIISOTHIOCYANATOSTILBENE 2,2' DISULFONIC ACID; ION CHANNEL; POTASSIUM;

ARTICLE; CELL DIFFERENTIATION; CELL MEMBRANE CONDUCTANCE; CELL MEMBRANE POTENTIAL; CELL PROLIFERATION; DRUG MECHANISM; HUMAN; HUMAN CELL; KERATINOCYTE; PATCH CLAMP; POTASSIUM CELL LEVEL;

CELL DIVISION; CELL LINE; CHLORIDES; ELECTROPHYSIOLOGY; HUMANS; ION CHANNELS; KERATINOCYTES; MEMBRANE POTENTIALS; PATCH-CLAMP TECHNIQUES; POTASSIUM;

EID: 0034093494     PISSN: 09066705     EISSN: None     Source Type: Journal    
DOI: 10.1034/j.1600-0625.2000.009003219.x     Document Type: Article

References (23)

1. Cooper K D, Hammerberg C, Baadsgaard O et al. IL-1 activity is reduced in psoriatic skin. Decreased IL-1 alpha and increased nonfunctional IL-1 beta. J Immunol 1990: 144: 4593-4603.
2. Grossman R M, Krueger J, Yourish D et al. Interleukin 6 is expressed in high levels in psoriatic skin and stimulates proliferation of cultured human keratinocytes. Proc Natl Acad Sci USA 1989: 86: 6367-6371.
3. Morhenn V B. Keratinocyte proliferation in wound healing and skin diseases. Immunol Today 1988: 9: 104-107.
4. Partridge M, Chantry D, Turner M, Feldmann M. Production of interleukin-1 and interleukin-6 by human keratinocytes and squamous cell carcinoma cell lines. J Invest Dermatol 1991: 96: 771-776.
5. Eichelberg D, Fuchs A. Calmodulin-antagonism inhibits human keratinocyte proliferation. Arch Dermatol Res 1998: 280: 323-324.
6. Hirokawa M, Hashimoto Y, Ohkuma N, Mizumoto T, Iizuka H. Reversible inhibition of keratinocyte thymidine incorporation by the calmodulin antagonist W-7. J Dermatol 1990: 17: 403-408.
7. Iizuka H, Hashimoto Y, Hirokawa M, Matsuo S, Mizumoto T, Ohkawara A. Pig-skin epidermal calmodulin: effects of antagonists of calmodulin on DNA synthesis of pig-skin epidermis. Arch Dermatol Res 1985: 278: 133-137.
8. Wollina U. Calmodulin in human skin-localization and interpretation. Arch Dermatol Res 1993: 285: 310.
9. Green H. Cyclic AMP in relation to proliferation of the epidermal cell: a new view. Cell 1978: 15: 801-811.
10. Kuroki T, Ito T, Hosomi J, Munakata K, Uchida T, Nagai Y. Cyclic AMP as a mitotic signal for epidermal keratinocytes, but not for dermal fibroblasts. Cell Struct Funct 1982: 7: 295-305.
11. Kane C J, Knapp A M, Mansbridge J N, Hanawalt P C. Transforming growth factor-beta 1 localization in normal and psoriatic epidermal keratinocytes in situ. J Cell Physiol 1990: 144: 144-150.
12. Sharpe R J, Arndt K A, Bauer S I, Maione T E. Cyclosporine inhibits basic fibroblast growth factor-driven proliferation of human endothelial cells and keratinocytes. Arch Dermatol 1989: 125: 1359-1362.
13. Amigorena S, Choquet D, Teillaud L J, Korn H, Fridman H W. Ion channels and B cell mitogenesis. Mol Immunol 1990: 27: 1259-1268.
14. + channels and mitogenesis. Prog Clin Biol Res 1990: 334: 2-21.
15. Dubois M J, Rouzaire-Dubois B. Role of potassium channels in mitogenesis. Prog Biophys Mol Biol 1993: 59: 1-21.
16. Nilius B, Droogmans G. A role for potassium channels in cell proliferation? News in Physiological Sciences 1994: 16: 1-12.
17. Wohlrab D. Der Einfluß elektrophysiologischer Membraneigenschaften auf die Proliferation humaner Keratinozyten. Tectum Verlag Marburg 1998, ISBN 3-8288-0400-4.
18. Boukamp B, Petrussevska R T, Breitkreutz D, Hornung J, Markham A, Fusenig N E. Normal keratinization in a spontaneously immortalized aneuploid human keratinocyte cell line. J Cell Biol 1988: 106: 761-771.
19. Mille B. Ionic channels of excitable membranes. Sunderland, MA, USA: 1992. Sinauer Associates, Inc.
20. Foà P P, Walsh M F. Ion channels and ion pumps. Berlin, Heidelberg, New York: Springer, 1996: 317-335.
21. Mauro M T, Pappone A P, Isseroff R R. Extracellular calcium affects the membrane currents of cultured human keratinocytes. J Cell Physiol 1990: 143: 13-20.
22. Mauro T M, Pappone P A, Isseroff R R. Membrane currents in cultured human keratinocytes. J Cell Biol 1988-107: 355a.
23. 2+-induced differentiation. J Invest Dermatol 1997: 108: 864-870.