Purification and characterization of isoamyl acetate-hydrolyzing esterase encoded by the IAH1 gene of Saccharomyces cerevisiae from a recombinant Escherichia coli

Applied Microbiology and Biotechnology

Volumn 53, Issue 5, 2000, Pages 596-600

  Fukuda, K ^a   Kiyokawa, Y ^a   Yanagiuchi, T ^a   Wakai, Y ^a   Kitamoto, K ^b   Inoue, Y ^c   Kimura, A ^c  

^a Kizakura Sake Brewing Co Ltd   (Japan)

^b UNIVERSITY OF TOKYO   (Japan)

^c KYOTO UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

4 CHLOROMERCURIBENZOIC ACID; ACETIC ACID ISOBUTYL ESTER; DYFLOS; ESTERASE; ISOPENTYL ACETATE; MERCURY; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ARTICLE; ENZYME PURIFICATION; ESCHERICHIA COLI; FOOD INDUSTRY; GENE EXPRESSION REGULATION; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; SACCHAROMYCES CEREVISIAE;

ESCHERICHIA COLI; NEGIBACTERIA; SACCHAROMYCES CEREVISIAE;

EID: 0034091480     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s002530051662     Document Type: Article

References (27)

1. Ashida S, Ichikawa E, Suginami K, Imayasu S (1987) Isolation and application of mutants producing sufficient isoamyl acetate, a sake flavor component. Agric Biol Chem 51: 2061-2065
2. Blow D (1990) More catalytic triad. Nature 343: 694-695
3. Brady L, Brzozowski AM, Derewenda ZS, Dodson E, Dodoson G, Tolley S, Turkenburg JP, Christiansen L, Huge-Jensen B, Norskov L, Thim L, Menge U (1990) A serine protease triad forms the catalytic center of triacylglycerol lipase. Nature 343: 767-770
4. Fukuda K, Kuwahata O, Kiyokawa Y, Yanagiuchi T, Wakai Y, Kitamoto K, Inoue Y, Kimura A (1996) Molecular cloning and nucleotide sequence of the isoamyl acetate-hydrolyzing esterase gene (EST2) from Saccharomyces cerevisiae. J Ferment Bioeng 82: 8-15
5. Fukuda K, Yamamoto N, Kiyokawa Y, Yanagiuchi T, Wakai Y, Kitamoto K, Inoue Y, Kimura A (1998a) Brewing properties of sake yeast whose EST2 gene encoding isoamyl acetate-hydrolyzing esterase was disrupted. J Ferment Bioeng 85: 101-106
6. Fukuda K, Yamamoto N, Kiyokawa Y, Yanagiuchi T, Wakai Y, Kitamoto K, Inoue Y, Kimura A (1998b) Balance of activities of alcohol acetyltransferase and esterase in Saccharomyces cerevisiae is important for production of isoamyl acetate. Appl Environ Microbiol 64: 4076-4078
7. Fujii T, Nagasawa N, Iwamatu A, Bogaki T, Tamai Y, Hamachi M (1994) Molecular cloning, sequence analysis and expression of the yeast alcohol acetyltransferase gene. Appl Environ Microbiol 60: 2786-2792
8. Hoshino T, Sasaki T, Watanabe Y, Nagasawa T, Yamane T (1992) Purification and some characteristics of extracellular lipase from Fusarium oxysporium f. sp. lini. Biosci Biotech Biochem 56: 660-664
9. Inoue Y, Trevanichi S, Fukuda K, Izawa S, Wakai Y, Kimura A (1997) Role of esterase and alcohol acetyltransferase on production of isoamyl acetate in Hansenula mrakii. J Agric Food Chem 45: 644-649
10. Kawaguchi Y, Honda H, Taniguchi-Morimura J, Iwasaki S (1989) The codon CUG is read as serine in an asporogenic yeast Candida cylindracea. Nature 341: 164-166
11. Kuriyama I, Ashida S, Saito Y, Hata Y, Sugiyama K, Imayasu S (1986) Ethyl caproate synthesis and hydrolysis activity of sake yeast. Hakkokogaku 64: 175-180
12. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685
13. Lowry OH, Rosebrough NJ, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193: 265-275
14. Malcorps P, Dufour JP (1992) Short-chain and medium chain aliphatic-ester synthesis in Saccharomyces cerevisiae. Eur J Biochem 210: 1015-1022
15. Malcorps P, Chevol JM, Jamil S, Dufour JP (1991) A new model for the regulation of ester synthesis by alcohol acetyltransferase in Saccharomyces cerevisiae during fermentation. J Am Soc Brew Chem 49: 47-53
16. Minetoki T, Bogaki T, Iwamatu A, Fujii T, Hamachi M (1993) The purification, properties and internal peptide sequences of alcohol acetyltransferase isolated from Saccharomyces cerevisiae Kyokai no. 7. Biosci Biotech Biochem 57: 2094-2098
17. Ohnishi K, Yoshida Y, Toita J, Sekiguchi J (1994) Purification and characterization of novel lipolytic enzyme from Aspergillus oryzae. J Ferment Bioeng 78: 413-419
18. Parkkinen E, Suomalainen H (1982) Esterase of baker's yeast. III. The ester/acid ratio in model solution. J Inst Brew 88: 34-38
19. Schermers FH, Duffus JH, Macleod M (1976) Studies on yeast esterase. J Inst Brew 82: 170-174
20. Schrag JD, Li Y, Wu S, Cygler M (1991) Ser-His-Glu triad forms the catalytic site of lipase from Geotrichum candidum. Nature 351: 761-764
21. Shimada Y, Koga C, Sugihara A, Nagao T, Takada N, Tsunasawa S, Tominaga S (1993) Purification and characterization of novel solvent-tolerant lipase from Fasarium heterosporium. J Ferment Bioeng 75: 349-352
22. Soumalainen H (1981) Yeast esterases and aroma esters in alcohol beverages. J Inst Brew 82: 296-300
23. Wakai Y, Yanagiuchi T, Kiyokawa Y (1990) Properties of an isoamyl acetate hydrolytic enzyme from sake yeast strain. Hakkokogaku 68: 101-105
24. Yanagiuchi T, Kiyokawa Y, Wakai Y (1989a) Isolation of sake-yeast strains accumulating large amounts of isoamyl acetate productivity. Hakkokogaku 67: 159-165
25. Yanagiuchi T, Kiyokawa Y, Wakai Y (1989b) Isoamyl acetate accumulation in sake mash and isoamyl acetate hydrolysis activity of sake-yeast strains. Hakkokogaku 67: 419-425
26. Yoshioka K, Hashimoto N (1981) Ester formation by alcohol acetyltransferase from brewer's yeast. Agric Biol Chem 45: 2183-2190
27. Yoshioka K, Hashimoto N (1983) Cellular fatty acid and ester formation by brewer's yeast. Agric Biol Chem 47: 2287-2297