Evidence that actin and myosin are involved in the poleward flux of tubulin in metaphase kinetochore microtubules of crane-fly spermatocytes

Journal of Cell Science

Volumn 113, Issue 4, 2000, Pages 597-609

  Silverman Gavrila, R V ^a   Forer, A ^a  

^a YORK UNIVERSITY   (Canada)

Author keywords

Acetylated tubulin; Actin; Actomyosin; Kinetochore microtubule; Metaphase; Spindle; Spindle matrix; Tubulin flux

Indexed keywords

ACTIN; CYTOCHALASIN D; DIACETYL OXIME; F ACTIN; JASPAMIDE; LATRUNCULIN B; MYOSIN; NOCODAZOLE; PHALLOIDIN; PROTEIN SUBUNIT; SWINHOLIDE A; TUBULIN;

ACETYLATION; ANIMAL CELL; ARTICLE; CENTROMERE; CONTROLLED STUDY; FLY; MALE; METAPHASE; MICROTUBULE; MITOSIS SPINDLE; NONHUMAN; PRIORITY JOURNAL; PROTEIN TRANSPORT; SPERMATOCYTE;

ANIMALIA; TIPULIDAE;

EID: 0034090944     PISSN: 00219533     EISSN: None     Source Type: Journal    
DOI: 10.1242/jcs.113.4.597     Document Type: Article

References (11)

1. Ayscough, K. (1998). Use of latrunculin A - an actin monomer binding drug. Meth. Enzymol. 298, 18-25.
2. Bednara, J., Willemse, M. T. M. and van Lammeren, A. A. M. (1990). Organization of the actin cytoskeleton during megasporogenesis in Gasteria verrucosa visualized with fluorescent-labelled phalloidin. Acta Bot. Neerl. 39, 43-48.
3. Bubb, M. R., Spector, I., Bershadsky, A. D. and Korn, E. D. (1995). Swinholide A is a microfilament disrupting marine toxin that stabilizes actin dimers and severs actin filaments. J. Biol. Chem. 270, 3463-3466.
4. Bubb, M. R. and Spector, I. (1998). Use of F-actin binding drugs, misakinolide A and swinholide A. Meth. Enzymol. 298, 26-32.
5. Bubb, M. R. (1994). Jasplakinolide, a cytotoxic natural product induces actin polymerization and competitively inhibits the binding of phalloidin to F-actin. J. Biol. Chem. 269, 14869-14871.
6. Canman, J. C. and Bement, W. M. (1997). Microtubules suppress actomyosin-based flow in Xenopus oocytes. J. Cell Sci. 110, 1907-1917.
7. Cleary, A. L. (1995). F-actin redistributions at the division site in living Tradescantia stomatal complexes as revealed by microinjection of rhodamine-phalloidin. Protoplasma 185, 152-165.
8. Cooper, J. (1987). Effects of cytochalasin and phalloidin on actin. J. Cell Biol. 105, 1473-1478.
9. Correas, I., Padilla, R. and Avila, J. (1990). The tubulin-binding sequence of brain microtubule-associated proteins, tau and MAP-2, is also involved in actin binding. Biochem. J. 269, 61-64.
10. Coue, M., Brenner, S., Spector, I. and Korn, E. (1987). Inhibition of actin polymerization by latrunculin A. FEBS Lett. 213, 316-318.
11. Cramer, L. P. and Mitchison, T. J. (1995). Myosin is involved in postmitotic cell spreading. J. Cell Biol. 131, 179-189.