Iron enhances hepatitis C virus replication in cultured human hepatocytes

Liver

Volumn 20, Issue 2, 2000, Pages 125-128

  Kakizaki, Satoru ^a   Takagi, Hitoshi ^a   Horiguchi, Norio ^a   Toyoda, Mitsuo ^a   Takayama, Hisashi ^a   Nagamine, Takeaki ^a   Mori, Masatomo ^a   Kato, Nobuyuki ^b  

^a GUNMA UNIVERSITY   (Japan)

^b NATIONAL CANCER CENTER RESEARCH INSTITUTE   (Japan)

Author keywords

Hepatitis C virus; Iron; PH5CH8

Indexed keywords

3 (4,5 DIMETHYL 2 THIAZOLYL) 2,5 DIPHENYLTETRAZOLIUM BROMIDE; IRON; VIRUS RNA;

ARTICLE; ASSAY; CELL VIABILITY; CONTROLLED STUDY; CYTOTOXICITY; HEPATITIS C; HEPATITIS C VIRUS; HUMAN; HUMAN CELL; IRON OVERLOAD; LIVER CELL CULTURE; NONHUMAN; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; VIRUS REPLICATION;

CELL LINE, TRANSFORMED; CELL SURVIVAL; COLORING AGENTS; DNA PRIMERS; HEPACIVIRUS; HUMANS; IRON; LIVER; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, VIRAL; TETRAZOLIUM SALTS; THIAZOLES; VIRUS REPLICATION;

EID: 0034087644     PISSN: 01069543     EISSN: None     Source Type: Journal    
DOI: 10.1034/j.1600-0676.2000.020002125.x     Document Type: Article

References (20)

1. HUDES B K, FABRY T L, KLION F M. Hepatic iron metabolism in chronic hepatitis C. Hepatology 1992; 16: 206A.
2. HAYASHI H, TAKIKAWA T, NISHIMURA N, YANO M, ISOMURA T, SAKAMOTO N. Improvement of serum aminotransferase levels after phlebotomy in patients with chronic active hepatitis C and excess hepatic iron. Am J Gastroenterol 1994; 89: 986-8.
3. BURT M J, COOKSLEY W G. The influence of iron on chronic hepatitis C. J Gastroenterol Hepatol 1998; 13: 330-3.
4. PIPERNO A, SAMPIETRO M, D'ALBA R, et al. Iron stores, response to α-interferon therapy, and effects of iron depletion in chronic hepatitis C. Liver 1996; 16: 248-54.
5. FONG T L, HAN S H, TSAI N C, et al. A pilot randomized, controlled trial of the effect of iron depletion on long-term response to α-interferon in patients with chronic hepatitis C. J Hepatol 1998; 28: 369-74.
6. KATO N, IKEDA M, MIZUTANI T, et al. Replication of hepatitis C virus in cultured non-neoplastic human hepatocytes. Jpn J Cancer Res 1996; 87: 787-92.
7. IKEDA M, SUGIYAMA K, MIZUTANI T, et al. Human hepatocyte clonal cell lines that support persistent replication of hepatitis C virus. Virus Res 1998; 56: 157-67.
8. NOGUCHI M, HIROHASHI S. Cell lines from non-neoplastic liver and hepatocellular carcinoma tissue from a single patient. In Vitro Cell Dev Biol 1996; 32: 135-7.
9. IKEDA M, KATO N, MIZUTANI T, SUGIYAMA K, TANAKA K, SHIMOTOHNO K. Analysis of the cell tropism of HCV by using in vitro HCV-infected human lymphocytes and hepatocytes. J Hepatol 1997; 27: 445-54.
10. MIZUTANI T, KATO N, IKEDA M, SUGIYAMA K, SHIMOTOHNO K. Long-term human T-cell culture system supporting hepatitis C virus replication. Biochem Biophys Res Commun 1996; 227: 822-6.
11. KATO N, HIJIKATA M, OOTSUYAMA M, et al. Molecular cloning of the human hepatitis C virus genome from Japanese patients with non-A, non-B hepatitis. Proc Natl Acad Sci USA 1990; 87: 9524-8.
12. MIZUTANI T, KATO N, SAITO S, IKEDA M, SUGIYAMA K, SHIMOTOHNO K. Characterization of hepatitis C virus replication in cloned cells obtained from a human T-cell leukemia virus type 1-infected cell line, MT-42. J Virol 1996; 70: 7219-23
13. MOSSMAN T. Rapid calorimetric assay for cell growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods 1983; 65: 55-63.
14. IKEDA M, SUGIYAMA TANAKA T, TANAKA K, et al. Lactoferrin markedly inhibits hepatitis C virus infection in cultured human hepatocytes. Biochem Biophys Res Commun 1998; 245: 549-53.
15. BONKOVSKY H L. Therapy of hepatitis C: other options. Hepatology 1997; 26: 143S-51.
16. KOUTSILIERI E, GOTZ ME, SOPPER S, et al. Regulation of glutathione and cell toxicity following exposure to neurotropic substances and human immunodeficiency virus-1 in vitro. J Neurovirol 1997; 3: 342-9.
17. FUCHS O. Effects of intracellular chelatable iron and oxidative stress on transcription of classical cellular glutathione peroxidase gene in murine erythroleukemia cells. Neoplasma 1997; 44: 184-91.
18. DAI Y, GOLD B, VISHWANATHA J K, RHODE S L. Mimosine inhibits viral DNA synthesis through ribonucleotide reductase. Virology 1994; 205: 210-6.
19. CINATL J JR, HOFFMANN F, CINATL J, et al. In vitro inhibition of human cytomegalovirus replication by calcium trinatrium diethylenetriaminepentaacetic acid. Antiviral Res 1996; 31: 23-4.
20. SCHTROV V A, BOELAERT J R, CHOUAIB S, DROGE W, LEHMANN V. Iron chelation decreases human immunodeficiency virus-1 Tat potentiated tumor necrosis factor-induced NF-kappa B activation in Jurkat cells. Eur Cytokine Netw 1997; 8: 37-43.