Chlorzoxazone: A probe drug whose metabolism can be used to monitor toluene exposure in rats

Archives of Toxicology

Volumn 74, Issue 3, 2000, Pages 139-144

  Mizuno, Dai ^a   Tanaka, Einosuke ^a   Tanno, Kozo ^a   Misawa, Shogo ^a  

^a UNIVERSITY OF TSUKUBA   (Japan)

Author keywords

Chlorzoxazone; CYP2E1; Cytochrome P450; Hepatic enzyme activity; Induction; Rat; Toluene

Indexed keywords

CHLORZOXAZONE; CYTOCHROME P450 INHIBITOR; DIETHYLDITHIOCARBAMIC ACID; DIMETHYLNITROSAMINE DEMETHYLASE; DRUG METABOLITE; OLIVE OIL; TOLUENE; UNSPECIFIC MONOOXYGENASE;

ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; BLOOD SAMPLING; CONTROLLED STUDY; DRUG CLEARANCE; DRUG ELIMINATION; DRUG EXPOSURE; DRUG HALF LIFE; DRUG HYDROXYLATION; DRUG METABOLISM; ENZYME ACTIVITY; MALE; MICROSOME; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; RAT;

EID: 0034085366     PISSN: 03405761     EISSN: None     Source Type: Journal    
DOI: 10.1007/s002040050666     Document Type: Article

References (35)

1. Bachmann K, Sarver JG (1996) Chlorzoxazone as a single sample probe of hepatic CYP2E1 activity in humans. Pharmacology 52: 169-177
2. Baelum J (1991) Human solvent exposure. Factors influencing the pharmacokinetics and acute toxicity. Pharmacol Toxicol 68 Suppl 1: 1-36
3. Dilger K, Metzler J, Bode JC, Klotz U (1997) CYP2E1 activity in patients with alcoholic liver disease. J Hepatol 27: 1009-1014
4. Eliasson E, Mkrtchian S, Ingelman-Sundberg M (1992) Hormone-and substrate-regulated intracellular degradation of cytochrome P450 (2E1) involving MgATP-activated rapid proteolysis in the endoplasmic reticulum membranes. J Biol Chem 267: 15765-15769
5. Girre C, Lucas D, Hispard E, Menez C, Dally S, Menez JF (1994) Assessment of cytochrome P4502E1 induction in alcoholic patients by chlorzoxazone pharmacokinetics. Biochem Pharmacol 47: 1503-1508
6. Imai Y, Ito A, Sato R (1966) Evidence for biochemically different types of vesicles in the hepatic microsomal fraction. J Biochem 60: 417-428
7. Kim RB, O'Shea D, Wilkinson GR (1995) Interindividual variability of chlorzoxazone 6-hydroxylation in men and women and its relationship to CYP2E1 genetic polymorphisms. Clin Pharmacol Ther 57: 645-655
8. Kim H, Wang RS, Elovaara E, Raunio H, Pelkonen O, Aoyama T, Vainio H, Nakajima T (1997) Cytochrome P450 isozymes responsible for the metabolism of toluene and styrene in human liver microsomes. Xenobiotica 27: 657-665
9. Koop DR (1992) Oxidative and reductive metabolism by cytochrome P450 2E1. FASEB J 6: 724-7310
10. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685
11. Lowry OH, Roseberough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193: 265-275
12. Lucas D, Farez C, Bardou LG, Vaisse J, Attali JR, Valensi P (1998) Cytochrome P450 2E1 activity in diabetic and obese patients as assessed by chlorzoxazone hydroxylation. Fundam Clin Pharmacol 12: 553-558
13. Mishin VM, Rosman AS, Basu P, Kessova I, Oneta CM, Lieber CS (1998) Chlorzoxazone pharmacokinetics as a marker of hepatic cytochrome P4502E1 in humans. Am J Gastroenterol 93: 2154-2161
14. Nakajima T, Wang RS (1994) Induction of cytochrome P450 by toluene. Int J Biochem 26: 1333-1340
15. Nakajima T, Wang RS, Elovaara E, Park SS, Gelboin HV, Hietanen E, Vainio H (1991) Monoclonal antibody-directed characterization of cytochrome P450 isozymes responsible for toluene metabolism in rat liver. Biochem Pharmacol 41: 395-404
16. Nakajima T, Wang RS, Elovaara E, Gonzalez FJ, Gelboin HV, Raunio H, Pelkonen O, Vainio H, Aoyama T (1997) Toluene metabolism by cDNA-expressed human hepatic cytochrome P450. Biochem Pharmacol 53: 271-277
17. National Police Agency (1998) White paper on police (Annual case reports of drug and toxic poisoning in Japan). pp 110-115 (in Japanese)
18. Newton DJ, Wang RW, Lu AYH (1995) Cytochrome P450 inhibitors evaluation of specificities in the in vitro metabolism of therapeutics agents by human liver microsomes. Drug Metab Dispos 23: 154-158
19. Omura T, Sato R (1964) The carbon monoxide-binding pigment of liver microsomes: solubilization, purification, and properties. J Biol Chem 239: 2379-2385
20. O'Brian ET, Yeoman WB, Hobby JAE (1971) Hepatorenal damage from toluene in a "glue sniffer". Br Med J 2: 29-30
21. Pelkonen O, Maenpaa J, Taavitsainen P, Rautio A, Raunio H (1998) Inhibition and induction of human cytochrome P450 (CYP) enzymes. Xenobiotica 28: 1203-1253
22. Peter R, Bocker R, Beaune PH, Iwasaki M, Guengerich FP, Yang CS (1990) Hydroxylation of chlorzoxazone as a specific probe for human liver cytochrome P-450IIE1. Chem Res Toxicol 3: 566-573
23. Pyykkö K (1983a) Age-and sex-related differences in rat liver microsomal enzymes and their inducibility by toluene. Acta Pharmacol Toxicol 53: 401-409
24. Pyykkö K (1983b) Time course of effects of toluene on microsomal enzymes in rat liver, kidney and lung during and after inhalation exposure. Chem-Biol Interact 44: 299-310
25. Raucy JL (1995) Risk assessment: toxicity from chemical exposure resulting from enhanced expression of CYP2E1. Toxicology 105: 217-224
26. Raucy JL, Schultz ED, Wester MR, Arora S, Johnston DE, Omdahl JL, Carpenter SP (1997) Human lymphocyte cytochrome P450 2E1, a putative marker for alcohol-mediated changes in hepatic chlorzoxazone activity. Drug Metab Dispos 25: 1429-1435
27. Rea TM, Nash JF, Zabik JE, Born GS, Kessler WV (1984) Effects of toluene inhalation on brain biogenic amines in the rat. Toxicology 31: 143-150
28. Rees DC, Knisely JS, Jordan S, Balster R (1987) Discriminative stimulus properties of toluene in the mouse. Toxicol Appl Pharmacol 88: 97-104
29. Shimada T, Mimura M, Inoue K, Nakamura S, Oda H, Ohmori S, Yamazaki H (1997) Cytochrome P450-dependent drug oxidation activities in liver microsomes of various animal species including rats, guinea pigs, dogs, monkeys, and humans. Arch Toxicol 71: 401-408
30. Song BJ (1996) Ethanol-inducible cytochrome P450 (CYP2E1): biochemistry, molecular biology and clinical relevance: 1996 update. Alcohol Clin Exp Res 20 (Suppl 8): 138A-146A
31. Towbin H, Staehelin T, Gordon J (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA 76: 4350-4354
32. Vesell ES, Seaton TD, A-Rahim YI (1995) Studies on interindividual variations of CYP2E1 using chlorzoxazone as an in vivo probe. Pharmacogenetics 5: 53-57
33. Wang RS, Nakajima T (1991) Kinetics studies on toluene metabolism in ethanol- and phenobarbital-induced rat liver microsomes in vitro. Arch Toxicol 65: 39-44
34. Wang RS, Nakajima T, Park SS, Gelboin HV, Murayama N (1993) Monoclonal antibody-directed assessment of toluene induction of rat hepatic cytochrome P450 isozymes. Biochem Pharmacol 46: 413-419
35. Wang RS, Nakajima T, Tsuruta H, Honma T (1996) Effect of exposure to four organic solvents on hepatic cytochrome P450 isozymes in rat. Chem Biol Interact 99: 239-252