Stability of esterases and total proteins during seed storage of perennial ryegrass (Lolium perenne L.) and their use for cultivar discrimination

Euphytica

Volumn 111, Issue 3, 2000, Pages 161-168

  Wouters, T C A E ^a   Booy, G ^a  

^a WAGENINGEN UNIVERSITY   (Netherlands)

Author keywords

Cultivar identification; Esterases; Isoelectric focusing (IEF); Lolium perenne L.; Seed storage; Total proteins

Indexed keywords

CHROMOSOME BANDING; CULTIVAR IDENTIFICATION; CULTIVAR; ENZYME ANALYSIS; ENZYME POLYMORPHISM; ENZYME STABILITY; ESTERASE; GENETIC CORRELATION; GENETIC MARKER; GENETIC SELECTION; ISOELECTRIC FOCUSING; PLOIDY; QUANTITATIVE ANALYSIS;

LOLIUM PERENNE;

EID: 0034079621     PISSN: 00142336     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1003810030414     Document Type: Article

References (13)

1. Booy, G., T.C.A.E. Wouters & Y. Kleyn-Noordijk, 1998. Identification of lily cultivars using isoelectric focusing of proteins from bulb scales or tissue culture bulblets. Plant Breeding 117: 57-62.
2. Cooke, R.J., 1992. Proposed ISTA standard reference method for the identification of varieties of peas and ryegrass by SDS-PAGE. In: R.J. Cooke (Ed), Handbook of variety testing. Electrophoresis testing. The International Seed Testing Association (ISTA), Cambridge. 2-7-2-10.
3. De Prins, H.C. & L. Van de Weghe, 1983. Ryegrass cultivar identification using electrofocusing on polyacrylamide gels. Seed Science and Technology 11: 257-266.
4. Ferguson, J.M. & D.F. Grabe, 1986. Identification of cultivars of perennial ryegrass by SDS-PAGE of seed proteins. Crop Science 26: 170-176.
5. Freeman, G.W. & F.A. Yoder Jr., 1991. Examination of Kentucky bluegrass blends using esterase isoenzyme elctrophoresis. Seed Science and Technology 19: 553-560.
6. Gardiner, S.E. & M.B. Forde, 1987. SDS polyacrylamide gel electrophoresis of grass seed proteins: a method for cultivar identification of pasture grasses. Seed Science and Technology 15: 663-674.
7. Gardiner, S.E., M.B. Forde & C.R. Slack, 1986. Grass cultivar identification by sodium dodecylsulphate polyacrylamide gel electrophoresis. New Zealand Journal of Agricultural Research 29: 193-206.
8. Möller, M. & W. Spoor, 1993. Discrimination and identification of Lolium species and cultivars by rapid SDS-PAG electrophoresis of seed storage proteins. Seed Science and Technology 21: 213-223.
9. Murphy, P.M., N. Culleton & T. Flaherty, 1990. Identification of grass seed cultivars by SDS polyacrylamide gel electrophoresis. Irish Journal of Agricultural Research 29: 117-127.
10. Nakamura, S., 1979. Separation of ryegrass species using electrophoretic patterns of seed proteins and enzymes. Seed Science and Technology 7: 161-168.
11. Rong-Huay, J., C. Yuan-Di, S. Hsien-Yi & S. Jong-Ching, 1984. Oven drying method for polyacrylamide gel slab packed in cellophane sandwich. Analytical Biochemstry 141: 348-350.
12. Spoor, W & J.M. Hay, 1979. Identification of Poa pratensis lines using electrophoresis of seed extracts. Seed Science and Technology 7: 467-474.
13. Van Dreven, F., G. Esselink & A. Houwing, 1990. Esterase isoenzyme differences between cultivars of Kentucky bluegrass (Poa pratensis L.) from seed extracts using isoelectric focusing. Plant Varieties and Seeds 3: 89-97.