Discrimination between complete and incomplete chromosome exchanges in X-irradiated human lymphocytes using FISH with pan-centromeric and chromosome specific DNA probes in combination with telomeric PNA probe

International Journal of Radiation Biology

Volumn 76, Issue 6, 2000, Pages 807-813

  Fomina, J ^a   Darroudi, F ^b   Boei, J J W A ^a   Natarajan, A T ^b  

^a LEIDEN UNIVERSITY MEDICAL CENTER   (Netherlands)

^b JA Cohen Institute Interuniversity Research Institute for Radiopathology and Radiation Protection   (Netherlands)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; CENTROMERE; CHROMOSOME; DNA PROBE; FLUORESCENCE IN SITU HYBRIDIZATION; HUMAN; HUMAN CELL; LYMPHOCYTE; MOLECULAR PROBE; PRIORITY JOURNAL; TELOMERE; X IRRADIATION;

EID: 0034078938     PISSN: 09553002     EISSN: None     Source Type: Journal    
DOI: 10.1080/09553000050028968     Document Type: Article

References (19)

1. BOEI, J. J. W. A., VERMEULEN, S. and NATARAJAN, A. T., 1998a, Dose-response curves for X-ray induced interchanges and interarm intrachanges in human lymphocytes using arm-specific probes for chromosome 1. Mutation Research, 404, 45-53.
2. BOEI, J. J. W. A., VERMEULEN, S., FOMINA, J. and NATARAJAN, A. T., 1998b, Detection of incomplete exchanges and interstitial fragments in X-irradiated human lymphocytes using a telomeric PNA probe. International Journal of Radiation Biology, 73, 599-603.
3. CORNFORTH, M. N., MEYNE, J., LITTELFIELD, L. G., BAILEY, S. M. and MOYZIS, R. K., 1989, Telomere staining of human chromosome and mechanism of radiation-induced dicentric formation. Radiation Research, 120, 205-212.
4. DURANTE, M., GEORGE, K., WU, H. and YANG, T. C., 1996, Rejoining and misrejoining of radiation-induced chromatin breaks. I. Experiments with human lymphocytes. Radiation Research, 145, 274-280.
5. FINNON, P., LLOYD, D. C. and EDWARDS, A. A., 1995, Fluorescence in situ hybridization detection of chromosome aberrations in human lymphocytes: applicability to biological dosimetry. International Journal of Radiation Biology, 68, 429-435.
6. JOHNSON, K. L., TUCKER, J. D. and NATH, J., 1998, Frequency, distribution and clonality of chromosome damage in human lymphocytes by multi-color FISH. Mutagenesis, 13, 217-227.
7. KNEHR, S., ZITZELSBERGER, H., BRASELMANN, H., NAHRSTEDT, U. and BAUCHINGER, M., 1996, Chromosome analysis by fluorescence in situ hybridization: further indications for a non-DNA-proportional involvement of single chromosomes in radiation-induced structural aberrations. International Journal of Radiation Biology, 70, 385-392.
8. KODAMA, Y., NAKANO, M., OHTAKI, K., DELONGCHAMP, R., AWA, A. A. and NAKAMURA, N., 1997, Estimation of minimal size of translocated chromosome segments detectable by fluorescence in situ hybridization. International Journal of Radiation Biology, 71, 35-39.
9. LANSDORP, P. M., VERWOERD, N. P., VAN DE RIJKE, F. M., DRAGOWSKA, V., LITTLE, M. T., DIRKS, R. W., RAAP, A. K. and TANKE, H. J., 1996, Heterogenity in telomere length of human chromosomes. Human Molecular Genetics, 5, 685-691.
10. NATARAJAN, A. T., VYAS, R. C., DARROUDI, F. and VERMEULEN, S., 1992, Frequencies of X-ray-induced chromosome translocations in human peripheral lymphocytes as detected by in situ hybridization using chromosome-specific DNA libraries. International Journal of Radiation Biology, 61, 199-203.
11. PERRY, P. and WOLFF, S., 1974, New Giemsa method for differential staining of sister chromatids. Nature, 251, 156-158.
12. SAVAGE, J. R. K. and SIMPSON, P. J., 1994a, On the scoring of FISH-"painted" chromosome exchange aberrations. Mutation Research, 307, 345-353.
13. SAVAGE, J. R. K. and SIMPSON, P. J., 1994b, FISH "painting" patterns resulting from complex exchanges. Mutation Research, 312, 51-60.
14. SIMPSON, P. J. and SAVAGE, J. R. K., 1995, Estimating the true frequency of X-ray-induced complex chromosome exchanges using fluorescence in situ hybridization. International Journal of Radiation Biology, 67, 37-45.
15. SIMPSON, P. J. and SAVAGE, J. R. K., 1996, Dose-response curves for simple and complex chromosome aberrations induced by X-rays and detected using fluorescence in situ hybridization. International Journal of Radiation Biology, 69, 429-436.
16. STEPHAN, G. and PRESSL, S., 1997, Chromosome aberrations in human lymphocytes analyzed by fluorescence in situ hybridization after in vitro irradiation, and in radiation workers, 11 years after an accidental radiation exposure. International Journal of Radiation Biology, 71, 293-299.
17. TUCKER, J. D. and SENFT, J. R., 1994, Analysis of naturally occurring and radiation-induced breakpoint locations in human chromosomes 1, 2 and 4. Radialion Research, 140, 31-36.
18. TUCKER, J. D., MORGAN, W. F., AWA, A. A., BAUCHINGER, M., BLAKEY, D., CORNFORTH, M. N., LITTLEFIELD, L. G., NATARAJAN, A. T. and SHASSERRE, C., 1995, A proposed system for scoring structural aberrations detected by chromosome painting. Cytogenetics and Cell Genetics, 68, 211-221.
19. WU, H., GEORGE, K. and YANG, T. C., 1998, Estimate of true incomplete exchanges using fluorescence in situ hybridization with telomere probes. International Journal of Radiation Biology, 73, 521-527.