Comparative analysis of metabolism of medium- and plasma perfused primary pig hepatocytes cultured around a 3-D membrane network

International Journal of Artificial Organs

Volumn 23, Issue 2, 2000, Pages 104-110

  Unger, Juliane K ^a   Catapano, G ^d   Horn, N A ^a   Schroers, A ^c   Gerlach, J C ^b   Rossaint, R ^a  

^a RWTH AACHEN UNIVERSITY   (Germany)

^b HUMBOLDT UNIVERSITY   (Germany)

^c UNIVERSITY OF STRATHCLYDE   (United Kingdom)

^d NONE

Author keywords

Bioartificial liver; Culture medium; Metabolism; Porcine hepatocytes; Xenogenic plasma

Indexed keywords

ARTIFICIAL ORGANS; BIOCONVERSION; BIOREACTORS; NUTRITION; PHYSIOLOGY;

'CURRENT; BIOARTIFICIAL LIVER; CLINICAL APPLICATION; COMPARATIVE ANALYZES; CULTURE MEDIUM; HEPATOCYTES; MEDIA COMPOSITION; PORCINE HEPATOCYTE; SUPPORT SYSTEMS; XENOGENIC PLASMA;

METABOLISM;

CELL ENZYME;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; ARTIFICIAL LIVER; BIOREACTOR; BIOTRANSFORMATION; CELL FUNCTION; CELL METABOLISM; CELL VIABILITY; CLINICAL TRIAL; CULTURE MEDIUM; ENZYME RELEASE; IN VITRO STUDY; LIVER CELL CULTURE; LIVER CELL MEMBRANE; NONHUMAN; NUTRITION; PLASMA; SWINE; TISSUE PERFUSION;

EID: 0034078808     PISSN: 03913988     EISSN: None     Source Type: Journal    
DOI: 10.1177/039139880002300207     Document Type: Article

References (18)

1. Riordan SM, Skouteris GG, Williams R. Metabolic activity and clinical efficacy of animal and human hepatocytes in bioartificial support systems of acute liver failure. Int J Artif Organs 1998; 21: 312-8.
2. Te Velde AA, Ladiges NCJJ, Flendrig LM, Chamuleau RAFM. Functional acticity of isolated pig hepatocytes attached to different extracellular matrix substrates. Implication for an application of pig hepatocytes in a bioartificial liver. J Hepatology 1995; 23: 184-92.
3. Gerlach JC, Schnoy N, Encke J, Smith MD, Müller C, Neuhaus P. Improved hepatocyte in vitro maintenance in a culture model with woven multicompartment capillary systems: electron microscopy studies. Hepatology 1995; 22: 546-52.
4. Morsiani E, Pazzi P, Moscioni AD, Rozga J, Azzena G, Demetriou AA. In vitro morphological and functional characterization of isolated porcine hepatocytes for extracorporeal liver support: Bile acid uptake and conjugation. J Surg Res 1998; 79: 54-60.
5. Sielaff TD, Hu MY, Rao S, et al. A technique for porcine hepatocyte harvest and description of differentiated metabolic functions in static culture. Transplantation 1995; 59: 1459-63.
6. Gerlach JC, Encke J, Hole O, Müller C, Ryan CJ, Neuhaus P. Bioreactor for a larger scale hepatocyte in vitro perfusion. Transplantation 1994; 58: 984-8.
7. Gerlach JC. Development of a hybrid liver support system: a review. Int J Artif Organs 1996; 19: 645-54
8. Gerlach J, Klöppel K, Schön MR, et al. Comparison of pig hepatocyte isolation using intraoperative perfusion without ischemia and isolation of cells from abattoir organs after warm ischemia. Artif Organs 1993; 17: 950-3.
9. Gerlach J, Brombacher J, Smith M, Neuhaus P. High yield hepatocyte isolation from pig livers for investigation of hybrid liver support systems: Influence of collagenase concentration and body weight. J Surg Res 1996; 62: 85-89.
10. Beinert WD, Meisner A, Fuchs M, Riedel E, Lüpke M, Brückner H. OPA or FMOC? - Amino acid analysis with the robot autosampler. GIT Fachz Lab 1992; 10: 1018.
11. Fuchs M, Gerlach J, Encke J, et al. Amino acid metabolism by hepatocytes in a hybrid liver support bioreactor. Int J Artif Organs 1994; 17: 663-9.
12. Baquerizo A, Mhoyan A, Kearns-Jonker M, et al. Characterization of human xenoreactive antibodies in liver failure patients exposed to pig hepatocytes after bioartificial liver treatment: an ex vivo model of pig to human transplantation. Transplant Proc 1997; 29: 964-5.
13. Cunningham JM. Hodgson HJF. Microcarrier culture of hepatocytes in whole plasma for use in liver support bioreactors. Int J Artif Organs 1992; 15: 162-7.
14. De Rudder D, Remon JP, Neyt EN. The sorption of nitroglycerin by infusion sets. J Pharm Pharmacol 1987; 39: 556-8.
15. Cawello W, Bonn R. The effect on biologic availability of the choice of infusion material in nitroglycerin therapy. Drug Res 1983; 33: 595-7.
16. Wu FJ, Peshwa MV, Cerra FB, Hu W-S. Entrapment of heaptocyte spheroids in a hollow fiber bioreactor as a potential bioartificial liver. Tissue Engineering 1995; 1: 29-40.
17. Flendrig LM, Maas MAW, Daalhuisen J, et al. Does the extend of the culture time of primary hepatocytes in a bioreactor affect the treatment efficacy of bioartificial liver? Int J Artif Organs 1998; 21: 542-7.
18. Flendrig LM, Sommeijer D, Ladiges NCJJ et al. Commercially available media for flushing extracorporeal bioartificial liver systems prior to connection to the patient's circulation: an in vitro comparative study in two and three dimensional porcine hepatocyte cultures. Int J Artif Organs 1998; 21: 467-72.